Background:The diversity of resource acquisition strategies of plant roots determines the species coexistence patterns to a certain extent.However,few root physiological traits have been investigated,such as root phos...Background:The diversity of resource acquisition strategies of plant roots determines the species coexistence patterns to a certain extent.However,few root physiological traits have been investigated,such as root phosphatase activity(PA)that affects plant phosphorus(P)uptake.Methods:Root PA and classical root functional traits were investigated for 21 coexisting species in a deciduous broad-leaved forest in warm temperate-subtropical transition zone,China.We analyzed the root order variation of absorptive fine root PA,clarified the attribution of root PA in root economic space(RES)and the different P acquisition strategies of co-occurring species based on the multidimensional RES theory,and determined the dominant factors affecting interspecific variation in root PA.Results:There was no distinct pattern of PA variation with root order in the first three root orders of absorptive fine roots,and root PA was constrained by phylogeny.Root PA is a competitive trait affiliated with the conservation gradient in RES.The tight linkages among root PA,mycorrhizal colonization,diameter,specific root length,and nitrogen concentration suggested trade-offs among P acquisition strategies of co-occurring species,i.e.species with long and fine roots acquire inorganic P by actively exploring the soil and secreting phosphatase to mineralize and hydrolyze organic P,while species with short and thick roots obtain P mainly by investing C in mycorrhizal partners.Conclusions:Collectively,our study provides an insight into the forest species coexistence in climatic transition zones,i.e.species coexistence mechanisms based on diverse phosphorus acquisition strategies.展开更多
We study the structure of arbitrary split Leibniz triple systems with a coherent O-root space. By developing techniques of connections of roots for this kind of triple systems, under certain conditions, in the case of...We study the structure of arbitrary split Leibniz triple systems with a coherent O-root space. By developing techniques of connections of roots for this kind of triple systems, under certain conditions, in the case of T being of maximal length＇, the simplicity of the Leibniz triple systems is characterized.展开更多
Cotton (Gossypium hirsutum L.) is an importantfiber cash crop,but its root traits related to phosphorus (P) acquisition,including mycorrhizal root traits,are poorly understood.Eight cotton varieties bred in northweste...Cotton (Gossypium hirsutum L.) is an importantfiber cash crop,but its root traits related to phosphorus (P) acquisition,including mycorrhizal root traits,are poorly understood.Eight cotton varieties bred in northwestern China that were released between 1950 and 2013 were grown in pots with or without one arbuscular mycorrhizal fungal (AMF) species(Funneliformis mosseae) at three P supply levels (0,50 and 300 mg P as KH_(2)PO_(4)kg^(-1)).Eleven root traits were measured and calculated after 7 wk of growth.The more recent accessions had smaller root diameters,acquired less P and produced less biomass,indicating an (inadvertent) varietal selection for thinner roots that provided less cortical space for AMF,which then increased the need for a high P fertilizer level.At the two lower P levels,the mycorrhizal plants acquired more P and produced more biomass than non-mycorrhizal plants (3.2 vs.0.9 mg P per plant;1.8 vs.0.9 g biomass per plant at P_(0);14.5 vs.1.7 mg P per plant;and 4.7 vs.1.6 g biomass per plant at P_(50)).At the highest P level,the mycorrhizal plants acquired more P than non-mycorrhizal plants (18.8 vs.13.4 mg per P plant),but there was no difference in biomass (6.2 vs.6.3 g per plant).At the intermediate P level,root diameter was significantly positively correlated with shoot biomass,P concentration and the P content of mycorrhizal plants.The results of our study support the importance of the outsourcing model of P acquisition in the root economics space framework.Inadvertent varietal selection in the last decades,resulting in thinner roots and a lower benefit from AMF,has led to a lower productivity of cotton varieties at moderate P supply (i.e.,when mycorrhizal,the average biomass of older varieties 5.0 g per plant vs.biomass of newer varieties 4.4 g per plant),indicating the need to rethink cotton breeding efforts in order to achieve high yields without very high P input.One feasible way to solve the problem of inadvertent varietal selection for cotton is to be aware of the trade-offs between the r展开更多
以"硕丰688"为试材,设置基质槽栽和袋栽2种栽培方式,10.0、7.5、5.0、2.5 L 4种根系基质容积,研究了不同栽培方式和根系空间大小对日光温室番茄生长、产量及品质的影响。结果表明,相同栽培方式下,随着根系空间减小,番茄植株...以"硕丰688"为试材,设置基质槽栽和袋栽2种栽培方式,10.0、7.5、5.0、2.5 L 4种根系基质容积,研究了不同栽培方式和根系空间大小对日光温室番茄生长、产量及品质的影响。结果表明,相同栽培方式下,随着根系空间减小,番茄植株生长量、净光合速率及产量逐渐降低,但果实成熟时间提前,品质显著提高;相同根系容积下,与袋栽相比,槽栽番茄单果重及产量较高。综合产量与品质指标,槽式栽培、单株根系空间7.5 L可作为推荐栽培方式。展开更多
