摘要
目的比较两种不同种属的动物及不同免疫原诱发的实验性变态反应性脑脊髓炎（EAE）模型的各自特点及其适用的优缺点。方法应用兔脊髓匀浆（R-SCH）、SD大鼠脊髓匀浆（M-SCH）、异种牛大脑白质匀浆（C-SCH）为免疫原，分别免疫新西兰兔和SD大鼠制备EAE动物模型。观察比较模型的发病过程、临床评分、病理变化和病理组织学评分。结果新西兰兔EAE模型呈单相发病过程，发病时间长，发病率较低，但其病灶大且集中，MRI更易观察和定位。SD大鼠EAE模型则呈典型的双相病程，发病时间短，发病率高，复发率高，其发病更像人类多发性硬化的发病过程。同时发现同种免疫原诱发的EAE模型其发病过程更稳定，发病率也较异种免疫原高．其导致的模型病理变化也更明显，尤其是脱髓鞘更明显。结论不同的动物物种和不同的免疫原制备的EAE模型具有各自适用的优缺点和特点，可根据研究目的的不同选取所需的模型。
Objective To compare the characteristics and advantages and disadvantages of 2 different animal models （SD rats and New Zealand rabbits） of experimental allergic encephalomyelitis （EAE） induced with different antigens. Methods SD rats were immunized with homogeneous spinal cord homogenate （M-SCH） and cow spinal cord homogenate （C-SCH）, and New Zealand rabbits were immunized with homogeneous spinal cord homogenate （R-SCH） and C-SCH. The clinical course, the severity and histopathology and its scores were observed and compared between the 2 animal models. Results EAE induced in rabbits manifested an acute monophase course, long onset time, low incidence rate, big and collective pathological focus that help MRI to observe. Whereas, EAE induced in SD rats showed a remitting-relapsing biphase course, short onset time, high incidence rate and recurrence rate, which were more similar with multiple sclerosis （MS） in human beings. We also observed that the course was more steady in EAE induced with homogeneous immunogeus in different animals and that the changes were more salient in EAE course, especially in demyelination. Conclusion EAE models induced with different immunogens and in different animals have different characteristics, and therefore can be selectively used in various studies.
出处
《中华神经医学杂志》
CAS
CSCD
2007年第3期286-289,296,共5页
Chinese Journal of Neuromedicine
基金
广州市科技计划项目（2006J1-C0231）
番禺区科技计划项目（2006-Z-44-1）
广东省科技计划项目（2006B36004006）
关键词
实验性变态反应性脑脊髓炎
同种脊髓匀浆
动物模型
Experimental allergic encephalomyelitis
Homogeneous spinal cord homogenate
Animal models
