Previous studies have demonstrated the protective effect of hypoxic preconditioning on acute cerebral infarction, but the mechanisms underlying this protection remain unclear. To investigate the protective mechanisms ...Previous studies have demonstrated the protective effect of hypoxic preconditioning on acute cerebral infarction, but the mechanisms underlying this protection remain unclear. To investigate the protective mechanisms of hypoxic preconditioning in relation to its effects on angiogenesis, we in- duced a photochemical model of cerebral infarction in an inbred line of mice （BALB/c）. Mice were then exposed to hypoxic preconditioning 30 minutes prior to model establishment. Results showed significantly increased vascular endothelial growth factor and CD31 expression in the ischemic penumbra at 24 and 72 hours post infarction, mainly in neurons and vascular endothelial cells. Hypoxic preconditioning increased vascular endothelial growth factor and CD31 expression in the ischemic penumbra and the expression of vascular endothelial growth factor was positively related to that of CD31. Moreover, hypoxic preconditioning reduced the infarct volume and improved neu- rological function in mice. These findings indicate that the protective role of hypoxic preconditioning in acute cerebral infarction may possibly be due to an increase in expression of vascular endothelial growth factor and CD31 in the ischemic penumbra, which promoted angiogenesis.展开更多
目的:探究消渴方加减对气阴两虚夹瘀型糖尿病肾病(DN)的内皮损伤、氧化应激及生化指标的影响。方法:随机将2017年9月至2018年6月河南省中医院收治的94例DN患者分为两组,各47例,对照组采用静脉滴注还原型谷胱甘肽注射液进行治疗,在对照...目的:探究消渴方加减对气阴两虚夹瘀型糖尿病肾病(DN)的内皮损伤、氧化应激及生化指标的影响。方法:随机将2017年9月至2018年6月河南省中医院收治的94例DN患者分为两组,各47例,对照组采用静脉滴注还原型谷胱甘肽注射液进行治疗,在对照组的基础上,观察组给予消渴方加减进行治疗。于治疗前后,观察两组的症状评分、空腹血糖(FBG),餐后2 h血糖(2 h PG),谷胱甘肽过氧化物酶(GSH-Px),超氧化物歧化酶(SOD),丙二醛(MDA),白细胞介素-1β(IL-1β),IL-6,超敏C反应蛋白(hs-CRP),肿瘤坏死因子-α(TNF-α),组织型血纤维蛋白溶酶原激活物(t-PA),1型组织纤溶酶原激活物抑制物(PAI-1),血肌酐(SCr),血尿素氮(BUN),一氧化氮(NO),内皮素-1(ET-1),24 h尿总蛋白水平,并观察两组的疗效。结果:治疗后,观察组治疗总有效率为89.36%,高于对照组的74.47%(Z=3.949,P<0.05);观察组治疗后的口渴喜饮、倦怠乏力、气短、心悸、心烦、失眠、头晕耳鸣等中医症状评分低于对照组(P<0.05);观察组治疗后的FBG,2 h PG,SCr,BUN,24 h尿总蛋白,ET-1,MDA,IL-1β,IL-6,hs-CRP,TNF-α,PAI-1水平低于对照组(P<0.05),NO,SOD,GSP-Px,t-PA水平升高(P<0.05)。结论:消渴方加减治疗气阴两虚夹瘀型DN效果显著,促进临床症状的改善,促进机体凝血纤溶系统平衡的恢复,抑制炎症反应及氧化应激反应,保护内皮功能及肾功能。展开更多
基金supported by the National Natural Science Foundation of China,No.30870854the Natural Science Foundation of Beijing,No.7111003the Natural Science Foundation of Shandong Province,No.ZR2010HM029
文摘Previous studies have demonstrated the protective effect of hypoxic preconditioning on acute cerebral infarction, but the mechanisms underlying this protection remain unclear. To investigate the protective mechanisms of hypoxic preconditioning in relation to its effects on angiogenesis, we in- duced a photochemical model of cerebral infarction in an inbred line of mice （BALB/c）. Mice were then exposed to hypoxic preconditioning 30 minutes prior to model establishment. Results showed significantly increased vascular endothelial growth factor and CD31 expression in the ischemic penumbra at 24 and 72 hours post infarction, mainly in neurons and vascular endothelial cells. Hypoxic preconditioning increased vascular endothelial growth factor and CD31 expression in the ischemic penumbra and the expression of vascular endothelial growth factor was positively related to that of CD31. Moreover, hypoxic preconditioning reduced the infarct volume and improved neu- rological function in mice. These findings indicate that the protective role of hypoxic preconditioning in acute cerebral infarction may possibly be due to an increase in expression of vascular endothelial growth factor and CD31 in the ischemic penumbra, which promoted angiogenesis.
文摘目的:探究消渴方加减对气阴两虚夹瘀型糖尿病肾病(DN)的内皮损伤、氧化应激及生化指标的影响。方法:随机将2017年9月至2018年6月河南省中医院收治的94例DN患者分为两组,各47例,对照组采用静脉滴注还原型谷胱甘肽注射液进行治疗,在对照组的基础上,观察组给予消渴方加减进行治疗。于治疗前后,观察两组的症状评分、空腹血糖(FBG),餐后2 h血糖(2 h PG),谷胱甘肽过氧化物酶(GSH-Px),超氧化物歧化酶(SOD),丙二醛(MDA),白细胞介素-1β(IL-1β),IL-6,超敏C反应蛋白(hs-CRP),肿瘤坏死因子-α(TNF-α),组织型血纤维蛋白溶酶原激活物(t-PA),1型组织纤溶酶原激活物抑制物(PAI-1),血肌酐(SCr),血尿素氮(BUN),一氧化氮(NO),内皮素-1(ET-1),24 h尿总蛋白水平,并观察两组的疗效。结果:治疗后,观察组治疗总有效率为89.36%,高于对照组的74.47%(Z=3.949,P<0.05);观察组治疗后的口渴喜饮、倦怠乏力、气短、心悸、心烦、失眠、头晕耳鸣等中医症状评分低于对照组(P<0.05);观察组治疗后的FBG,2 h PG,SCr,BUN,24 h尿总蛋白,ET-1,MDA,IL-1β,IL-6,hs-CRP,TNF-α,PAI-1水平低于对照组(P<0.05),NO,SOD,GSP-Px,t-PA水平升高(P<0.05)。结论:消渴方加减治疗气阴两虚夹瘀型DN效果显著,促进临床症状的改善,促进机体凝血纤溶系统平衡的恢复,抑制炎症反应及氧化应激反应,保护内皮功能及肾功能。
