OBJECTIVE： To observe the impact of Shijueming （Concha Haliotidis） on spontaneously hypertensive rats via blood pressure, serum calcium, vascular smooth muscle membrane L-type calcium channel α1 C subunit （CaL-...OBJECTIVE： To observe the impact of Shijueming （Concha Haliotidis） on spontaneously hypertensive rats via blood pressure, serum calcium, vascular smooth muscle membrane L-type calcium channel α1 C subunit （CaL-α1C）, plasma membrane calci- um-ATPase （PMCA） mRNA expression, and the L-type calcium channel in vascular smooth muscle cells. METHODS： Twelve-week-old male rats with sponta- neous hypertension were divided into three groups： a Shijueming （Concha Haliotidis） group （group 1）, a nifedipine group （group 2）, and a dis- tilled water group （group 3）. All were given a four-week treatment. Blood pressure and dissocia- tive serum calcium were examined before treat- ment. Blood pressure was taken every week during treatment. Atomic absorption spectrometry was used to examine dissociative serum calcium. Re-verse transcription-polymerase chain reaction was used to examine the expression of CaL-α1C and PM- CA1 mRNA. The patch clamp technique was used to examine the electrophysiological characteristics of the vascular smooth muscle cell calcium chan- nels. RESULTS： After treatment, blood pressure of the Shijueming （Concha Halioticlis） group lowered but not significantly （P〉0.05）. Blood pressure of the nifedipine group lowered significantly （P〈0.05）. Blood pressure of the distilled water group re- mained high. The concentration of serum calcium in the Shijueming （Concha Haliotidis） and the dis- tilled water groups lowered （P〈0.05）. Expression of CaL-α1C mRNA in the nifedipine group decreased compared with the distilled water group （P〈0.01）. There was the decreasing trend in the Shijueming （Concha Haliotidis） group, but it was not statistically significant. Shijueming （Concha Haliotidis） also had effects on the expression of PMCA1 mRNA but with- out statistical significance. However, there was a significant decreasing effect on vascular smooth muscle cell Ica-L flow. CONCLUSION： This study indicated that Shijuem- ing （Concha Haliotidis） c展开更多
Objective: Haliotidis, the dried muscle of abalone, has long been used as a marine traditional Chinese medicine(TCM) due to its high medicinal and nutritive values, whereas few reports contributed to its quality contr...Objective: Haliotidis, the dried muscle of abalone, has long been used as a marine traditional Chinese medicine(TCM) due to its high medicinal and nutritive values, whereas few reports contributed to its quality control. This study aimed to establish the high-performance liquid chromatography(HPLC) fingerprints of Haliotidis. Materials and Methods: The HPLC fingerprints of Haliotidis, the manufacturing products of dried muscle of abalone, were analyzed by reverse-phase HPLC, using a YMC HPLC C18 column(250 mm × 4.6 mm, 5 μm).The gradient elution mode was applied with mobile phase starting from 5% acetonitrile and 0.1% phosphoric acid aqueous solution to 100%acetonitrile in 70 min at a flow rate of 1.0 mL/min. The detection wavelength was set at 190 nm. Results: Through compared the HPLC fingerprint chromatograms of 14 batches of Haliotidis, 25 peaks were assigned as the characteristic common peaks, of which one index component L-phenylalanine was identified by comparison with its standard substance. The established HPLC fingerprint analysis method of Haliotidis was validated with good precision, repeatability, and stability. Based on the above study, a reference HPLC fingerprint chromatogram of Haliotidis was proposed. Conclusions: It is the first time to establish the HPLC fingerprint chromatogram of TCM Haliotidis, which could contribute to the establishment of its quality control system.展开更多
目的利用拉曼光谱建立多种算法的定性模型,用于贝壳类动物药牡蛎和石决明的鉴别分析。方法采集32批牡蛎和29批石决明共计366个样品的拉曼光谱,光谱在Matlab软件中作Savitzky-Golay平滑滤波、Scale Normalization for ImagePyramids基线...目的利用拉曼光谱建立多种算法的定性模型,用于贝壳类动物药牡蛎和石决明的鉴别分析。方法采集32批牡蛎和29批石决明共计366个样品的拉曼光谱,光谱在Matlab软件中作Savitzky-Golay平滑滤波、Scale Normalization for ImagePyramids基线校正、归一化预处理,并对全部及特征波段进行主成分分析降维。使用K最邻近法、决策树算法、判别分析法、集成学习法、支持向量机、人工神经网络6种分类算法建立鉴别模型,通过贝叶斯优化对前5种模型性能进行提升。结果预处理后拉曼信号峰明显,降维后牡蛎和石决明有聚类趋势。建立以200~310,670~740,1050~1100 cm^(-1)为特征波段的5种算法鉴别模型,相比于全波段模型,除集成学习法、支持向量机外,其他算法准确率均有一定的提高。贝叶斯优化后,有3种模型训练集准确率达到98%,测试集准确率达到100%。采用人工神经网络建模分类,训练集、验证集、测试集的正确率均达到100%,能够准确地区分牡蛎和石决明。结论基于多种算法或IP建立了牡蛎及石决明拉曼光谱鉴别模型,预测效果均较为理想,其中人工神经网络模型可实现牡蛎和石决明的百分百准确、快速鉴别。展开更多
