摘要
采用近红外方法,分析104份甘蓝型油菜种子中的芥酸、油酸与硫代葡萄糖苷含量;运用简化基因组技术(ddRADseq)对全部材料进行基因分型;结合性状与基因型的数据,利用Tassel混合线性模型进行全基因组关联分析。结果表明:芥酸含量与硫代葡萄糖苷呈极显著正相关,油酸含量与芥酸、硫代葡萄糖苷呈极显著负相关;群体结构分析将104份材料划分为4个亚群;通过全基因组关联分析发现,与芥酸、油酸含量显著关联的SNP标记主要分布在A08与C03染色体上,与硫代葡萄糖苷含量显著关联的标记分布在A05、A02与C09染色体上。
Brassica napus is the main oil crop and the main source of edible vegetable oil in China. High-quality "double low" rapeseed is an important goal for the genetic breeding of Brassica napus. In this study, the content of erucic acid, oleic acid, and glucosinolate in 104 Brassica napus L. seeds has been analyzed by near-infrared method, and all materials have been genotyped by simplified genome technology(ddRADseq). Combining traits with genotype data, a Tassel mixed linear model has been used for genome-wide association analysis. The results showed that there was a significant positive correlation between erucic acid content and glucosinolates, and a significant negative correlation between oleic acid content and erucic acid and glucosinolates. The population structure analysis divided 104 materials into 4 subgroups. It was found that SNP markers significantly associated with erucic acid and the oleic acid content were mainly distributed on the A08 and C03 chromosomes, and markers associated with glucosinolate content were distributed on chromosomes A05, A02 and C09.
作者
陈聪
刘忠松
CHEN Cong;LIU Zhongsong(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第2期138-143,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学基金项目(3157101920)。
关键词
甘蓝型油菜
全基因组关联分析
简化基因组测序
群体结构
品质性状
Brassica napus
genome-wide association analysis
reduced-representation genome sequencing
population structure
quality-related traits
