Daji （Cirsium japonicum） has been applied against gastric disorders, lung diseases, and cardiovascular problems in thetraditional Chinese medicinal system. The present study was to investigate the protective effects...Daji （Cirsium japonicum） has been applied against gastric disorders, lung diseases, and cardiovascular problems in thetraditional Chinese medicinal system. The present study was to investigate the protective effects of Daji （Cirsiumjaponicum） polysaccharide extracts （CJP） against hydrogen peroxide （H2O2） shock in rat H9c2 myocardial cells. First,CJP was isolated by hot water extraction and ethanol precipitation; it was then characterized by high performance liquidchromatography and infrared spectrum analysis. Rat H9c2 cells were subjected to H2O2 treatment to establish a cellinjury model. The 3- （4,5- dimethylthiazol- 2-yl）-2,5- diphenyltetrazolium bromide assay showed that CJP pretreatmentsignificantly ameliorated the H2O2 injury in a dose-dependent manner. Furthermore, the cell apoptosis induced by H2O2was markedly inhibited by CJP pretreatment, whereas the cleavage level of caspase-3, -8, and -9 was reduced. Inaddition, the p38 mitogen-activated protein kinase pathway might be involved in the protective effect of CJP onmyocardial cells. Therefore, we conclude that polysaccharide extracts of Daji （Cirsium japonicum） protect rat H9c2myocardial cells from oxidative stress induced by H2O2.展开更多
This article discusses the role that nature plays in ethnic literature and films in the Seventeen-Year Period(1949-66).It takes the story of Daji and Her Fathers as an example and investigates the ways in which nature...This article discusses the role that nature plays in ethnic literature and films in the Seventeen-Year Period(1949-66).It takes the story of Daji and Her Fathers as an example and investigates the ways in which nature features in the reconstruction of ethnic identity in the formation of a multiethnic nation as in the case of China.I will explore three aspects:first,the debate on humanism,which was closely related to ethnic minority film production at the time.A central issue of the debate then was the question of“humanistic”-that is,affective,emotional,subjective,and most importantly,natural-expression in literary and art works.Ethnic minority identity,with its unique status,was given some latitude for humanistic expression and“natural”understanding.Second,due to ethnic minority groups’special significance in China’s nation-building,a reconstruction of ethnic minority nature became imperative for the People’s Republic of China.This reconstruction involves mostly restructuring a“second nature,”or dialectic nature of minority under the socialist mandate.This dialectic nature demands something more than natural,immediate constituents and requires a socially and politically mediated ethnic minority nature that is aligned with multiethnic nationality.Third,this dialectic nature is to be formed following Marxist dialectical materialism,mainly through the means of social(ist)labor that changes nature.展开更多
文摘Daji （Cirsium japonicum） has been applied against gastric disorders, lung diseases, and cardiovascular problems in thetraditional Chinese medicinal system. The present study was to investigate the protective effects of Daji （Cirsiumjaponicum） polysaccharide extracts （CJP） against hydrogen peroxide （H2O2） shock in rat H9c2 myocardial cells. First,CJP was isolated by hot water extraction and ethanol precipitation; it was then characterized by high performance liquidchromatography and infrared spectrum analysis. Rat H9c2 cells were subjected to H2O2 treatment to establish a cellinjury model. The 3- （4,5- dimethylthiazol- 2-yl）-2,5- diphenyltetrazolium bromide assay showed that CJP pretreatmentsignificantly ameliorated the H2O2 injury in a dose-dependent manner. Furthermore, the cell apoptosis induced by H2O2was markedly inhibited by CJP pretreatment, whereas the cleavage level of caspase-3, -8, and -9 was reduced. Inaddition, the p38 mitogen-activated protein kinase pathway might be involved in the protective effect of CJP onmyocardial cells. Therefore, we conclude that polysaccharide extracts of Daji （Cirsium japonicum） protect rat H9c2myocardial cells from oxidative stress induced by H2O2.
文摘This article discusses the role that nature plays in ethnic literature and films in the Seventeen-Year Period(1949-66).It takes the story of Daji and Her Fathers as an example and investigates the ways in which nature features in the reconstruction of ethnic identity in the formation of a multiethnic nation as in the case of China.I will explore three aspects:first,the debate on humanism,which was closely related to ethnic minority film production at the time.A central issue of the debate then was the question of“humanistic”-that is,affective,emotional,subjective,and most importantly,natural-expression in literary and art works.Ethnic minority identity,with its unique status,was given some latitude for humanistic expression and“natural”understanding.Second,due to ethnic minority groups’special significance in China’s nation-building,a reconstruction of ethnic minority nature became imperative for the People’s Republic of China.This reconstruction involves mostly restructuring a“second nature,”or dialectic nature of minority under the socialist mandate.This dialectic nature demands something more than natural,immediate constituents and requires a socially and politically mediated ethnic minority nature that is aligned with multiethnic nationality.Third,this dialectic nature is to be formed following Marxist dialectical materialism,mainly through the means of social(ist)labor that changes nature.
