摘要
目的评估视神经脊髓炎谱系疾病(NMOSD)患者中CD4^(+)CD25^(+)FoxP3^(+)调节性T细胞(Treg)的百分比,以及叉头蛋白3(FoxP3)mRNA的表达水平。方法采用病例对照研究法以20例NMOSD患者(NMOSD组)和20例健康受试者(正常对照组)为研究对象,收集外周血单个核细胞,使用荧光素记的CD4、CD25和FoxF3单克隆抗体,采用流式细胞术检测CD4^(+)CD25^(+)FoxP3^(+)Treg细胞百分比,采用实时聚合酶链反应法检测FoxP3基因表达。通过酶联免疫吸附试验检测转化生长因子β1(TGF-β1)、干扰素γ(IFN-γ)和白介素10(IL-10)的水平。结果与正常对照组相比,NMOSD组CD4^(+)CD25^(+)FoxP3^(+)Treg细胞百分比和FoxP3 mRNA表达水平显著降低,差异有统计学意义(P<0.05)。NMOSD组IFN-γ水平显著升高(P<0.05),IL-10水平降低(P<0.05),但TGF-β1水平无明显差异(P>0.05)。结论NMOSD可能与循环CD4^(+)CD25^(+)FoxP3^(+)Treg细胞功能的丧失以及FoxP3基因表达水平降低有关。
Objective To assess the percentage of CD4^(+)CD25^(+)FoxP3^(+)regulatory T(Treg)cells and the mRNA expression of forkhead box protein 3(FoxP3)in patients with neuromyelitis optica spectrum disorders(NMOSD).Methods The case-control study compared 20 NMOSD patients with 20 healthy controls.The mononuclear cells in peripheral blood were collected.The percentage of CD4^(+)CD25^(+)FoxP3^(+)Treg cells was measured with flow cytometry using specific monoclonal antibodies recognizing CD4,CD25 and FoxP3 markers.FoxP3 gene expression was determined with real-time polymerase chain reaction.The expressions of transforming growth factorβ1(TGF-β1),interferonγ(IFN-γ),and interleukin 10(IL-10)were determined with enzyme-linked immunosorbent assay.Results The percentage of CD4^(+)CD25^(+)FoxP3^(+)Treg cells and the mRNA expression of FoxP3 were significantly decreased in NMOSD patients compared with those in the controls(P<0.05).NMOSD patients had significantly increased IFN-γ(P<0.05)but decreased IL-10(P<0.05)levels,while there was no significant difference in the level of TGF-β1.Conclusion NMOSD may be associated with an abrogated function of circulating CD4^(+)CD25^(+)FoxP3^(+)Treg cells and a decreased level of FoxP3 gene expression.
作者
李鸿皓
于晶
陈亚丽
郭守刚
LI Honghao;YU Jing;CHEN Yali;GUO Shougang(Department of Neurology,Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021,Shandong,China;Department of Pathology and Pathophysiology,Shandong Medical College,Jinan,250002,Shandong,China)
出处
《山东大学学报（医学版）》
CAS
北大核心
2022年第4期50-54,共5页
Journal of Shandong University:Health Sciences
基金
山东医药卫生科技发展计划项目(2017WS028)
