Increasing stress from global warming, sea level rise, acidification, sedimentation, pollution, and unsustainable practices have degraded the most critical coastal ecosystems including coral reefs, oyster reefs, and s...Increasing stress from global warming, sea level rise, acidification, sedimentation, pollution, and unsustainable practices have degraded the most critical coastal ecosystems including coral reefs, oyster reefs, and salt marshes. Conventional restoration methods work only under perfect conditions, but fail nearly completely when the water becomes too hot or water quality deteriorates. New methods are needed to greatly increase settlement, growth, survival, and resistance to environmental stress of keystone marine organisms in order to maintain critical coastal ecosystem functions including shore protection, fisheries, and biodiversity. Electrolysis methods have been applied to marine ecosystem restoration since 1976, with spectacular results (Figures 1(a)-(c)). This paper provides the first overall review of the data. Low-voltage direct current trickle charges are found to increase the settlement of corals 25.86 times higher than uncharged control sites, to increase the mean growth rates of reef-building corals, soft corals, oysters, and salt marsh grass— an average of 3.17 times faster than controls (ranging from 2 to 10 times depending on species and conditions), and to increase the survival of electrically charged marine organisms—an average of 3.47 times greater than controls, with the biggest increases under the most severe environmental stresses. These results are caused by the fundamental biophysical stimulation of natural biochemical energy production pathways, used by all organisms, provided by electrical stimulation under the right conditions. This paper reviews for the first time all published results from properly designed, installed, and maintained projects, and contrasts them with those that do not meet these criteria.展开更多
Vibrio harveyi is a pathogen of various aquatic organisms that has been recently associated with massive mortality episodes in the aquaculture industry.Recurrent outbreaks of vibriosis are closely correlated with the ...Vibrio harveyi is a pathogen of various aquatic organisms that has been recently associated with massive mortality episodes in the aquaculture industry.Recurrent outbreaks of vibriosis are closely correlated with the capacity of this bacterial species to survive long-term starvation conditions.To study the regulation mechanism of gene expression at the transcriptional level in V.harveyi under starvation conditions,the transcriptomic response profiles were determined of the Portunus trituberculatus pathogen V.harveyi strain DY1 under normal conditions and after four weeks of starvation.A total of 4679 and 4661 genes were expressed in the non-starved and starved cells,respectively.The significantly differentially expressed genes(DEGs)between non-starved and starved groups were identified,in which 255 genes were up-regulated and 411 genes were down-regulated.GO analysis and KEGG enrichment analysis were used to analyze the DEGs and revealed the involvement of these DEGs in many pathways,including ABC transporters,flagellum assembly,and fatty acid metabolism.Several DEGs were randomly selected and their expression levels were confirmed by quantitative real-time PCR(qRT-PCR).This is the first comprehensive transcriptomic analysis of starvation ef fects in V.harveyi.Our findings will facilitate future study on stress adaptation and survival mechanisms of V.harveyi.展开更多
The indigenous population of the North can efficiently preserve health and vital activity, and reproduce new generations under extreme climatic and geographic conditions due to peculiar way of life, mental features, r...The indigenous population of the North can efficiently preserve health and vital activity, and reproduce new generations under extreme climatic and geographic conditions due to peculiar way of life, mental features, rituals, regimes of work and rest, social and cultural traditions. Of great importance are also the clothes which are favorable for keeping heat and self-cleaning, some psycho-therapeutic factors (rituals of shamanism), animal food and vegetables. The phenotype of the adaptation reserves of an organism, northern type of metabolism, dominance of the right brain hemisphere play a great role, so does the adaptation of national mentality to the harmony of inner human state with the extreme environmental conditions of high latitudes.展开更多
文摘Increasing stress from global warming, sea level rise, acidification, sedimentation, pollution, and unsustainable practices have degraded the most critical coastal ecosystems including coral reefs, oyster reefs, and salt marshes. Conventional restoration methods work only under perfect conditions, but fail nearly completely when the water becomes too hot or water quality deteriorates. New methods are needed to greatly increase settlement, growth, survival, and resistance to environmental stress of keystone marine organisms in order to maintain critical coastal ecosystem functions including shore protection, fisheries, and biodiversity. Electrolysis methods have been applied to marine ecosystem restoration since 1976, with spectacular results (Figures 1(a)-(c)). This paper provides the first overall review of the data. Low-voltage direct current trickle charges are found to increase the settlement of corals 25.86 times higher than uncharged control sites, to increase the mean growth rates of reef-building corals, soft corals, oysters, and salt marsh grass— an average of 3.17 times faster than controls (ranging from 2 to 10 times depending on species and conditions), and to increase the survival of electrically charged marine organisms—an average of 3.47 times greater than controls, with the biggest increases under the most severe environmental stresses. These results are caused by the fundamental biophysical stimulation of natural biochemical energy production pathways, used by all organisms, provided by electrical stimulation under the right conditions. This paper reviews for the first time all published results from properly designed, installed, and maintained projects, and contrasts them with those that do not meet these criteria.
基金Supported by the Jiangsu Agricultural Industry Technology System(No.02)the National Natural Science Foundation of China(No.31972830)+3 种基金the Natural Science Foundation of Jiangsu Province(No.BK20180915)the Jiangsu Agriculture Science and Technology Innovation Fund(No.CX(18)2012)the Fishery Science and Technology Innovation Projects of Jiangsu Province(No.D2017-3)the “Blue Project” of Yangzhou University and the Projects of Shuichan Sanxin of Jiangsu Province(No.Y2016-33,D2017-3)
文摘Vibrio harveyi is a pathogen of various aquatic organisms that has been recently associated with massive mortality episodes in the aquaculture industry.Recurrent outbreaks of vibriosis are closely correlated with the capacity of this bacterial species to survive long-term starvation conditions.To study the regulation mechanism of gene expression at the transcriptional level in V.harveyi under starvation conditions,the transcriptomic response profiles were determined of the Portunus trituberculatus pathogen V.harveyi strain DY1 under normal conditions and after four weeks of starvation.A total of 4679 and 4661 genes were expressed in the non-starved and starved cells,respectively.The significantly differentially expressed genes(DEGs)between non-starved and starved groups were identified,in which 255 genes were up-regulated and 411 genes were down-regulated.GO analysis and KEGG enrichment analysis were used to analyze the DEGs and revealed the involvement of these DEGs in many pathways,including ABC transporters,flagellum assembly,and fatty acid metabolism.Several DEGs were randomly selected and their expression levels were confirmed by quantitative real-time PCR(qRT-PCR).This is the first comprehensive transcriptomic analysis of starvation ef fects in V.harveyi.Our findings will facilitate future study on stress adaptation and survival mechanisms of V.harveyi.
文摘The indigenous population of the North can efficiently preserve health and vital activity, and reproduce new generations under extreme climatic and geographic conditions due to peculiar way of life, mental features, rituals, regimes of work and rest, social and cultural traditions. Of great importance are also the clothes which are favorable for keeping heat and self-cleaning, some psycho-therapeutic factors (rituals of shamanism), animal food and vegetables. The phenotype of the adaptation reserves of an organism, northern type of metabolism, dominance of the right brain hemisphere play a great role, so does the adaptation of national mentality to the harmony of inner human state with the extreme environmental conditions of high latitudes.
