Cassava （Manihot esculenta Crantz） production in Akwa Ibom State is limited by cassava anthracnose disease （CAD） which causes enormous losses in planting materials. Survey of the disease was conducted in the wet a...Cassava （Manihot esculenta Crantz） production in Akwa Ibom State is limited by cassava anthracnose disease （CAD） which causes enormous losses in planting materials. Survey of the disease was conducted in the wet and dry seasons in six major cassava-growing areas （Essien Udim, Ikono, Itu, Nsit Atai, Oruk Anam and Uyo） of the State to determine the incidence and severity of the disease. Ten commonly grown cassava varieties were then screened in the greenhouse by inoculating each variety with the spore suspension of virulent isolate of the cassava anthracnose pathogen. Disease severity was assessed using size of lesions on the inoculated plants and defoliation caused by the infection. Results of the survey showed that Nsit Atai is a high infection area for CAD. It recorded the highest disease incidence and severity in both the wet season （36.59% and 3.52） and dry season （9.26% and 2.20）, respectively. The mean disease incidence （24.10%） and severity （3.23） for the six study areas were higher in the wet season indicating the importance of this season in the occurrence of CAD. Results of the screening test showed that TMS 98/30572 was resistant to the disease. The small lesion size （5.00 mm） observed in this variety would ensure less stem disruption and timely delivery of resources to the actively growing points. The low defoliation （5,65%） observed in the same variety would enhance the photosynthetic capacity of the plant. Therefore, TMS 98/30572 may be recommended to cassava farmers in Akwa Ibom State.展开更多
The class of nucleotide-binding site(NBS)-Leucine-rich repeat(LRR) disease resistance genes play an important role in defending plants from a variety of pathogens and insect pests. Consequently, many NBS-LRR genes hav...The class of nucleotide-binding site(NBS)-Leucine-rich repeat(LRR) disease resistance genes play an important role in defending plants from a variety of pathogens and insect pests. Consequently, many NBS-LRR genes have been identified in various plant species. In this study, we identified 617 NBS-encoding genes in the Medicago truncatula genome(Mt3.5v5) and divided them into two groups, regular(490) and non-regular(127) NBSLRR genes. The regular NBS-LRR genes were characterized on the bases of structural diversity, chromosomal location, gene duplication, conserved protein motifs, and EST expression profiling. According to N-terminal motifs and LRR motifs, the 490 regular NBS-LRR genes were then classified into 10 types: CC-NBS(4), CC-NBS-LRR(212), TIR-NBS(20), TIR-NBS-LRR(160), TIR-NBS-TIR(1), TIR-NBS-TIR-LRR(2), NBS-TIR(7), NBS-TIR-LRR(1), NBS(10), and NBS-LRR(73). Analysis of the physical location and duplications of the regular NBS-LRR genes revealed that the M. truncatula genome is similar to rice. Interestingly, we found that TIR-type genes are more frequently expressed than non-TIR-type genes in M. truncatula, whereas the number of non-TIR-type regular NBSLRR genes was greater than TIR-type genes, suggesting the gene expression was not associated with the total number of NBS-LRR genes. Moreover, we found that the phylogenetic tree supported our division of the regular NBS-LRR genes into two distinct clades(TIR-type and non-TIR-type), but some of the non-TIR-type lineages contain TIR-type genes. These analyses provide a robust database of NBS-LRR genes in M. truncatula that will facilitate the isolation of new resistance genes and breeding strategies to engineer disease resistance in leguminous crop.展开更多
文摘Cassava （Manihot esculenta Crantz） production in Akwa Ibom State is limited by cassava anthracnose disease （CAD） which causes enormous losses in planting materials. Survey of the disease was conducted in the wet and dry seasons in six major cassava-growing areas （Essien Udim, Ikono, Itu, Nsit Atai, Oruk Anam and Uyo） of the State to determine the incidence and severity of the disease. Ten commonly grown cassava varieties were then screened in the greenhouse by inoculating each variety with the spore suspension of virulent isolate of the cassava anthracnose pathogen. Disease severity was assessed using size of lesions on the inoculated plants and defoliation caused by the infection. Results of the survey showed that Nsit Atai is a high infection area for CAD. It recorded the highest disease incidence and severity in both the wet season （36.59% and 3.52） and dry season （9.26% and 2.20）, respectively. The mean disease incidence （24.10%） and severity （3.23） for the six study areas were higher in the wet season indicating the importance of this season in the occurrence of CAD. Results of the screening test showed that TMS 98/30572 was resistant to the disease. The small lesion size （5.00 mm） observed in this variety would ensure less stem disruption and timely delivery of resources to the actively growing points. The low defoliation （5,65%） observed in the same variety would enhance the photosynthetic capacity of the plant. Therefore, TMS 98/30572 may be recommended to cassava farmers in Akwa Ibom State.
基金supported by the National Basic Research Program of China(2014CB138702)
文摘The class of nucleotide-binding site(NBS)-Leucine-rich repeat(LRR) disease resistance genes play an important role in defending plants from a variety of pathogens and insect pests. Consequently, many NBS-LRR genes have been identified in various plant species. In this study, we identified 617 NBS-encoding genes in the Medicago truncatula genome(Mt3.5v5) and divided them into two groups, regular(490) and non-regular(127) NBSLRR genes. The regular NBS-LRR genes were characterized on the bases of structural diversity, chromosomal location, gene duplication, conserved protein motifs, and EST expression profiling. According to N-terminal motifs and LRR motifs, the 490 regular NBS-LRR genes were then classified into 10 types: CC-NBS(4), CC-NBS-LRR(212), TIR-NBS(20), TIR-NBS-LRR(160), TIR-NBS-TIR(1), TIR-NBS-TIR-LRR(2), NBS-TIR(7), NBS-TIR-LRR(1), NBS(10), and NBS-LRR(73). Analysis of the physical location and duplications of the regular NBS-LRR genes revealed that the M. truncatula genome is similar to rice. Interestingly, we found that TIR-type genes are more frequently expressed than non-TIR-type genes in M. truncatula, whereas the number of non-TIR-type regular NBSLRR genes was greater than TIR-type genes, suggesting the gene expression was not associated with the total number of NBS-LRR genes. Moreover, we found that the phylogenetic tree supported our division of the regular NBS-LRR genes into two distinct clades(TIR-type and non-TIR-type), but some of the non-TIR-type lineages contain TIR-type genes. These analyses provide a robust database of NBS-LRR genes in M. truncatula that will facilitate the isolation of new resistance genes and breeding strategies to engineer disease resistance in leguminous crop.