The use of hair roots as experimental samples has been a research focus for understanding the effects of spaceflight on astronauts, because it has many advantages, one of which is the fact that hair matrix cells activ...The use of hair roots as experimental samples has been a research focus for understanding the effects of spaceflight on astronauts, because it has many advantages, one of which is the fact that hair matrix cells actively divide in a hair follicle and sensitively reflect the physical conditions of the human body. In 2009, a research program focusing on the analysis of astronauts’ hairs was initiated to examine the effects of long-term spaceflight on the gene expression and mineral metabolism in the human body. Since the number of samples per astronaut is limited to 5 strands of hairs at each sampling point, due to the ethical viewpoint of astronauts or limited resources in space, it is important to develop an effective method for the molecular analysis of small amounts of hair roots. In this study, mRNA successfully extracted from 1, 5, and 10 hair follicles was amplified and subjected to the DNA microarray analysis to compare the gene expression within subjects. The results indicated that (1) it was possible to perform the genetic analysis on hair samples stored at -80℃, even without a fixation buffer and (2) the newly modified method of mRNA extraction and analysis was effective in detecting differential gene expression in samples containing only 5 hairs. In conclusion, RNA was efficiently extracted from 5 hair roots, which is the same number of hair roots used in the space experiment;therefore, this method can be applied to genetically analyze astronauts’ hair samples.展开更多
An image trust root is a special type of soft trust root for trusted computing. However,image trust root generation is difficult,as it needs a corresponding stable logic feature generation model and algorithm for dyna...An image trust root is a special type of soft trust root for trusted computing. However,image trust root generation is difficult,as it needs a corresponding stable logic feature generation model and algorithm for dynamical and sustained authentication. This paper proposes a basic function of constructing new scale-spaces with deep detecting ability and high stability for image features aimed at image root generation. According to the heat distribution and spreading principle of various kinds of infinitesimal heat sources in the space medium,a multi-embed nonlinear diffusion equation that corresponds to the multi-embed nonlinear scale-space is proposed,a HARRIS-HESSIAN scale-space evaluation operator that aims at the structure acceleration characteristics of a local region and can make use of image pixels' relative spreading movement principle was constructed,then a single-parameter global symmetric proportion(SPGSP) operator was also constructed. An authentication test with 3000 to 5000 cloud entities shows the new scale-space can work well and is stable,when the whole cloud has 5%-50% behavior with un-trusted entities. Consequently,it can be used as the corresponding stable logic feature generation model and algorithm for all kinds of images,and logic relationships among image features for trust roots.展开更多
基金financially supported by the National Natural Science Foundation of China(Nos.32230067,32001171 and 32001120)。
文摘Background:The diversity of resource acquisition strategies of plant roots determines the species coexistence patterns to a certain extent.However,few root physiological traits have been investigated,such as root phosphatase activity(PA)that affects plant phosphorus(P)uptake.Methods:Root PA and classical root functional traits were investigated for 21 coexisting species in a deciduous broad-leaved forest in warm temperate-subtropical transition zone,China.We analyzed the root order variation of absorptive fine root PA,clarified the attribution of root PA in root economic space(RES)and the different P acquisition strategies of co-occurring species based on the multidimensional RES theory,and determined the dominant factors affecting interspecific variation in root PA.Results:There was no distinct pattern of PA variation with root order in the first three root orders of absorptive fine roots,and root PA was constrained by phylogeny.Root PA is a competitive trait affiliated with the conservation gradient in RES.The tight linkages among root PA,mycorrhizal colonization,diameter,specific root length,and nitrogen concentration suggested trade-offs among P acquisition strategies of co-occurring species,i.e.species with long and fine roots acquire inorganic P by actively exploring the soil and secreting phosphatase to mineralize and hydrolyze organic P,while species with short and thick roots obtain P mainly by investing C in mycorrhizal partners.Conclusions:Collectively,our study provides an insight into the forest species coexistence in climatic transition zones,i.e.species coexistence mechanisms based on diverse phosphorus acquisition strategies.
基金Supported by Scientific Research Fund of Heilongjiang Provincial Education Department(Grant No.12541184)
文摘We study the structure of arbitrary split Leibniz triple systems with a coherent O-root space. By developing techniques of connections of roots for this kind of triple systems, under certain conditions, in the case of T being of maximal length＇, the simplicity of the Leibniz triple systems is characterized.
