AIM: To study the biological and clinical characteristics of transcription factor forkhead box protein 3 (FOXP3) in hepatocellular carcinoma (HCC). METHODS: We analyzed the expression and localization of FOXP3 in HCC ...AIM: To study the biological and clinical characteristics of transcription factor forkhead box protein 3 (FOXP3) in hepatocellular carcinoma (HCC). METHODS: We analyzed the expression and localization of FOXP3 in HCC tissues and cell lines to evaluate its biological features. The relationship between FOXP3 staining and clinical risk factors of HCC was assessedto identify the clinical characteristics of FOXP3 in HCC. RESULTS: The mRNA and protein expression of FOXP3 were found in some hepatoma cell lines. Immunohistochemical (IHC) analysis of HCC sections revealed that 48% of HCC displayed FOXP3 staining, but we did not find any FOXP3 staining in normal liver tissues and para-tumor tissues. IHC and Confocal analysis showed that the expressions of FOXP3 were mainly present in the nucleus and cytoplasm of tumor cells in tissues or cell lines. In HCC, the distribution of FOXP3 was similar to that of the cirrhosis, but not to the hepatitis B virus. Those findings implicate that FOXP3 staining seems to be associated with the high risk of HCC. CONCLUSION: The clinical characteristics of FOXP3 in HCC warrants further studies to explore its functions and roles in the cirrhosis and development of HCC.展开更多
Background Regulatory T cells （Tregs） are immunologically and clinically interesting not least because of the important role they play in allograft rejection. Likewise, expression of the transcription factor forkhea...Background Regulatory T cells （Tregs） are immunologically and clinically interesting not least because of the important role they play in allograft rejection. Likewise, expression of the transcription factor forkhead box protein 3 （FOXP3）, detected in transplant biopsies, is also of intere.（;t because of its role in the development of regulatory T cells. In this study, we investigated the relationship between FoxP3 mRNA expression and acute organ rejection in kidney-transplant recipients. Methods In this prospective study, FoxP3 mRNA expression levels in peripheral blood samples from 10 recipients of living relative-donor kidney transplants were measured before transplantation as well as at the 14th and 90th days post-transplantation. In addition, 46 first-time kidney-transplant recipients participated in a cross-sectional study, with 28 patients classified as having acute organ rejection; whilst the remaining 18 patients had functionally stable allografts. FoxP3 mRNA expression levels in peripheral blood samples were compared between these two different groups. Results Before transplantation mean FoxP3 mRNA levels vs. GADPH mRNA levels （Ig（FoxP3 mRNA/GADPH mRNA）） in the 10 recipients were 1.11±0.67. The mean FoxP3 mRNA expression levels measured at 14th and 90th days post-transplantation were significantly higher than before transplantation （1.69±0.38, P=0.03; 1.44±0.21, P=0.04, respectively）. Additionally, the mean FoxP3 mRNA levels vs. GADPH mRNA expression levels （Ig（FoxP3 mRNA/GADPH mRNA）） were significantly higher in recipients suffering acute rejection compared with those with stable allografts （1.77±0.61 and 1.43±0.27, respectively, P=0.03）. Conclusions After kidney transplantntion, FoxP3 mRNA levels were found to increase in the peripheral blood of all recipients. Considerably higher FoxP3 rnRNA levels were observed in recipients suffering acute rejection. These results suggest that FoxP3 mRNA levels in peripheral blood samples can be used as a diagnostic tool for i展开更多
传统观点认为,CD4^(+)调节性T细胞(regulatory T cell,Treg)是1类能够感知免疫应答强弱并具有负向调控作用的细胞亚群,在机体免疫自稳及免疫耐受的维持中发挥重要作用。近年来新的功能学研究发现,CD4^(+)Treg在一些疾病中并不能起到抑...传统观点认为,CD4^(+)调节性T细胞(regulatory T cell,Treg)是1类能够感知免疫应答强弱并具有负向调控作用的细胞亚群,在机体免疫自稳及免疫耐受的维持中发挥重要作用。近年来新的功能学研究发现,CD4^(+)Treg在一些疾病中并不能起到抑制免疫反应的作用,反而能够促进炎症损伤或抑制组织重塑,表明CD4^(+)Treg存在功能异质性。文章将回顾相关研究,对CD4^(+)Treg异质性、分群及与相关疾病的关系展开综述。展开更多
