Objectives To evaluate the levels of total testosterone （TT）, free testosterone （FT）, matrix metalloproteinase-9（MMP-9）,and tissue inhibitor of metalloproteinase-1（TIMP-1）, soluble intercellular adhesion molec...Objectives To evaluate the levels of total testosterone （TT）, free testosterone （FT）, matrix metalloproteinase-9（MMP-9）,and tissue inhibitor of metalloproteinase-1（TIMP-1）, soluble intercellular adhesion molecule-1 （sICAM-1）, C-reactive protein （CRP）and association between them in male patients with different type of coronary heart diseases, and to assess the role they played in the pathogenesis of male acute coronary syndrome（ACS）.Methods 90 patients with coronary heart disease （CHD） including acute myocardial infarction （AMI）, unstable angina pectoris（UAP） , stable angina pectoris（SAP）and 30 healthy volunteers as controls were recruited. Serum concentrations of MMP-9, TIMP-1,sICAM-1 were measured with ELISA kits. CRP levels were measured with immunoturbidimetric assay, and white blood cell count （WBC） were also assessed before any treatment was administrated . Results ① The serum levels of free testosterone were significantly lower in patients with ACS （14.41±3.97 pg/mL in AMI group, 17.22±4.05 pg/mL in UAP group, and 26.49±8.72 pg/mL in control group）. MMP-9 levels （257.38±37.14 μg/L in AMI, 147.91±32.47 μg/L in UAP, and 57.26±31.75 μg/L in controls） and the ratios of MMP-9/TIMP-1 （4.23±0.51 in AMI, 2.32±0.38 in UAP, and 1.02±0.32 in controls）, meanwhile, were notably higher in ACS patients than those in control group（P〈 0.01）. ② Significant negative correlation between the serum levels of testosterone and MMP-9（-0.328 for TT, P〈0.05; -0.542 for FT,P 〈 0.01）, and MMP-9/TIMP-1（-0.309 for TT, P〈0.05; -0.519 for FT, P〈0.01） were observed in ACS groups. ③ The serum levels of sICAM-1, CRP and WBC in the patients with ACS were significantly higher than those in the control group （P〈 0.01）. Conclusions The elevation of serum concentrations of inflammatory markers including MMP-9, sICAM-1, CRP and WBC are associated with initiation and progression of ACS, while there are significant negative correlation between th展开更多
目的探讨含血小板结合蛋白基序1的解聚蛋白样金属蛋白酶（a disintegrin and metalloproteinase with thrombospondin type I motif，ADAMTS1）在结直肠癌组织中的表达情况，并分析其与结直肠癌临床病理特征及预后的关系。方法采用免疫...目的探讨含血小板结合蛋白基序1的解聚蛋白样金属蛋白酶（a disintegrin and metalloproteinase with thrombospondin type I motif，ADAMTS1）在结直肠癌组织中的表达情况，并分析其与结直肠癌临床病理特征及预后的关系。方法采用免疫组化染色SP法检测65例结直肠癌组织和癌旁组织中ADAMTS1的表达，采用妒检验分析ADAMTS1表达与结直肠癌临床病理特征的关系，采用Cox比例风险模型分析ADAMTS1表达及其他临床病理特征与结直肠癌预后的关系。结果结直肠癌组织中ADAMTS1的表达阳性率为40％（26／65），癌旁组织为85％（55／65），结直肠癌组织较低（X=27．546，P〈0．001）。有淋巴结转移的结直肠癌组织其ADAMTS1的表达阳性率低于无淋巴结转移者（X=5．329，P=0．021）。ADAMTS1表达阴性结直肠癌患者的中位生存时间为27个月，而ADAMTS1表达阳性者为70个月，ADAMTS1表达阳性者的生存情况较好（X=10．151，P=0．001）。Cox比例风险模型分析结果显示，TNM分期（RR=3．782，95％ CI：1．509-9．476，P=0．005）、淋巴结转移（RR=3．107，95％ CI：1．186-8．138，P=0．021）和ADAMTS1表达情况（RR=2．020，95％ CI：1．071-3．809，P=-0．030）均影响结直肠癌患者的预后，Ⅰ-Ⅱ期、无淋巴结转移及ADAMTSI表达阳性者的预后较好。结论ADAMTS1在结直肠癌组织中呈低表达；ADAMTS1表达与结直肠癌的淋巴结转移有关；ADAMTS1表达阳性结直肠癌患者的预后好于ADAMTS1表达阴性者，提示其是预测结直肠癌患者预后的重要指标。展开更多
