Aim To synthesize dutasteride. Methods The target compound was synthesized from pregnenolol via eight steps, including esterification, oxidation, hydrolysis, then oxidative ring-opening, cyclization, reduction, oxidiz...Aim To synthesize dutasteride. Methods The target compound was synthesized from pregnenolol via eight steps, including esterification, oxidation, hydrolysis, then oxidative ring-opening, cyclization, reduction, oxidization and finally acylation. Results The structure of the target molecule was identified by ^1H NMR, ^13C NMR and element analysis. The overall yield was 31.5%. Conclusion The effects of different reaction conditions on the yield of product in each step have been investigated and the optimal reaction conditions have been established.展开更多
目的探讨类固醇5α-还原酶2型缺乏症(SRD5A2)的临床特点、基因突变特征。方法回顾分析1例以外阴异常为初诊表现的SRD5A2患儿的临床资料。结果患儿,2岁5个月,社会性别为女性。基础促黄体生成素(LH)0.07 m IU/mL、促卵泡激素(FSH)0.39 m I...目的探讨类固醇5α-还原酶2型缺乏症(SRD5A2)的临床特点、基因突变特征。方法回顾分析1例以外阴异常为初诊表现的SRD5A2患儿的临床资料。结果患儿,2岁5个月,社会性别为女性。基础促黄体生成素(LH)0.07 m IU/mL、促卵泡激素(FSH)0.39 m IU/mL;人绒毛膜促性腺激素(HCG)刺激试验前后,睾酮分别是0.06 ng/mL、3.65ng/mL,双氢睾酮(DHT)分别是19.67 pg/mL、68.25 pg/mL;17-羟孕酮(17-OHP)1.20 ng/mL,雄烯二酮(A 2)0.07 ng/mL;HCG试验后T/DHT为51.72、T/A 2为14.70;抗苗勒管激素(AMH)22.97 ng/mL,抑制素B(INH-B)274.4 pg/mL。盆腔超声和磁共振均未探及子宫及卵巢。染色体为46,XY;性别决定(SRY)基因检测无异常;雄激素受体(AR)基因结果阴性。患儿及父母外周血均检测到2型5α-还原酶(SRD5A2)基因的致病性突变。应用2.5%DHT凝胶涂抹阴茎4个月后阴茎增长2 cm。结论 SRD5A2的诊断以HCG试验后T/DHT增高为主要依据,检测到致病性的SRD5A2基因突变可确诊。展开更多
文摘Aim To synthesize dutasteride. Methods The target compound was synthesized from pregnenolol via eight steps, including esterification, oxidation, hydrolysis, then oxidative ring-opening, cyclization, reduction, oxidization and finally acylation. Results The structure of the target molecule was identified by ^1H NMR, ^13C NMR and element analysis. The overall yield was 31.5%. Conclusion The effects of different reaction conditions on the yield of product in each step have been investigated and the optimal reaction conditions have been established.
