Objective:Andrographis paniculata is a well-known medicinal plant in Southeast Asia,India and China.The plant contains andrographolide(AN),a very important phytochemical used in various health problems.However,AN is l...Objective:Andrographis paniculata is a well-known medicinal plant in Southeast Asia,India and China.The plant contains andrographolide(AN),a very important phytochemical used in various health problems.However,AN is low in oral absorption bioavailability of AN due to the rapid clearance and high protein binding capacity.Methods:The present study was aimed to develop a nano-phytovesicular formulation of semi-purified AN extracts from a naturally occurring phospholipid(soya phosphatidylcholine)in order to increase the oral absorption and antihyperglycemic activity in rats.Results:The nano-phyto vesicle of semi-purified AN extracts equivalent to 25 mg/kg AN significantly protected the hyperglycemic condition of rats.The in vitro and in vivo experiments results proved that the nano-phytovesicular system of plant extracts containing AN produced better oral absorption,bioavailability and improved antihyperglycemic activity compared with that of free AN at dose of 50 mg/kg.Conclusion:Hence,the prepared semi-purified extract nano-phytovesicular system is helpful in solving the problem of rapid clearance of AN.展开更多
目的:建立测定穿心莲叶中常用的4种农药残留量的方法,更好地控制药材的质量。方法:以灭蚜威为测定内标,样品经乙腈提取,采用Atlantics d C18柱分离,液相色谱-串联质谱联用(LC—MS/MS)方法测定穿心莲药材中常用的4种农药残留量。结果:4...目的:建立测定穿心莲叶中常用的4种农药残留量的方法,更好地控制药材的质量。方法:以灭蚜威为测定内标,样品经乙腈提取,采用Atlantics d C18柱分离,液相色谱-串联质谱联用(LC—MS/MS)方法测定穿心莲药材中常用的4种农药残留量。结果:4种农药检测限在0.003～0.0093 mg·kg-1之间,回收率在83%～112%之间,回收率相对标准偏差均低于6%。结论:方法简便、灵敏、重现性好,能满足同时测定4种农药在穿心莲药材中的残留量。展开更多
基金financial support through UGC-JRF in Engineering and Technology(Vide-F No.10-01/2008 SA-I)。
文摘Objective:Andrographis paniculata is a well-known medicinal plant in Southeast Asia,India and China.The plant contains andrographolide(AN),a very important phytochemical used in various health problems.However,AN is low in oral absorption bioavailability of AN due to the rapid clearance and high protein binding capacity.Methods:The present study was aimed to develop a nano-phytovesicular formulation of semi-purified AN extracts from a naturally occurring phospholipid(soya phosphatidylcholine)in order to increase the oral absorption and antihyperglycemic activity in rats.Results:The nano-phyto vesicle of semi-purified AN extracts equivalent to 25 mg/kg AN significantly protected the hyperglycemic condition of rats.The in vitro and in vivo experiments results proved that the nano-phytovesicular system of plant extracts containing AN produced better oral absorption,bioavailability and improved antihyperglycemic activity compared with that of free AN at dose of 50 mg/kg.Conclusion:Hence,the prepared semi-purified extract nano-phytovesicular system is helpful in solving the problem of rapid clearance of AN.
文摘目的:建立测定穿心莲叶中常用的4种农药残留量的方法,更好地控制药材的质量。方法:以灭蚜威为测定内标,样品经乙腈提取,采用Atlantics d C18柱分离,液相色谱-串联质谱联用(LC—MS/MS)方法测定穿心莲药材中常用的4种农药残留量。结果:4种农药检测限在0.003～0.0093 mg·kg-1之间,回收率在83%～112%之间,回收率相对标准偏差均低于6%。结论:方法简便、灵敏、重现性好,能满足同时测定4种农药在穿心莲药材中的残留量。
