摘要
21例多发性骨髓瘤患者的外周血和骨髓的单个核细胞,同时用免疫组织化学和两步法半筑巢式多聚酶链反应(PCR)技术进行分析,检出5例(24%)细胞周期D_1蛋白高表达,4例(19%)bcl-1基因重排.该4例bcl-1基因重排的患者都高表达细胞周期D_1蛋白.5例细胞周期D_1蛋白高表达的患者都具有较晚的临床分期、骨髓中瘤细胞比率较高、化疗效果差等特点.
The rearrangement of bcl-1gene and the expression of cyclin D] protein in peripheral blood and marrow mononuclear cells in 21 multiple myeloma (MM) patients were simultaneously analyzed by using immunohistochemistry method and hemi-nested polymerase chain reaction (PCR). 4/21(19%) MM patients displayed bcl-1 rearrangement by PCR and 5/21 (24%)MM patients showed cyclin D1 overexpression by immunohistochemistry method. All 4 cases of bcl-1 rearrangement had overexpression of cyclin D1 protein, bcl-1 rearrangement and(or)cyclin D1 protein overexpression was a poor prognostic marker for MM patients. Five cases of cyclin D1 protein overexpression were characterized by advanced clinical stage .a highly disseminated tumor,and the refractoriness to standard therapy.
出处
《华中医学杂志》
1999年第5期197-198,221,共3页
Central China Medical Journal
