核桃腐烂病的发生严重影响核桃树的生长与结果。将染病树皮进行培养,划线分离出一株真菌,应用PCR技术对分离菌株的线粒体基因[the small r RNA subunit(rns)]克隆后测序,并将所测序列与美国国家生物技术信息中心(Natinal Center for Bio...核桃腐烂病的发生严重影响核桃树的生长与结果。将染病树皮进行培养,划线分离出一株真菌,应用PCR技术对分离菌株的线粒体基因[the small r RNA subunit(rns)]克隆后测序,并将所测序列与美国国家生物技术信息中心(Natinal Center for Biotechnology Information,NCBI)数据库中已有的序列进行分析比较,利用Neighbor-joining建立系统发育树。结果表明,分离出的菌株与茎点霉属(Phoma sp.)聚集在同一支上,可以确定是茎点霉属(Phoma sp.)。展开更多
Objective:To evaluate the antiniicrobial polenlial of different extracts of the endophytic fungus Phoma sp.and the tentative identification of their active constituents.Methods:The extract and compounds were screened ...Objective:To evaluate the antiniicrobial polenlial of different extracts of the endophytic fungus Phoma sp.and the tentative identification of their active constituents.Methods:The extract and compounds were screened for antimicrobial activity using the Agar Well Diffusion Method.Four compounds were purified using column chromatography and tlieir structures were assigned using～1H and～(13)C NMR spectra,DEPT,2D COSY,HMQC and HMBC experiments.Results:The ethyl acetate fraction of Phoma sp.showed good antifungal,antibacterial,and algicidal properties.One new dihydrofuran derivative,named phomafuranol(1),together with tliree known compounds,phomalacton(2),(3R)-5-hydroxymellein(3)and emodin(4)were isolated from the ethyl acetate fraction of Phoma sp.Preliminary studies indicated that phomalacton(2)displayed strong antibacterial,good antifungal and antialgal activities.Similarly(3R)-5-hydroxymellein(3)and emodin(4)showed good antifungal,antibacterial and algicidal properties.Conclusions:Antimicrobial activities of the ethyl acetate fraction of the endophytic fungus Phoma sp.and isolated compounds clearly demonstrate that Phoma sp.and its active compounds represent a great potential for the food,cosmetic and pharmaceutical industries.展开更多
文摘核桃腐烂病的发生严重影响核桃树的生长与结果。将染病树皮进行培养,划线分离出一株真菌,应用PCR技术对分离菌株的线粒体基因[the small r RNA subunit(rns)]克隆后测序,并将所测序列与美国国家生物技术信息中心(Natinal Center for Biotechnology Information,NCBI)数据库中已有的序列进行分析比较,利用Neighbor-joining建立系统发育树。结果表明,分离出的菌株与茎点霉属(Phoma sp.)聚集在同一支上,可以确定是茎点霉属(Phoma sp.)。
基金supported by the BMBF(Bundesministerium fr Bildung und Forschung,project no.03F0360A)
文摘Objective:To evaluate the antiniicrobial polenlial of different extracts of the endophytic fungus Phoma sp.and the tentative identification of their active constituents.Methods:The extract and compounds were screened for antimicrobial activity using the Agar Well Diffusion Method.Four compounds were purified using column chromatography and tlieir structures were assigned using～1H and～(13)C NMR spectra,DEPT,2D COSY,HMQC and HMBC experiments.Results:The ethyl acetate fraction of Phoma sp.showed good antifungal,antibacterial,and algicidal properties.One new dihydrofuran derivative,named phomafuranol(1),together with tliree known compounds,phomalacton(2),(3R)-5-hydroxymellein(3)and emodin(4)were isolated from the ethyl acetate fraction of Phoma sp.Preliminary studies indicated that phomalacton(2)displayed strong antibacterial,good antifungal and antialgal activities.Similarly(3R)-5-hydroxymellein(3)and emodin(4)showed good antifungal,antibacterial and algicidal properties.Conclusions:Antimicrobial activities of the ethyl acetate fraction of the endophytic fungus Phoma sp.and isolated compounds clearly demonstrate that Phoma sp.and its active compounds represent a great potential for the food,cosmetic and pharmaceutical industries.
