The rearrangement of Bcl-1 gene (Bcl-1/IgH rearrangement) and expression of cyclin D1 in multiple myeloma (MM) precursor cells were studied and the role of cyclin D1 in the pathogenesis of MM was investigated. The BCL...The rearrangement of Bcl-1 gene (Bcl-1/IgH rearrangement) and expression of cyclin D1 in multiple myeloma (MM) precursor cells were studied and the role of cyclin D1 in the pathogenesis of MM was investigated. The BCL-1 rearrangement and cyclin D1 protein expression in 15 cases of MM were detected. By using hemi-nested polymerase chain reaction (PCR) the genomic DNA from fresh peripheral blood and bone marrow was amplified and the expression of cyclin D1 in the smears was detected by using immunohistochemical method. Ten volunteer with normal bone marrow served as control group. The results showed Bcl-1 rearrangement was detectable in 3/15 (20 %) MM patients and cyclin D, expression in 4/15 (27 % ) MM patients. BeLl-1 rearrangement and cyclin D1 protein expression were also detected in MM precursor cells. No overexpression of cyclin D1 or the rearrangement of the BeL-1 gene was found in the 10 volunteers. It was concluded that Bel-1 rearrangement and cyclin D1 protein overexpression were detected in MM precursor cells, speculating that overexpression of cyclin D1 protein may play an initial (critical) role in the pathogenesis of MM.展开更多
目的探讨NR3C1基因Bcl-1多态性位点与汉族高原肺水肿(HAPE)相关关系。方法采用病例对照研究,选取HAPE患者133例及性别年龄匹配的对照组135例。采用Sequenom Mass ARRAYSNP检测技术对Bcl-1位点进行基因分型。结果 Bcl-1的基因型GG在HAP...目的探讨NR3C1基因Bcl-1多态性位点与汉族高原肺水肿(HAPE)相关关系。方法采用病例对照研究,选取HAPE患者133例及性别年龄匹配的对照组135例。采用Sequenom Mass ARRAYSNP检测技术对Bcl-1位点进行基因分型。结果 Bcl-1的基因型GG在HAPE组(69.9%)和对照组(56.3%)组间差异有显著性(P<0.05);等位基因G在HAPE组(83.5%)和对照组(74.4%)组间差异有显著性(P<0.05),odds ratio为1.732(1.134~2.645)。结论 Bcl-1的基因型GG和等位基因G在HAPE组和对照组间差异有显著性,可能与HAPE的病理生理过程相关。展开更多
文摘The rearrangement of Bcl-1 gene (Bcl-1/IgH rearrangement) and expression of cyclin D1 in multiple myeloma (MM) precursor cells were studied and the role of cyclin D1 in the pathogenesis of MM was investigated. The BCL-1 rearrangement and cyclin D1 protein expression in 15 cases of MM were detected. By using hemi-nested polymerase chain reaction (PCR) the genomic DNA from fresh peripheral blood and bone marrow was amplified and the expression of cyclin D1 in the smears was detected by using immunohistochemical method. Ten volunteer with normal bone marrow served as control group. The results showed Bcl-1 rearrangement was detectable in 3/15 (20 %) MM patients and cyclin D, expression in 4/15 (27 % ) MM patients. BeLl-1 rearrangement and cyclin D1 protein expression were also detected in MM precursor cells. No overexpression of cyclin D1 or the rearrangement of the BeL-1 gene was found in the 10 volunteers. It was concluded that Bel-1 rearrangement and cyclin D1 protein overexpression were detected in MM precursor cells, speculating that overexpression of cyclin D1 protein may play an initial (critical) role in the pathogenesis of MM.
