Synthetic biology aims to design and build new biological systems with desirable properties,providing the foundation for the biosynthesis of secondary metabolites.The most prominent representation of synthetic biology...Synthetic biology aims to design and build new biological systems with desirable properties,providing the foundation for the biosynthesis of secondary metabolites.The most prominent representation of synthetic biology has been used in microbial engineering by recombinant DNA technology.However,there are advantages of using a deleted host,and therefore an increasing number of biotechnology studies follow similar strategies to dissect cellular networks and construct genomereduced microbes.This review will give an overview of the strategies used for constructing and engineering reduced-genome factories by synthetic biology to improve production of secondary metabolites.展开更多
苦草(Vallisneria)隶属于水鳖科(Hydrocharitaceae),是水域生态修复的先锋物种。为了对苦草属植物进行比较全面的SSR信息分析,本研究基于RAD-seq (restriction-site associated DNA sequencing)简化基因组测序技术获得了苦草的简化基因...苦草(Vallisneria)隶属于水鳖科(Hydrocharitaceae),是水域生态修复的先锋物种。为了对苦草属植物进行比较全面的SSR信息分析,本研究基于RAD-seq (restriction-site associated DNA sequencing)简化基因组测序技术获得了苦草的简化基因组序列信息,并开发了SSR分子标记与引物设计。在所得序列中共检测到366个SSR位点,成功设计出引物的有355个,其中二碱基重复基序数量最多,占总数的59.56%,通过筛选和验证,最终确定了23对具有多态性的SSR引物。Genepop软件分析结果显示,这23个位点的等位基因数均值为3.26,多态性高且不连锁(P<0.05);4个位点在多数群体中偏离哈迪温伯格平衡(P<0.01)且纯合子与杂合子比例相当(观测杂合度均值0.460),近交系数高(Fis均值0.880),可能是因为单个群体采样集中,且由于苦草属植物同时具备无性、有性两种繁殖方式中的无性繁殖所致。展开更多
基金supported in part by grants from the National Programs for High Technology Research and Development Program(863 Program)(Grant Nos.2006AA09Z402,2007AA09Z443)the National Basic Research Program of China(973 Program)(Grant No.2004CB719601)+5 种基金National Natural Science Foundation of China(Grant Nos.30560001,30600001,30700015)National Key Technology R&D Program(2007BAI26B02)the National Science&Technology Pillar Program(No.200703295000-02)Important National Science&Technology Specific Projects(Nos.2008ZX09401-005,2009ZX09302-004)Science and Technology Planning Project of Guangdong Province,China(No.2006A50103001)Key Project of International Cooperation(2007DFB31620)。
文摘Synthetic biology aims to design and build new biological systems with desirable properties,providing the foundation for the biosynthesis of secondary metabolites.The most prominent representation of synthetic biology has been used in microbial engineering by recombinant DNA technology.However,there are advantages of using a deleted host,and therefore an increasing number of biotechnology studies follow similar strategies to dissect cellular networks and construct genomereduced microbes.This review will give an overview of the strategies used for constructing and engineering reduced-genome factories by synthetic biology to improve production of secondary metabolites.
文摘苦草(Vallisneria)隶属于水鳖科(Hydrocharitaceae),是水域生态修复的先锋物种。为了对苦草属植物进行比较全面的SSR信息分析,本研究基于RAD-seq (restriction-site associated DNA sequencing)简化基因组测序技术获得了苦草的简化基因组序列信息,并开发了SSR分子标记与引物设计。在所得序列中共检测到366个SSR位点,成功设计出引物的有355个,其中二碱基重复基序数量最多,占总数的59.56%,通过筛选和验证,最终确定了23对具有多态性的SSR引物。Genepop软件分析结果显示,这23个位点的等位基因数均值为3.26,多态性高且不连锁(P<0.05);4个位点在多数群体中偏离哈迪温伯格平衡(P<0.01)且纯合子与杂合子比例相当(观测杂合度均值0.460),近交系数高(Fis均值0.880),可能是因为单个群体采样集中,且由于苦草属植物同时具备无性、有性两种繁殖方式中的无性繁殖所致。