文摘Objectives To evaluate the levels of total testosterone （TT）, free testosterone （FT）, matrix metalloproteinase-9（MMP-9）,and tissue inhibitor of metalloproteinase-1（TIMP-1）, soluble intercellular adhesion molecule-1 （sICAM-1）, C-reactive protein （CRP）and association between them in male patients with different type of coronary heart diseases, and to assess the role they played in the pathogenesis of male acute coronary syndrome（ACS）.Methods 90 patients with coronary heart disease （CHD） including acute myocardial infarction （AMI）, unstable angina pectoris（UAP） , stable angina pectoris（SAP）and 30 healthy volunteers as controls were recruited. Serum concentrations of MMP-9, TIMP-1,sICAM-1 were measured with ELISA kits. CRP levels were measured with immunoturbidimetric assay, and white blood cell count （WBC） were also assessed before any treatment was administrated . Results ① The serum levels of free testosterone were significantly lower in patients with ACS （14.41±3.97 pg/mL in AMI group, 17.22±4.05 pg/mL in UAP group, and 26.49±8.72 pg/mL in control group）. MMP-9 levels （257.38±37.14 μg/L in AMI, 147.91±32.47 μg/L in UAP, and 57.26±31.75 μg/L in controls） and the ratios of MMP-9/TIMP-1 （4.23±0.51 in AMI, 2.32±0.38 in UAP, and 1.02±0.32 in controls）, meanwhile, were notably higher in ACS patients than those in control group（P〈 0.01）. ② Significant negative correlation between the serum levels of testosterone and MMP-9（-0.328 for TT, P〈0.05; -0.542 for FT,P 〈 0.01）, and MMP-9/TIMP-1（-0.309 for TT, P〈0.05; -0.519 for FT, P〈0.01） were observed in ACS groups. ③ The serum levels of sICAM-1, CRP and WBC in the patients with ACS were significantly higher than those in the control group （P〈 0.01）. Conclusions The elevation of serum concentrations of inflammatory markers including MMP-9, sICAM-1, CRP and WBC are associated with initiation and progression of ACS, while there are significant negative correlation between th
文摘目的探讨含血小板结合蛋白基序1的解聚蛋白样金属蛋白酶（a disintegrin and metalloproteinase with thrombospondin type I motif，ADAMTS1）在结直肠癌组织中的表达情况，并分析其与结直肠癌临床病理特征及预后的关系。方法采用免疫组化染色SP法检测65例结直肠癌组织和癌旁组织中ADAMTS1的表达，采用妒检验分析ADAMTS1表达与结直肠癌临床病理特征的关系，采用Cox比例风险模型分析ADAMTS1表达及其他临床病理特征与结直肠癌预后的关系。结果结直肠癌组织中ADAMTS1的表达阳性率为40％（26／65），癌旁组织为85％（55／65），结直肠癌组织较低（X=27．546，P〈0．001）。有淋巴结转移的结直肠癌组织其ADAMTS1的表达阳性率低于无淋巴结转移者（X=5．329，P=0．021）。ADAMTS1表达阴性结直肠癌患者的中位生存时间为27个月，而ADAMTS1表达阳性者为70个月，ADAMTS1表达阳性者的生存情况较好（X=10．151，P=0．001）。Cox比例风险模型分析结果显示，TNM分期（RR=3．782，95％ CI：1．509-9．476，P=0．005）、淋巴结转移（RR=3．107，95％ CI：1．186-8．138，P=0．021）和ADAMTS1表达情况（RR=2．020，95％ CI：1．071-3．809，P=-0．030）均影响结直肠癌患者的预后，Ⅰ-Ⅱ期、无淋巴结转移及ADAMTSI表达阳性者的预后较好。结论ADAMTS1在结直肠癌组织中呈低表达；ADAMTS1表达与结直肠癌的淋巴结转移有关；ADAMTS1表达阳性结直肠癌患者的预后好于ADAMTS1表达阴性者，提示其是预测结直肠癌患者预后的重要指标。
