摘要
为了探索小麦-黑麦双二倍体形成过程中微卫星序列的变异情况,为异源多倍体进化及小麦育种研究提供理论参考,以来源于3个组合的小麦-黑麦杂交后代植株及亲本小麦、黑麦为材料,利用已有的小麦和黑麦微卫星标记调查小麦-黑麦双二倍体形成过程中微卫星序列的变化。结果表明,所用的126对小麦SSR引物中,除22对没有扩增出产物外,其余104对引物从每个组合的后代植株中扩增的带型与从其亲本小麦中扩增的带型完全相同。所用的27对黑麦SSR引物中,10对引物从每个组合的后代植株中扩增的带型与其从亲本黑麦中扩增的带型完全相同,10对引物在亲本黑麦、F1植株及3个双二倍体植株之间表现出了多态性,7对引物从亲本黑麦中扩增的条带在所有后代中缺失。说明小麦-黑麦异源多倍体化过程中小麦微卫星序列是相对稳定的,而黑麦微卫星序列比小麦微卫星序列更易受到异源多倍体化的影响。微卫星序列变化是伴随多倍体体化而快速发生的。
To investigate the microsatellite variation during wheat-rye allopolyploidization and to
provide references for studies on the evolution of allopolyploids and wheat breeding, 9 F1 hybrids and 9 derived amphiploids from three different cross combinations between wheat and rye were analyzed and compared with their direct parental plants using SSR markers. Of 126 wheat SSR markers, 104 markers amplified the same band pattern among F1 hybrids, amphiploids and wheat parents, however, the other 22 markers did not amplified products. Of 27 rye SSR markers, 10 markers amplified the same band pattern among F1hybrids, amphiploids and rye parents, 10 markers displayed polymorphism among F1 hybrids, amphiploids and rye parents, and 7 markers amplified products only from rye parents. These results indicate that microsatellite of rye is more conservative than those of wheat during allopolyploidization. Allopolyploidization in wheat-rye can be accompanied by rapid variation of microsatellite.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2008年第2期197-201,共5页
Journal of Triticeae Crops
基金
国家自然科学基金项目(30671136)
四川农业大学青年基金项目(00131300)
