Background Scavenger receptor that binds phosphatidylserine and oxidized lipoprotein/CXC chemokine ligand 16 （SR-PSOX/CXCL16） promotes foam cell formation through the tumor necrosis factor （TNF）-α mediated mechan...Background Scavenger receptor that binds phosphatidylserine and oxidized lipoprotein/CXC chemokine ligand 16 （SR-PSOX/CXCL16） promotes foam cell formation through the tumor necrosis factor （TNF）-α mediated mechanism. Because chemokine CXCL16 could be expressed in atherosclerotic lesions and induce smooth muscle cell （SMC） proliferation, we＇presume that the monocyte SR-PSOX/CXCL16 detection in the patients＇ peripheral blood will be important for early diagnosis and prognosis of atherosclerosis （AS）. Methods Enrolled in this study were 40 patients with acute coronary syndrome （ACS）, including 20 patients with acute myocardial infarction （AMI） and 20 patients with unstable angina pectoris （UAP）, and 20 normal controls. Monocytes in the peripheral blood were isolated, and the changes of expression of CXCL16/SR-PSOX mRNA were compared using reverse transcription-polymerase chain reaction （RT-PCR）, with β-actin as internal control. We compared the expression of CXCL16/SR-PSOX in the ACS subgroups, using Western-blot to analyze protein expression levels. Tissue sections were made from biopsy specimens taken from patients with infective endocarditis, liver cirrhosis, and lung cancer as well as normal controls. And the expression of CXCL16/SR- PSOX was analyzed with a confocal microscope. Results The expression of CXCL16/SR-PSOX mRNA and protein in the monocytes of peripheral blood was significantly higher in ACS patients than in normal controls （P〈0.05）; however, there was no significant difference in CXCL16/SR-PSOX expression between UAP group and AMI group （P〉0.05）. Immunofluorescence showed that there were low expression of SR-PSOX in normal vascular endothelial cells and enhanced expression in every layer of the infected vessels, while spreading from endothelial cells to surrounding tissues as infection worsens. Confocal microscopy showed that the expression of SR-PSOX was enhanced in the infiltrated lymphocytes in liver cirrhosis, and that the expression level was pr展开更多
Objectives To develop a simple, accurate and reproducible method, which combines macro and histopathological techniques for determining the degree of lipid deposition in genetically modified mice. Method The entire a...Objectives To develop a simple, accurate and reproducible method, which combines macro and histopathological techniques for determining the degree of lipid deposition in genetically modified mice. Method The entire aortas from C57BL/6, ldlr-/- and apoE-/- mice were stained with Sudan IV using either in vivo perfusion