摘要
研究天然药物槟榔碱对培养的牛主动脉内皮细胞氧化损伤时MCP-1和ICAM-1mRNA表达水平的影响,探讨其抗动脉粥样硬化的作用机制。用血管内皮细胞与不同浓度槟榔碱预孵育20h后,给予氧化低密度脂蛋白损伤,提取各组细胞总RNA,通过逆转录-聚合酶链式反应技术,以β2-微球蛋白为内参照,检测内皮细胞MCP-1和ICAM-1mRNA表达水平的变化。得到内皮细胞与槟榔碱预孵育后,其MCP-1和ICAM-1mRNA的表达水平明显低于与氧化低密度脂蛋白直接接触的细胞。说明氧化低密度脂蛋白可诱导牛主动脉内皮细胞MCP-1和ICAM-1mRNA表达水平增加,槟榔碱可以减轻其过量表达,保护内皮细胞。
abstract：To investigate the effectsof arecoline against MCP-1 and ICAM-1 mRNA expression in cultured endothelial cells derived from bovine aorta,the ICAM-1 and MCP-1 mRNA expression in bovine aorta endothelial cells was measured by reverse transcription-polymerase chain reaction and normalized to the mRNA level of /32-microglobulin in the absence or in the presence of ox-LDL or land pivanampeta. It is resulted that both MCP- 1 and ICAM-1 mRNA expression in the endothelial cells derived from bovine aorta were increased by ox-LDL stimulation ,which could be prevented by arecoline at the concentrations of 10 and 100μmol/L. It is conclused that the over expressionof MCP-1 and ICAM-1 in endothelial cells induced by ox-LDL could be blocked by arecoline. Arecoline can protect the endothelial cellsfrom the oxidative injury.
出处
《科学技术与工程》
2007年第12期2780-2783,共4页
Science Technology and Engineering
基金
国家重点基础研究发展规划项目(GT1998051112)资助
