Phytosulfokine- α （PSK- α ）, a biologically active peptide acting as a growth factor, plays a key role in cellular differentiation and proliferation. To test if PSK- α has some influence on agrobacterium-mediated...Phytosulfokine- α （PSK- α ）, a biologically active peptide acting as a growth factor, plays a key role in cellular differentiation and proliferation. To test if PSK- α has some influence on agrobacterium-mediated transformation in rice, PSK-α at a series of concentrations was added into co-culture medium respectively. The results showed that PSK- α indeed affected the recovery of resistant calli and the transformation frequency of rice varieties Taipei 309 and Lijiangxintuanheigu, PSK- α at the concentration of 10 nmol/L could increase induction of resistant callus and efficiency of transformation, with a 11% and 4.9% top increase, respectively than the control. However, PSK- αat 200 nmol/L could inhibit the induction of the resistant calli. Further more, the effect of PSK-α on agrobacterium-mediated transformation is related with the concentration of 2, 4-D in selection medium. Higher induction rate of resistant calli was obtained from tissues treated with PSK- α plus 2 mg/L 2, 4-D.展开更多
Phytosulfokine-α(PSK-α),a sulfated pentapeptide with the sequence YIYTQ,is encoded by a small precursor gene family in Arabidopsis.PSK-αregulates multiple growth and developmental processes as a novel peptide hormo...Phytosulfokine-α(PSK-α),a sulfated pentapeptide with the sequence YIYTQ,is encoded by a small precursor gene family in Arabidopsis.PSK-αregulates multiple growth and developmental processes as a novel peptide hormone.Despite its importance,functions of PSK-αin M.truncatula growth remains unknown.In this study,we identified five genes to encode PSK-αprecursors in M.truncatula.All of these precursors possess conserved PSK-αsignature motif.Expression pattern analysis of these MtPSK genes revealed that each gene was expressed in a tissue-specific or ubiquitous pattern and three of them were remarkably expressed in root.Treatment of M.truncatula seedlings with synthetic PSK-αpeptide significantly promoted root elongation.In addition,expression analysis of downstream genes by RNA-seq and qRT-PCR assays suggested that PSK-αsignaling might regulate cell wall structure via PMEI-PME module to promote root cell growth.Taken together,our results shed light on the mechanism by which PSK-αpromotes root growth in M.truncatula,providing a new resource for improvement of root growth in agriculture.展开更多
植物磺肽素(phytosulfokine,PSK)是一种新型植物肽类生长调节因子,成熟的PSK是由5个或4个氨基酸构成的磺化短肽[Tyr(SO_(3)H)-Ile-Tyr(SO_(3)H)-Thr-Gln],其中的酪氨酸(Tyr)被磺化修饰。PSK在高等植物中广泛分布,但在低等植物中还未见...植物磺肽素(phytosulfokine,PSK)是一种新型植物肽类生长调节因子,成熟的PSK是由5个或4个氨基酸构成的磺化短肽[Tyr(SO_(3)H)-Ile-Tyr(SO_(3)H)-Thr-Gln],其中的酪氨酸(Tyr)被磺化修饰。PSK在高等植物中广泛分布,但在低等植物中还未见报道。其在植物中通过细胞膜上的富含亮氨酸的类受体激酶(leucine-rich receptor like kinase protein,LRR-RLK)蛋白家族作为受体向胞内传递信号。拟南芥中报道的PSK受体PSKR具有激酶活性和鸟苷酸环化酶活性双重信号感知作用。PSK调控植物多种生理和生化反应,包括植物细胞的体外分化、细胞的生长、根顶端分生组织细胞和静止中心细胞的调控、花粉管细胞的发育、植物体免疫的调控等;参与非生物胁迫应答,以及和生长素、细胞分裂素、茉莉酸、油菜素内脂等植物激素的协同调控等等。本文总结了近年来PSK在植物生长、发育和逆境应答等中的功能与作用机制的研究进展,并对未来的研究方向作了展望,希望能为今后PSK相关研究提供指导。展开更多
文摘Phytosulfokine- α （PSK- α ）, a biologically active peptide acting as a growth factor, plays a key role in cellular differentiation and proliferation. To test if PSK- α has some influence on agrobacterium-mediated transformation in rice, PSK-α at a series of concentrations was added into co-culture medium respectively. The results showed that PSK- α indeed affected the recovery of resistant calli and the transformation frequency of rice varieties Taipei 309 and Lijiangxintuanheigu, PSK- α at the concentration of 10 nmol/L could increase induction of resistant callus and efficiency of transformation, with a 11% and 4.9% top increase, respectively than the control. However, PSK- αat 200 nmol/L could inhibit the induction of the resistant calli. Further more, the effect of PSK-α on agrobacterium-mediated transformation is related with the concentration of 2, 4-D in selection medium. Higher induction rate of resistant calli was obtained from tissues treated with PSK- α plus 2 mg/L 2, 4-D.
基金This work is supported by the National Natural Science Foundation of China(31500197).
文摘Phytosulfokine-α(PSK-α),a sulfated pentapeptide with the sequence YIYTQ,is encoded by a small precursor gene family in Arabidopsis.PSK-αregulates multiple growth and developmental processes as a novel peptide hormone.Despite its importance,functions of PSK-αin M.truncatula growth remains unknown.In this study,we identified five genes to encode PSK-αprecursors in M.truncatula.All of these precursors possess conserved PSK-αsignature motif.Expression pattern analysis of these MtPSK genes revealed that each gene was expressed in a tissue-specific or ubiquitous pattern and three of them were remarkably expressed in root.Treatment of M.truncatula seedlings with synthetic PSK-αpeptide significantly promoted root elongation.In addition,expression analysis of downstream genes by RNA-seq and qRT-PCR assays suggested that PSK-αsignaling might regulate cell wall structure via PMEI-PME module to promote root cell growth.Taken together,our results shed light on the mechanism by which PSK-αpromotes root growth in M.truncatula,providing a new resource for improvement of root growth in agriculture.
文摘植物磺肽素(phytosulfokine,PSK)是一种新型植物肽类生长调节因子,成熟的PSK是由5个或4个氨基酸构成的磺化短肽[Tyr(SO_(3)H)-Ile-Tyr(SO_(3)H)-Thr-Gln],其中的酪氨酸(Tyr)被磺化修饰。PSK在高等植物中广泛分布,但在低等植物中还未见报道。其在植物中通过细胞膜上的富含亮氨酸的类受体激酶(leucine-rich receptor like kinase protein,LRR-RLK)蛋白家族作为受体向胞内传递信号。拟南芥中报道的PSK受体PSKR具有激酶活性和鸟苷酸环化酶活性双重信号感知作用。PSK调控植物多种生理和生化反应,包括植物细胞的体外分化、细胞的生长、根顶端分生组织细胞和静止中心细胞的调控、花粉管细胞的发育、植物体免疫的调控等;参与非生物胁迫应答,以及和生长素、细胞分裂素、茉莉酸、油菜素内脂等植物激素的协同调控等等。本文总结了近年来PSK在植物生长、发育和逆境应答等中的功能与作用机制的研究进展,并对未来的研究方向作了展望,希望能为今后PSK相关研究提供指导。