or traditional in vitro enface staining techniques. Histological sections of aortic root and hearts were embedded in tissue freezing medium and cut with a cryostat, then stained with Oil Red O. The calculated aortic root area based on the aortic root circumference was used to reduce measurement errors. Results The in vitro en face staining can stain all fat, which include the adventitial tissue around aorta. However the in vivo perfusion staining can specifically stain the fatty deposition inside of aorta. Both entire aorta and aortic root section staining showed that there was a highly significant increase in fatty deposition in the aortas of the genetic modified mice. Although all mice genetic background was same, the apoE-/- mice had larger atherosclerotic lesions than ldlr-/- mice. Conclusions The new in vivo peffusion method is more accurate than the in vitro en face method. The combination of these macro and microscopic techniques overcomes the shortcomings of the earlier published methods which are generally limited to the measurement of fatty red staining areas only, neglecting non-specific adventitial fat staining around aorta and aortic root section tissue distortion.展开更多
目的探讨血清钾水平与冠状动脉病变程度的关联性。方法纳入行冠状动脉造影者246例,按病变累及主要冠状动脉支数分为对照组(0只病变,81例)、单支病变组(43例)、双支病变组(46例)和三支病变组(76例),按照血清钾水平分为低水平血清钾组(低...目的探讨血清钾水平与冠状动脉病变程度的关联性。方法纳入行冠状动脉造影者246例,按病变累及主要冠状动脉支数分为对照组(0只病变,81例)、单支病变组(43例)、双支病变组(46例)和三支病变组(76例),按照血清钾水平分为低水平血清钾组(低钾组,K+<4.0 mmol/L)99例和高水平血清钾组(高钾组,K+≥4.0 mmol/L)147例;采用Gensini评分定量评估各支冠状动脉的狭窄程度;比较各组的临床资料,分析Gensini评分与各临床指标的相关性,采用Logistic回归分析冠心病的危险因素。结果 (1)三支病变组的年龄、男性、吸烟、2型糖尿病、高血压患者比例均高于对照组,三支病变组的血清钾浓度低于对照组和单支病变组(mmol/L:3.97±0.37 vs 4.11±0.33 vs 4.13±0.41),差异均有统计学意义;双支病变组的年龄、男性、高血压病患者比例高于对照组。单支病变组的男性和高血压患者比例高于对照组。各组Gensini评分:三支病变组>双支病变组>单支病变组>对照组(均P<0.05)。(2)低钾组Gensini评分[36(8,94)]高于高钾组[16(0,56)],差异有统计学意义。(3)血清钾与Gensini评分呈负相关(r=-0.206,P=0.001)。(4)高龄、男性、血清钾低水平、合并有糖尿病和高血压为冠心病的危险因素。结论血清钾水平与冠状动脉病变严重程度呈负相关,血清钾低水平为冠心病的独立危险因素。展开更多
目的探讨血压变异性与冠状动脉病变程度的相关性。方法选取我院2015年1月-2016年3月收治的高血压患者109例,均行MSCT冠状动脉造影检查及24 h动态血压监测(24 h ABPM),根据MSCT冠状动脉造影检查结果,将患者分为冠状动脉狭窄组及冠状动脉...目的探讨血压变异性与冠状动脉病变程度的相关性。方法选取我院2015年1月-2016年3月收治的高血压患者109例,均行MSCT冠状动脉造影检查及24 h动态血压监测(24 h ABPM),根据MSCT冠状动脉造影检查结果,将患者分为冠状动脉狭窄组及冠状动脉正常组,分析两组患者血压波动类型与血管段狭窄程度评分(SSS)的相关性。结果两组患者间血压变异性差异无统计学意义(P>0.05);Logistic回归分析结果显示,冠状动脉狭窄与24 h收缩压标准差、白昼收缩压标准差和白昼舒张压标准差存在显著相关性(P<0.001);夜间舒张压标准差与SSS值之间呈正相关性(P<0.001);多重线性回归分析结果显示,患者年龄、24 h舒张压标准差与SSS值呈现正相关性(P<0.05)。结论血压波动类型与冠状动脉病变程度及SSS值密切相关,定期监测血压变异性对冠状动脉病变有一定预测作用。展开更多
基金This work was supported by grants from the National Natural Science Foundation of China （No. 30400265 and 30671047）, Ministry of Education Science and Technology Key Project （No. 207078） and the Youth Foundation of Hunan Province Education Department （No. 06B079）.