基金financially supported by the National Natural Science Foundation of China(32272807 and U1703232)supported via project from State Key Laboratory of North China Crop Improvement and Regulation(NCCIR2021ZZ-1)。
文摘Cotton (Gossypium hirsutum L.) is an importantfiber cash crop,but its root traits related to phosphorus (P) acquisition,including mycorrhizal root traits,are poorly understood.Eight cotton varieties bred in northwestern China that were released between 1950 and 2013 were grown in pots with or without one arbuscular mycorrhizal fungal (AMF) species(Funneliformis mosseae) at three P supply levels (0,50 and 300 mg P as KH_(2)PO_(4)kg^(-1)).Eleven root traits were measured and calculated after 7 wk of growth.The more recent accessions had smaller root diameters,acquired less P and produced less biomass,indicating an (inadvertent) varietal selection for thinner roots that provided less cortical space for AMF,which then increased the need for a high P fertilizer level.At the two lower P levels,the mycorrhizal plants acquired more P and produced more biomass than non-mycorrhizal plants (3.2 vs.0.9 mg P per plant;1.8 vs.0.9 g biomass per plant at P_(0);14.5 vs.1.7 mg P per plant;and 4.7 vs.1.6 g biomass per plant at P_(50)).At the highest P level,the mycorrhizal plants acquired more P than non-mycorrhizal plants (18.8 vs.13.4 mg per P plant),but there was no difference in biomass (6.2 vs.6.3 g per plant).At the intermediate P level,root diameter was significantly positively correlated with shoot biomass,P concentration and the P content of mycorrhizal plants.The results of our study support the importance of the outsourcing model of P acquisition in the root economics space framework.Inadvertent varietal selection in the last decades,resulting in thinner roots and a lower benefit from AMF,has led to a lower productivity of cotton varieties at moderate P supply (i.e.,when mycorrhizal,the average biomass of older varieties 5.0 g per plant vs.biomass of newer varieties 4.4 g per plant),indicating the need to rethink cotton breeding efforts in order to achieve high yields without very high P input.One feasible way to solve the problem of inadvertent varietal selection for cotton is to be aware of the trade-offs between the r
基金Supported by NNSF of China (11801121)NSF of Heilongjiang province(QC2018006)the Fundamental Research Fundation for Universities of Heilongjiang Province(LGYC2018JC002)。
文摘以"硕丰688"为试材,设置基质槽栽和袋栽2种栽培方式,10.0、7.5、5.0、2.5 L 4种根系基质容积,研究了不同栽培方式和根系空间大小对日光温室番茄生长、产量及品质的影响。结果表明,相同栽培方式下,随着根系空间减小,番茄植株生长量、净光合速率及产量逐渐降低,但果实成熟时间提前,品质显著提高;相同根系容积下,与袋栽相比,槽栽番茄单果重及产量较高。综合产量与品质指标,槽式栽培、单株根系空间7.5 L可作为推荐栽培方式。
基金Supported by National Natural Science Foundation of China(11801121)NSF of Heilongjiang Province(QC2018006)the Fundamental Research Fundation for Universities of Heilongjiang Province(LGYC2018JC002)。
文摘The use of hair roots as experimental samples has been a research focus for understanding the effects of spaceflight on astronauts, because it has many advantages, one of which is the fact that hair matrix cells actively divide in a hair follicle and sensitively reflect the physical conditions of the human body. In 2009, a research program focusing on the analysis of astronauts’ hairs was initiated to examine the effects of long-term spaceflight on the gene expression and mineral metabolism in the human body. Since the number of samples per astronaut is limited to 5 strands of hairs at each sampling point, due to the ethical viewpoint of astronauts or limited resources in space, it is important to develop an effective method for the molecular analysis of small amounts of hair roots. In this study, mRNA successfully extracted from 1, 5, and 10 hair follicles was amplified and subjected to the DNA microarray analysis to compare the gene expression within subjects. The results indicated that (1) it was possible to perform the genetic analysis on hair samples stored at -80℃, even without a fixation buffer and (2) the newly modified method of mRNA extraction and analysis was effective in detecting differential gene expression in samples containing only 5 hairs. In conclusion, RNA was efficiently extracted from 5 hair roots, which is the same number of hair roots used in the space experiment;therefore, this method can be applied to genetically analyze astronauts’ hair samples.
基金The national natural science foundation (61672442,61503316,61273290,61373147)Xiamen Scientific Plan Project (2014S0048,3502Z20123037)+1 种基金Fujian Scientific Plan Project (2013HZ00041)Fujian provincial education office A-class project(JA13238)
文摘An image trust root is a special type of soft trust root for trusted computing. However,image trust root generation is difficult,as it needs a corresponding stable logic feature generation model and algorithm for dynamical and sustained authentication. This paper proposes a basic function of constructing new scale-spaces with deep detecting ability and high stability for image features aimed at image root generation. According to the heat distribution and spreading principle of various kinds of infinitesimal heat sources in the space medium,a multi-embed nonlinear diffusion equation that corresponds to the multi-embed nonlinear scale-space is proposed,a HARRIS-HESSIAN scale-space evaluation operator that aims at the structure acceleration characteristics of a local region and can make use of image pixels' relative spreading movement principle was constructed,then a single-parameter global symmetric proportion(SPGSP) operator was also constructed. An authentication test with 3000 to 5000 cloud entities shows the new scale-space can work well and is stable,when the whole cloud has 5%-50% behavior with un-trusted entities. Consequently,it can be used as the corresponding stable logic feature generation model and algorithm for all kinds of images,and logic relationships among image features for trust roots.
