The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt is reported. It was synthesized by coupling Phac-Met-OEt with Gly-OMe ·HC1, Trp-OMe and Met-OEt successively, catalyzed by α-chymotrypsin, papai...The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt is reported. It was synthesized by coupling Phac-Met-OEt with Gly-OMe ·HC1, Trp-OMe and Met-OEt successively, catalyzed by α-chymotrypsin, papain and α-chymotrypsin respectively. The results of FAB-MS showed that the products had the correct molecular mass.展开更多
The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, T...The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-OMe and Met-OEt successively These three steps were catalyzed by α-chymotrpsin, Papain and α-chymotrpsin respectively The results of FAB-MS showed that all the products had the correct molecular mass展开更多
目的分析与探讨微孔板木瓜酶法和抗人球蛋白微柱凝胶法联合检测献血者不规则抗体的具体情况。方法将收集到的12293例无偿献血者标本进行微孔板木瓜酶法的抗体筛查,筛查到阳性标本联合抗人球蛋白微柱凝胶法检测,将检测为阳性的标本送输...目的分析与探讨微孔板木瓜酶法和抗人球蛋白微柱凝胶法联合检测献血者不规则抗体的具体情况。方法将收集到的12293例无偿献血者标本进行微孔板木瓜酶法的抗体筛查,筛查到阳性标本联合抗人球蛋白微柱凝胶法检测,将检测为阳性的标本送输血研究室确认和鉴定,并计算微孔板木瓜酶法和抗人球蛋白微柱凝胶法联合检测的耗时和成本。结果12293例标本中微孔板木瓜酶法筛查到阳性标本140例,阳性率为11.39‰,联合微柱凝胶法检测到6例阳性标本,阳性率为0.49‰。判定为阳性的标本送输血研究室鉴定,IgG型3例(抗-E型1例,未知型抗体2例),IgM型3例(抗-Lea型、抗-Leb型和抗-Lea+Leb型各1例)。标本数为0~88个(一批次)时联合检测耗时为1 h 45 min~1 h 50 min,352个标本(四批次)以内每增加0~88个的标本(一批次)耗时增加约10 min。检测成本约为每个标本0.37元。结论利用微孔板木瓜酶法和微柱凝胶法联合筛查能筛检出常规血型仪检测不出的不规则抗体,且此方法灵敏度高、特异度强、检测耗时短、成本低,适合血站系统对献血者不规则抗体的大规模筛查,值得推广。展开更多
文摘The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt is reported. It was synthesized by coupling Phac-Met-OEt with Gly-OMe ·HC1, Trp-OMe and Met-OEt successively, catalyzed by α-chymotrypsin, papain and α-chymotrypsin respectively. The results of FAB-MS showed that the products had the correct molecular mass.
文摘The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-OMe and Met-OEt successively These three steps were catalyzed by α-chymotrpsin, Papain and α-chymotrpsin respectively The results of FAB-MS showed that all the products had the correct molecular mass
文摘目的分析与探讨微孔板木瓜酶法和抗人球蛋白微柱凝胶法联合检测献血者不规则抗体的具体情况。方法将收集到的12293例无偿献血者标本进行微孔板木瓜酶法的抗体筛查,筛查到阳性标本联合抗人球蛋白微柱凝胶法检测,将检测为阳性的标本送输血研究室确认和鉴定,并计算微孔板木瓜酶法和抗人球蛋白微柱凝胶法联合检测的耗时和成本。结果12293例标本中微孔板木瓜酶法筛查到阳性标本140例,阳性率为11.39‰,联合微柱凝胶法检测到6例阳性标本,阳性率为0.49‰。判定为阳性的标本送输血研究室鉴定,IgG型3例(抗-E型1例,未知型抗体2例),IgM型3例(抗-Lea型、抗-Leb型和抗-Lea+Leb型各1例)。标本数为0~88个(一批次)时联合检测耗时为1 h 45 min~1 h 50 min,352个标本(四批次)以内每增加0~88个的标本(一批次)耗时增加约10 min。检测成本约为每个标本0.37元。结论利用微孔板木瓜酶法和微柱凝胶法联合筛查能筛检出常规血型仪检测不出的不规则抗体,且此方法灵敏度高、特异度强、检测耗时短、成本低,适合血站系统对献血者不规则抗体的大规模筛查,值得推广。