文摘OBJECTIVE： To observe the impact of Shijueming （Concha Haliotidis） on spontaneously hypertensive rats via blood pressure, serum calcium, vascular smooth muscle membrane L-type calcium channel α1 C subunit （CaL-α1C）, plasma membrane calci- um-ATPase （PMCA） mRNA expression, and the L-type calcium channel in vascular smooth muscle cells. METHODS： Twelve-week-old male rats with sponta- neous hypertension were divided into three groups： a Shijueming （Concha Haliotidis） group （group 1）, a nifedipine group （group 2）, and a dis- tilled water group （group 3）. All were given a four-week treatment. Blood pressure and dissocia- tive serum calcium were examined before treat- ment. Blood pressure was taken every week during treatment. Atomic absorption spectrometry was used to examine dissociative serum calcium. Re-verse transcription-polymerase chain reaction was used to examine the expression of CaL-α1C and PM- CA1 mRNA. The patch clamp technique was used to examine the electrophysiological characteristics of the vascular smooth muscle cell calcium chan- nels. RESULTS： After treatment, blood pressure of the Shijueming （Concha Halioticlis） group lowered but not significantly （P〉0.05）. Blood pressure of the nifedipine group lowered significantly （P〈0.05）. Blood pressure of the distilled water group re- mained high. The concentration of serum calcium in the Shijueming （Concha Haliotidis） and the dis- tilled water groups lowered （P〈0.05）. Expression of CaL-α1C mRNA in the nifedipine group decreased compared with the distilled water group （P〈0.01）. There was the decreasing trend in the Shijueming （Concha Haliotidis） group, but it was not statistically significant. Shijueming （Concha Haliotidis） also had effects on the expression of PMCA1 mRNA but with- out statistical significance. However, there was a significant decreasing effect on vascular smooth muscle cell Ica-L flow. CONCLUSION： This study indicated that Shijuem- ing （Concha Haliotidis） c
基金financially supported by the National Natural Science Fund of China (No. 41806191)the National High Technology Research and Development Program of China (863 Program)(No. 2013AA093001)+2 种基金the Taishan Scholars Program,Chinathe Program of Open Studio for Druggability Research of Marine Natural ProductsPilot National Laboratory for Marine Science and Technology (Qingdao, China) Directed by Kai-Xian Chen and Yue-Wei Guo。
文摘Objective: Haliotidis, the dried muscle of abalone, has long been used as a marine traditional Chinese medicine(TCM) due to its high medicinal and nutritive values, whereas few reports contributed to its quality control. This study aimed to establish the high-performance liquid chromatography(HPLC) fingerprints of Haliotidis. Materials and Methods: The HPLC fingerprints of Haliotidis, the manufacturing products of dried muscle of abalone, were analyzed by reverse-phase HPLC, using a YMC HPLC C18 column(250 mm × 4.6 mm, 5 μm).The gradient elution mode was applied with mobile phase starting from 5% acetonitrile and 0.1% phosphoric acid aqueous solution to 100%acetonitrile in 70 min at a flow rate of 1.0 mL/min. The detection wavelength was set at 190 nm. Results: Through compared the HPLC fingerprint chromatograms of 14 batches of Haliotidis, 25 peaks were assigned as the characteristic common peaks, of which one index component L-phenylalanine was identified by comparison with its standard substance. The established HPLC fingerprint analysis method of Haliotidis was validated with good precision, repeatability, and stability. Based on the above study, a reference HPLC fingerprint chromatogram of Haliotidis was proposed. Conclusions: It is the first time to establish the HPLC fingerprint chromatogram of TCM Haliotidis, which could contribute to the establishment of its quality control system.
文摘目的利用拉曼光谱建立多种算法的定性模型,用于贝壳类动物药牡蛎和石决明的鉴别分析。方法采集32批牡蛎和29批石决明共计366个样品的拉曼光谱,光谱在Matlab软件中作Savitzky-Golay平滑滤波、Scale Normalization for ImagePyramids基线校正、归一化预处理,并对全部及特征波段进行主成分分析降维。使用K最邻近法、决策树算法、判别分析法、集成学习法、支持向量机、人工神经网络6种分类算法建立鉴别模型,通过贝叶斯优化对前5种模型性能进行提升。结果预处理后拉曼信号峰明显,降维后牡蛎和石决明有聚类趋势。建立以200~310,670~740,1050~1100 cm^(-1)为特征波段的5种算法鉴别模型,相比于全波段模型,除集成学习法、支持向量机外,其他算法准确率均有一定的提高。贝叶斯优化后,有3种模型训练集准确率达到98%,测试集准确率达到100%。采用人工神经网络建模分类,训练集、验证集、测试集的正确率均达到100%,能够准确地区分牡蛎和石决明。结论基于多种算法或IP建立了牡蛎及石决明拉曼光谱鉴别模型,预测效果均较为理想,其中人工神经网络模型可实现牡蛎和石决明的百分百准确、快速鉴别。