文摘Background Scavenger receptor that binds phosphatidylserine and oxidized lipoprotein/CXC chemokine ligand 16 （SR-PSOX/CXCL16） promotes foam cell formation through the tumor necrosis factor （TNF）-α mediated mechanism. Because chemokine CXCL16 could be expressed in atherosclerotic lesions and induce smooth muscle cell （SMC） proliferation, we＇presume that the monocyte SR-PSOX/CXCL16 detection in the patients＇ peripheral blood will be important for early diagnosis and prognosis of atherosclerosis （AS）. Methods Enrolled in this study were 40 patients with acute coronary syndrome （ACS）, including 20 patients with acute myocardial infarction （AMI） and 20 patients with unstable angina pectoris （UAP）, and 20 normal controls. Monocytes in the peripheral blood were isolated, and the changes of expression of CXCL16/SR-PSOX mRNA were compared using reverse transcription-polymerase chain reaction （RT-PCR）, with β-actin as internal control. We compared the expression of CXCL16/SR-PSOX in the ACS subgroups, using Western-blot to analyze protein expression levels. Tissue sections were made from biopsy specimens taken from patients with infective endocarditis, liver cirrhosis, and lung cancer as well as normal controls. And the expression of CXCL16/SR- PSOX was analyzed with a confocal microscope. Results The expression of CXCL16/SR-PSOX mRNA and protein in the monocytes of peripheral blood was significantly higher in ACS patients than in normal controls （P〈0.05）; however, there was no significant difference in CXCL16/SR-PSOX expression between UAP group and AMI group （P〉0.05）. Immunofluorescence showed that there were low expression of SR-PSOX in normal vascular endothelial cells and enhanced expression in every layer of the infected vessels, while spreading from endothelial cells to surrounding tissues as infection worsens. Confocal microscopy showed that the expression of SR-PSOX was enhanced in the infiltrated lymphocytes in liver cirrhosis, and that the expression level was pr
基金supported by the Hong Kong WangKuan Cheng Foundation GrantBritish Heart Foundation
文摘Objectives To develop a simple, accurate and reproducible method, which combines macro and histopathological techniques for determining the degree of lipid deposition in genetically modified mice. Method The entire aortas from C57BL/6, ldlr-/- and apoE-/- mice were stained with Sudan IV using either in vivo perfusion or traditional in vitro enface staining techniques. Histological sections of aortic root and hearts were embedded in tissue freezing medium and cut with a cryostat, then stained with Oil Red O. The calculated aortic root area based on the aortic root circumference was used to reduce measurement errors. Results The in vitro en face staining can stain all fat, which include the adventitial tissue around aorta. However the in vivo perfusion staining can specifically stain the fatty deposition inside of aorta. Both entire aorta and aortic root section staining showed that there was a highly significant increase in fatty deposition in the aortas of the genetic modified mice. Although all mice genetic background was same, the apoE-/- mice had larger atherosclerotic lesions than ldlr-/- mice. Conclusions The new in vivo peffusion method is more accurate than the in vitro en face method. The combination of these macro and microscopic techniques overcomes the shortcomings of the earlier published methods which are generally limited to the measurement of fatty red staining areas only, neglecting non-specific adventitial fat staining around aorta and aortic root section tissue distortion.
文摘目的探讨血清钾水平与冠状动脉病变程度的关联性。方法纳入行冠状动脉造影者246例,按病变累及主要冠状动脉支数分为对照组(0只病变,81例)、单支病变组(43例)、双支病变组(46例)和三支病变组(76例),按照血清钾水平分为低水平血清钾组(低钾组,K+<4.0 mmol/L)99例和高水平血清钾组(高钾组,K+≥4.0 mmol/L)147例;采用Gensini评分定量评估各支冠状动脉的狭窄程度;比较各组的临床资料,分析Gensini评分与各临床指标的相关性,采用Logistic回归分析冠心病的危险因素。结果 (1)三支病变组的年龄、男性、吸烟、2型糖尿病、高血压患者比例均高于对照组,三支病变组的血清钾浓度低于对照组和单支病变组(mmol/L:3.97±0.37 vs 4.11±0.33 vs 4.13±0.41),差异均有统计学意义;双支病变组的年龄、男性、高血压病患者比例高于对照组。单支病变组的男性和高血压患者比例高于对照组。各组Gensini评分:三支病变组>双支病变组>单支病变组>对照组(均P<0.05)。(2)低钾组Gensini评分[36(8,94)]高于高钾组[16(0,56)],差异有统计学意义。(3)血清钾与Gensini评分呈负相关(r=-0.206,P=0.001)。(4)高龄、男性、血清钾低水平、合并有糖尿病和高血压为冠心病的危险因素。结论血清钾水平与冠状动脉病变严重程度呈负相关,血清钾低水平为冠心病的独立危险因素。
