AIM： To determine whether the carbon monoxide （CO）-releasing molecules （CORM）-Iiberated CO sup- press inflammatory responses in the small intestine of septic mice. METHODS： The C57BL/6 mice （male, n = 36; weigh...AIM： To determine whether the carbon monoxide （CO）-releasing molecules （CORM）-Iiberated CO sup- press inflammatory responses in the small intestine of septic mice. METHODS： The C57BL/6 mice （male, n = 36; weight 20±2 g） were assigned to four groups in three re- spective experiments. Sepsis in mice was induced by cecal ligation and puncture （CLP） （24 h）. Tricarbonyl- dichlororuthenium （Ⅱ） dimer （CORM-2） （8 mg/kg, i. v.） was administrated immediately after induction of CLP. The levels of inflammatory cytokines [interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α）] in tis- sue homogenates were measured with enzyme-linked immunosorbent assay. The levels of malondialdehyde （MDA） in the tissues were determined. The levels of nitric oxide （NO） in tissue homogenate were measured and the expression levels of intercellular adhesion mol- ecule 1 （ICAM-1） and inducible nitric oxide synthase （iNOS） in the small intestine were also assessed. NO and IL-8 levels in the supernatants were determined after the human adenocarcinoma cell line Caco-2 was stimulated by lipopolysaccharide （LPS） （10 g/mL） for 4 h in vitro. RESULTS： At 24 h after CLP, histological analysis showed that the ileum and jejunum from CLP mice in- duced severe edema and sloughing of the villous tips, as well as infiltration of inflammatory cells into the mu- cosa. Semi-quantitative analysis of histological samples of ileum and jejunum showed that granulocyte infil- tration in the septic mice was significantly increased compared to that in the sham group. Administration of CORM-2 significantly decreased granulocyte infiltration. At 24 h after CLP, the tissue MDA levels in the mid- ileum and mid-jejunum significantly increased com- pared to the sham animals （103.68 ± 23.88 nmol/ml vs 39.66 ± 8.23 nmol/mL, 89.66±9.98 nmol/mL vs 32.32 ± 7.43 nmol/mL, P 〈 0.01）. In vitro administra- tion of CORM-2, tissue MDA levels were significantly decreased （50.65±11.46 nmol/mL, 59.32 展开更多
To grade Small Hepatocellular Car Cinoma(SHCC)using texture analysis of CT images,we retrospectively analysed 68 cases of Grade II(medium-differentiation)and 37 cases of Grades III and IV(high-differentiation).The gra...To grade Small Hepatocellular Car Cinoma(SHCC)using texture analysis of CT images,we retrospectively analysed 68 cases of Grade II(medium-differentiation)and 37 cases of Grades III and IV(high-differentiation).The grading scheme follows 4 stages:(1)training a Super Resolution Generative Adversarial Network(SRGAN)migration learning model on the Lung Nodule Analysis 2016 Dataset,and employing this model to reconstruct Super Resolution Images of the SHCC Dataset(SR-SHCC)images;(2)designing a texture clustering method based on Gray-Level Co-occurrence Matrix(GLCM)to segment tumour regions,which are Regions Of Interest(ROIs),from the original and SR-SHCC images,respectively;(3)extracting texture features on the ROIs;(4)performing statistical analysis and classifications.The segmentation achieved accuracies of 0.9049 and 0.8590 in the original SHCC images and the SR-SHCC images,respectively.The classification achived an accuracy of 0.838 and an Area Under the ROC Curve(AUC)of 0.84.The grading scheme can effectively reduce poor impacts on the texture analysis of SHCC ROIs.It may play a guiding role for physicians in early diagnoses of medium-differentiation and high-differentiation in SHCC.展开更多
基金Supported by National Natural Science Foundation of China, No.30772256,No.81071546 and No.81272148
文摘AIM： To determine whether the carbon monoxide （CO）-releasing molecules （CORM）-Iiberated CO sup- press inflammatory responses in the small intestine of septic mice. METHODS： The C57BL/6 mice （male, n = 36; weight 20±2 g） were assigned to four groups in three re- spective experiments. Sepsis in mice was induced by cecal ligation and puncture （CLP） （24 h）. Tricarbonyl- dichlororuthenium （Ⅱ） dimer （CORM-2） （8 mg/kg, i. v.） was administrated immediately after induction of CLP. The levels of inflammatory cytokines [interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α）] in tis- sue homogenates were measured with enzyme-linked immunosorbent assay. The levels of malondialdehyde （MDA） in the tissues were determined. The levels of nitric oxide （NO） in tissue homogenate were measured and the expression levels of intercellular adhesion mol- ecule 1 （ICAM-1） and inducible nitric oxide synthase （iNOS） in the small intestine were also assessed. NO and IL-8 levels in the supernatants were determined after the human adenocarcinoma cell line Caco-2 was stimulated by lipopolysaccharide （LPS） （10 g/mL） for 4 h in vitro. RESULTS： At 24 h after CLP, histological analysis showed that the ileum and jejunum from CLP mice in- duced severe edema and sloughing of the villous tips, as well as infiltration of inflammatory cells into the mu- cosa. Semi-quantitative analysis of histological samples of ileum and jejunum showed that granulocyte infil- tration in the septic mice was significantly increased compared to that in the sham group. Administration of CORM-2 significantly decreased granulocyte infiltration. At 24 h after CLP, the tissue MDA levels in the mid- ileum and mid-jejunum significantly increased com- pared to the sham animals （103.68 ± 23.88 nmol/ml vs 39.66 ± 8.23 nmol/mL, 89.66±9.98 nmol/mL vs 32.32 ± 7.43 nmol/mL, P 〈 0.01）. In vitro administra- tion of CORM-2, tissue MDA levels were significantly decreased （50.65±11.46 nmol/mL, 59.32
基金supported by the National Key R&D Program of China(No.2018YFC0807500)。
文摘To grade Small Hepatocellular Car Cinoma(SHCC)using texture analysis of CT images,we retrospectively analysed 68 cases of Grade II(medium-differentiation)and 37 cases of Grades III and IV(high-differentiation).The grading scheme follows 4 stages:(1)training a Super Resolution Generative Adversarial Network(SRGAN)migration learning model on the Lung Nodule Analysis 2016 Dataset,and employing this model to reconstruct Super Resolution Images of the SHCC Dataset(SR-SHCC)images;(2)designing a texture clustering method based on Gray-Level Co-occurrence Matrix(GLCM)to segment tumour regions,which are Regions Of Interest(ROIs),from the original and SR-SHCC images,respectively;(3)extracting texture features on the ROIs;(4)performing statistical analysis and classifications.The segmentation achieved accuracies of 0.9049 and 0.8590 in the original SHCC images and the SR-SHCC images,respectively.The classification achived an accuracy of 0.838 and an Area Under the ROC Curve(AUC)of 0.84.The grading scheme can effectively reduce poor impacts on the texture analysis of SHCC ROIs.It may play a guiding role for physicians in early diagnoses of medium-differentiation and high-differentiation in SHCC.
