A system for transformation and regeneration of Lycium barbarum L. , an important Chinese medical plant, has been established. Young stem segments from Lycium barbarum L. were infected with Agrobacterium tumefaciens C...A system for transformation and regeneration of Lycium barbarum L. , an important Chinese medical plant, has been established. Young stem segments from Lycium barbarum L. were infected with Agrobacterium tumefaciens C58cl(pGV3850: :ineo1130), and the transformed calli selected from the callus induction medium containing 50 fig/ml kanamycin could regenerate buds on differentiation medium containing 25 μg/ml kanamycin. 30% of the regenerated buds were normal in morphology. The normal buds could develop into whole plantlets after they were transferred to the rooting medium to induce roots. Nopa-line detection, NPT-Ⅱ enzyme activity assay and Southern blotting hybridization indicated that the foreign genes had been integrated into the genome of Lycium barbarum L. and expressed in the plant. In the processes of experiments, it was found that (i) after the pre-processes, the explants which formed callus quickly were easy to transform ; (Ⅱ) the rate of normal regenerated plants from transgenic calli was higher than that from the untransgenic ones.展开更多
In recent years, research that focused on applying 'disarmed' and constructed Tiplasmid of Agrobacterium tumefaciens as a dominant vector for plant genetic engineering has developed quickly. Several vector sys...In recent years, research that focused on applying 'disarmed' and constructed Tiplasmid of Agrobacterium tumefaciens as a dominant vector for plant genetic engineering has developed quickly. Several vector systems have become especially useful in plant transformation, and a great number of foreign genes have been transferred to dicot plants by means of the leaf-disc transformation method along with the Ti-plasmid vector. Most of the transgenic plants, which bear the novel trait, can accomplish complete regeneration, and the characteristics of the new genotype can be transmitted from generation to generation in the plant cells.展开更多
文摘A system for transformation and regeneration of Lycium barbarum L. , an important Chinese medical plant, has been established. Young stem segments from Lycium barbarum L. were infected with Agrobacterium tumefaciens C58cl(pGV3850: :ineo1130), and the transformed calli selected from the callus induction medium containing 50 fig/ml kanamycin could regenerate buds on differentiation medium containing 25 μg/ml kanamycin. 30% of the regenerated buds were normal in morphology. The normal buds could develop into whole plantlets after they were transferred to the rooting medium to induce roots. Nopa-line detection, NPT-Ⅱ enzyme activity assay and Southern blotting hybridization indicated that the foreign genes had been integrated into the genome of Lycium barbarum L. and expressed in the plant. In the processes of experiments, it was found that (i) after the pre-processes, the explants which formed callus quickly were easy to transform ; (Ⅱ) the rate of normal regenerated plants from transgenic calli was higher than that from the untransgenic ones.
文摘In recent years, research that focused on applying 'disarmed' and constructed Tiplasmid of Agrobacterium tumefaciens as a dominant vector for plant genetic engineering has developed quickly. Several vector systems have become especially useful in plant transformation, and a great number of foreign genes have been transferred to dicot plants by means of the leaf-disc transformation method along with the Ti-plasmid vector. Most of the transgenic plants, which bear the novel trait, can accomplish complete regeneration, and the characteristics of the new genotype can be transmitted from generation to generation in the plant cells.