免疫耐受是器官移植临床研究的终极目标,是改善移植患者预后生存的关键。调节性T细胞(regulatory T cells,Tregs)在诱导免疫耐受过程中发挥着重要作用,如何诱导供体产生特异性Tregs是诱导免疫耐受突破的关键。尽管近20年来已经证实CD8^+...免疫耐受是器官移植临床研究的终极目标,是改善移植患者预后生存的关键。调节性T细胞(regulatory T cells,Tregs)在诱导免疫耐受过程中发挥着重要作用,如何诱导供体产生特异性Tregs是诱导免疫耐受突破的关键。尽管近20年来已经证实CD8^+Tregs在移植诱导免疫耐受中发挥重要的作用,但其特性仍然存在很大的争议。本文拟对CD8^+Tregs移植免疫耐受研究进展作一综述,探讨其在诱导肝移植免疫耐受中的潜力。展开更多
基金the colleagues from the Department of Pathology of Xijing Hospital of Fourth Military Medical University for their excellent technical support
文摘AIM: To study the biological and clinical characteristics of transcription factor forkhead box protein 3 (FOXP3) in hepatocellular carcinoma (HCC). METHODS: We analyzed the expression and localization of FOXP3 in HCC tissues and cell lines to evaluate its biological features. The relationship between FOXP3 staining and clinical risk factors of HCC was assessedto identify the clinical characteristics of FOXP3 in HCC. RESULTS: The mRNA and protein expression of FOXP3 were found in some hepatoma cell lines. Immunohistochemical (IHC) analysis of HCC sections revealed that 48% of HCC displayed FOXP3 staining, but we did not find any FOXP3 staining in normal liver tissues and para-tumor tissues. IHC and Confocal analysis showed that the expressions of FOXP3 were mainly present in the nucleus and cytoplasm of tumor cells in tissues or cell lines. In HCC, the distribution of FOXP3 was similar to that of the cirrhosis, but not to the hepatitis B virus. Those findings implicate that FOXP3 staining seems to be associated with the high risk of HCC. CONCLUSION: The clinical characteristics of FOXP3 in HCC warrants further studies to explore its functions and roles in the cirrhosis and development of HCC.
文摘Background Regulatory T cells （Tregs） are immunologically and clinically interesting not least because of the important role they play in allograft rejection. Likewise, expression of the transcription factor forkhead box protein 3 （FOXP3）, detected in transplant biopsies, is also of intere.（;t because of its role in the development of regulatory T cells. In this study, we investigated the relationship between FoxP3 mRNA expression and acute organ rejection in kidney-transplant recipients. Methods In this prospective study, FoxP3 mRNA expression levels in peripheral blood samples from 10 recipients of living relative-donor kidney transplants were measured before transplantation as well as at the 14th and 90th days post-transplantation. In addition, 46 first-time kidney-transplant recipients participated in a cross-sectional study, with 28 patients classified as having acute organ rejection; whilst the remaining 18 patients had functionally stable allografts. FoxP3 mRNA expression levels in peripheral blood samples were compared between these two different groups. Results Before transplantation mean FoxP3 mRNA levels vs. GADPH mRNA levels （Ig（FoxP3 mRNA/GADPH mRNA）） in the 10 recipients were 1.11±0.67. The mean FoxP3 mRNA expression levels measured at 14th and 90th days post-transplantation were significantly higher than before transplantation （1.69±0.38, P=0.03; 1.44±0.21, P=0.04, respectively）. Additionally, the mean FoxP3 mRNA levels vs. GADPH mRNA expression levels （Ig（FoxP3 mRNA/GADPH mRNA）） were significantly higher in recipients suffering acute rejection compared with those with stable allografts （1.77±0.61 and 1.43±0.27, respectively, P=0.03）. Conclusions After kidney transplantntion, FoxP3 mRNA levels were found to increase in the peripheral blood of all recipients. Considerably higher FoxP3 rnRNA levels were observed in recipients suffering acute rejection. These results suggest that FoxP3 mRNA levels in peripheral blood samples can be used as a diagnostic tool for i
文摘传统观点认为,CD4^(+)调节性T细胞(regulatory T cell,Treg)是1类能够感知免疫应答强弱并具有负向调控作用的细胞亚群,在机体免疫自稳及免疫耐受的维持中发挥重要作用。近年来新的功能学研究发现,CD4^(+)Treg在一些疾病中并不能起到抑制免疫反应的作用,反而能够促进炎症损伤或抑制组织重塑,表明CD4^(+)Treg存在功能异质性。文章将回顾相关研究,对CD4^(+)Treg异质性、分群及与相关疾病的关系展开综述。
文摘免疫耐受是器官移植临床研究的终极目标,是改善移植患者预后生存的关键。调节性T细胞(regulatory T cells,Tregs)在诱导免疫耐受过程中发挥着重要作用,如何诱导供体产生特异性Tregs是诱导免疫耐受突破的关键。尽管近20年来已经证实CD8^+Tregs在移植诱导免疫耐受中发挥重要的作用,但其特性仍然存在很大的争议。本文拟对CD8^+Tregs移植免疫耐受研究进展作一综述,探讨其在诱导肝移植免疫耐受中的潜力。
