摘要
目的通过测定神经生长因子受体trkA在实验性变态反应性脑脊髓炎（EAE）模型中的含量变化以及探讨PG490在其中的作用和作用途径。方法48只新西兰兔在已建立EAE模型的基础上按随机数字表法分为3组，即模型组、PG490干预组1（首次发病期给予干预）及PG490干预组2（复发期给予干预）。采用ELISA法检测各组动物脑组织中trkA蛋白含量变化，并观察和比较各组临床学评分变化。结果EAE模型发病是呈缓解复发的过程，干预组其平均临床评分均较模型组明显降低，差异有统计学意义（P〈0．05）。PG490干预组1trkA蛋白含量与模型组相比较差异无统计学意义（P〉0．05）；PG490干预组2trkA蛋白含量与模型组相比较差异有统计学意义（P〈0．05）。结论PG490能够通过调节神经生长因子受体trkA含量减轻EAE模型的临床症状。
Objective To determine the changes of NGF receptor trkA content and investigate the role and the pathway of PG490 on trkA expression in experimental autoimmune encephalomyelitis （EAE） models. Methods Fourty-eight rabbit models were induced and randomized into model group, PG490 intervention group 1 （giving PG490 at the onset） and PG490 intervention group 2 （giving PG490 at the recurrence）. The clinical scores and pathological changes were observed and the expression changes of trkA in the brain tissue were tested by ELISA methods before and after the treatment with PG490. Results The recurrence in the model group was an extremely slow process. The mean clinical scores in the PG490 intervention groups were significantly lower as compared with those in the model group （P〈 0.05）. No obvious differences of the trkA content were found between the model group and the PG490 intervention group 1 （P〉0.05）; however, significant differences of the trkA content were noted between the model group and the PG490 intervention group 2 （P〈0.05）. Conclusion PG490 can improve the clinical symptoms in EAE models by adjusting the trkA content.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2010年第3期250-252,257,共4页
Chinese Journal of Neuromedicine
基金
基金项目：广东省中医药局科技项目（2007144）：广东省自然科学基金（8151018201000036）
广东省科技计划基金（20098030801363）
