This work presents the pathology description, isolation and identification of canine herpesvirus (CHV-1) in Mexico, a virus that causes a generalized hemorrhagic infection in puppies from the canidae family. Methods: ...This work presents the pathology description, isolation and identification of canine herpesvirus (CHV-1) in Mexico, a virus that causes a generalized hemorrhagic infection in puppies from the canidae family. Methods: Isolates were obtained from puppies that died within the first four weeks of life and had lesions consistent with canine herpesvirus. Results: The main gross lesions were petechial and ecchymotic hemorrhages in kidneys, liver and lungs;proliferative interstitial nephritis;multifocal necrosis in liver and kidneys;and encephalitis with intranuclear inclusion bodies. Herpesvirus was confirmed through direct immunofluorescence, electron microscopy and polymerase chain reaction for DNA polymerase and glycoprotein B genes. Discussion: Eight strains were isolated and identified as canine herpesvirus corresponding to three of the working cases with gross and microscopic lesions very similar to those described in the literature;then, isolates were confirmed by PCR gene amplification, positive reactions on immunofluorescence and observations from electron microscopy. This work represents the first report of this disease, including gross and histological lesions, and confirmation by isolation and identification of the canine herpesvirus in Mexico.展开更多
The experiment was to study the effect of glutamine on serum GPT and AKP activity in the puppies. Sixteen 60 day-old Germany Shepherd puppies[weight of （5.86±0.22） kg] were selected and randomly divided into 2 ...The experiment was to study the effect of glutamine on serum GPT and AKP activity in the puppies. Sixteen 60 day-old Germany Shepherd puppies[weight of （5.86±0.22） kg] were selected and randomly divided into 2 groups, one group fed basal diet was the control group, another group fed basal diet and supplemented with 1.2% glutamine was the experimental group. Trial period was 21 days. GPT and AKP activity were detected on 7th, 14th and 21 st day respectively. Dietary sup- plementation with 1.2% glutamine enhanced （P〈0.05） its activity of serum GPT and AKP compared with the control group. This experiment proved that glutamine could improve these two kinds of enzyme activity in serum, promotes protein synthesis, raise puppies anti-stress effect.展开更多
文摘This work presents the pathology description, isolation and identification of canine herpesvirus (CHV-1) in Mexico, a virus that causes a generalized hemorrhagic infection in puppies from the canidae family. Methods: Isolates were obtained from puppies that died within the first four weeks of life and had lesions consistent with canine herpesvirus. Results: The main gross lesions were petechial and ecchymotic hemorrhages in kidneys, liver and lungs;proliferative interstitial nephritis;multifocal necrosis in liver and kidneys;and encephalitis with intranuclear inclusion bodies. Herpesvirus was confirmed through direct immunofluorescence, electron microscopy and polymerase chain reaction for DNA polymerase and glycoprotein B genes. Discussion: Eight strains were isolated and identified as canine herpesvirus corresponding to three of the working cases with gross and microscopic lesions very similar to those described in the literature;then, isolates were confirmed by PCR gene amplification, positive reactions on immunofluorescence and observations from electron microscopy. This work represents the first report of this disease, including gross and histological lesions, and confirmation by isolation and identification of the canine herpesvirus in Mexico.
文摘The experiment was to study the effect of glutamine on serum GPT and AKP activity in the puppies. Sixteen 60 day-old Germany Shepherd puppies[weight of （5.86±0.22） kg] were selected and randomly divided into 2 groups, one group fed basal diet was the control group, another group fed basal diet and supplemented with 1.2% glutamine was the experimental group. Trial period was 21 days. GPT and AKP activity were detected on 7th, 14th and 21 st day respectively. Dietary sup- plementation with 1.2% glutamine enhanced （P〈0.05） its activity of serum GPT and AKP compared with the control group. This experiment proved that glutamine could improve these two kinds of enzyme activity in serum, promotes protein synthesis, raise puppies anti-stress effect.
