摘要
大肠杆菌是遗传重组领域广泛应用的宿主之一,用于生产重组蛋白、氨基酸和其他化学品。基因组减小可以减少代谢调节网络中的冗余,提高其预测性和可控性。我们介绍了最小基因组的研究策略、应用无痕敲除技术减小大肠杆菌基因组的方法,以及基因组减小后对菌体生长特性、附加体稳定性、重组蛋白表达和代谢的影响。
Escherichia coli is one of the best hosts for genetic recombination and has been used in the pharmaceutical and fermentation industries to make recombinant proteins, amino aicds and other chemicals. Genome size reduction may decrease the redundancy of regulatory and metabolic networks, and provide a more predictable and controllable system than the wild type strain. In this paper we reviewed the research tactics for a minimum genome, the methods of reducing genome by markerless deletion, the growth characteristics of the reduced-genome strain and its effect on the plasmid stability, the recombinant protein expression and the metabolism.
出处
《生物技术通讯》
CAS
2009年第4期564-567,共4页
Letters in Biotechnology
关键词
大肠杆菌
无痕敲除
基因组减小
最小基因组
Escherichia coli
markerless deletion
reduced genome
minimum genome
