AIM: To reveal the correlation between the functional differentiation phenotypes of gastric carcinoma cells and the invasion and metastasis by a new way of cell-function classification.METHODS:Surgically resected spec...AIM: To reveal the correlation between the functional differentiation phenotypes of gastric carcinoma cells and the invasion and metastasis by a new way of cell-function classification.METHODS:Surgically resected specimens of 361 gastric carcinomas(GC) were investigated with enzyme-, mucin-, and tumor-related marker immunohistochemistry. According to the direction of cell-function differentiation, stomach carcinomas were divided into five functionally differentiated types. RESULTS: (1) Absorptive function differentiation type (AFDT): there were 82 (22.7%) patients including 76 (92.7%) aged 45 years. Sixty-nine (84.1%) cases belonged to the intestinal type. Thirty-eight (46.3%) expressed CD44v6 and 9 (13.6%) of 66 male patients developed liver metastasis.The 5-year survival rate of patients in this group (58.5%) was higher than those with the other types (P【0.01). (2) Mucin secreting function differentiation type (MSFDT): 54 (15%) cases. Fifty-three (98.1%) tumors had penetrated the serosa, 12 (22.2%) expressed ER and 22 (40.7%) expressed CD44v6. The postoperative 5-year survival rate was 28.6%. (3) Absorptive and mucin-producing function differentiation type (AMPFDT): there were 180 (49.9%) cases, including 31 (17.2%) aged younger than 45 years. The tumor was more common in women (62, 34.4%,) and expressed more frequently estrogen receptors (ER) (129, 81.7%) than other types (P【0.01). Ovary metastasis was found in 12 (19.4%) out of 62 female subjects. The patients with this type GC had the lowest 5-year survival rate (24.7%) among all types. (4) Specific function differentiation type (SFDT): 13 (3.6%) cases. Nine (69.2%) tumors of this type derived from APUD system, the other 4 (30.7%) were of different histological differentiation. Sixty per cent of the patients survived at least five years. (5) Non-function differentiation type (NFDT): 32 (8.9%) cases. Nineteen (59.4%) cases had lymph node metastases but no one with liver or ovary metastasis. The 5-year survival rate was 28.1%. CONCLUSION: This new cell-f展开更多
目的:探讨乳腺癌转移抑制基因(breast cancer metastasis suppressor 1,BRMS1)mRNA和CD44V6mRNA的异常表达与子宫内膜癌侵袭及转移的关系。方法:采用逆转录多聚酶链反应(RT-PCR)法,检测42例子宫内膜癌、12例子宫内膜不典型增生和15例正...目的:探讨乳腺癌转移抑制基因(breast cancer metastasis suppressor 1,BRMS1)mRNA和CD44V6mRNA的异常表达与子宫内膜癌侵袭及转移的关系。方法:采用逆转录多聚酶链反应(RT-PCR)法,检测42例子宫内膜癌、12例子宫内膜不典型增生和15例正常子宫内膜组织中BRMS1mRNA和CD44V6mRNA的表达情况,分析其与各种临床参数的关系。结果:1)子宫内膜癌患者的BRMS1基因表达率为37·5%,明显低于不典型增生的75·0%和正常内膜的86·7%;与临床分期(χ2=4·978)、肌层浸润深度(χ2=5·999)及淋巴结转移(χ2=4·200)均密切相关,P值均<0·05;2)子宫内膜癌患者的CD44V6基因表达率为69·0%,显著高于不典型增生的16·7%和正常内膜的0,P<0·01;与临床分期(χ2=5·802)及淋巴结转移(χ2=5·570)均密切相关,P值均<0·05;3)BRMS1与CD44V6mRNA的阳性表达率之间无相关关系,肯德尔相关系数为0·069,P>0·05。结论:BRMS1和CD44V6mRNA的异常表达是子宫内膜癌发生发展、浸润转移的重要分子学改变,同时检测两者可作为临床预测和评估其侵袭及转移的重要参考指标。展开更多
基金Project supported by the National Natural Science Foundation of China, No. 39270300. No. 39370772Training Program for Trans-Century Talents by the State Education Commission of China
文摘AIM: To reveal the correlation between the functional differentiation phenotypes of gastric carcinoma cells and the invasion and metastasis by a new way of cell-function classification.METHODS:Surgically resected specimens of 361 gastric carcinomas(GC) were investigated with enzyme-, mucin-, and tumor-related marker immunohistochemistry. According to the direction of cell-function differentiation, stomach carcinomas were divided into five functionally differentiated types. RESULTS: (1) Absorptive function differentiation type (AFDT): there were 82 (22.7%) patients including 76 (92.7%) aged 45 years. Sixty-nine (84.1%) cases belonged to the intestinal type. Thirty-eight (46.3%) expressed CD44v6 and 9 (13.6%) of 66 male patients developed liver metastasis.The 5-year survival rate of patients in this group (58.5%) was higher than those with the other types (P【0.01). (2) Mucin secreting function differentiation type (MSFDT): 54 (15%) cases. Fifty-three (98.1%) tumors had penetrated the serosa, 12 (22.2%) expressed ER and 22 (40.7%) expressed CD44v6. The postoperative 5-year survival rate was 28.6%. (3) Absorptive and mucin-producing function differentiation type (AMPFDT): there were 180 (49.9%) cases, including 31 (17.2%) aged younger than 45 years. The tumor was more common in women (62, 34.4%,) and expressed more frequently estrogen receptors (ER) (129, 81.7%) than other types (P【0.01). Ovary metastasis was found in 12 (19.4%) out of 62 female subjects. The patients with this type GC had the lowest 5-year survival rate (24.7%) among all types. (4) Specific function differentiation type (SFDT): 13 (3.6%) cases. Nine (69.2%) tumors of this type derived from APUD system, the other 4 (30.7%) were of different histological differentiation. Sixty per cent of the patients survived at least five years. (5) Non-function differentiation type (NFDT): 32 (8.9%) cases. Nineteen (59.4%) cases had lymph node metastases but no one with liver or ovary metastasis. The 5-year survival rate was 28.1%. CONCLUSION: This new cell-f
文摘目的:探讨乳腺癌转移抑制基因(breast cancer metastasis suppressor 1,BRMS1)mRNA和CD44V6mRNA的异常表达与子宫内膜癌侵袭及转移的关系。方法:采用逆转录多聚酶链反应(RT-PCR)法,检测42例子宫内膜癌、12例子宫内膜不典型增生和15例正常子宫内膜组织中BRMS1mRNA和CD44V6mRNA的表达情况,分析其与各种临床参数的关系。结果:1)子宫内膜癌患者的BRMS1基因表达率为37·5%,明显低于不典型增生的75·0%和正常内膜的86·7%;与临床分期(χ2=4·978)、肌层浸润深度(χ2=5·999)及淋巴结转移(χ2=4·200)均密切相关,P值均<0·05;2)子宫内膜癌患者的CD44V6基因表达率为69·0%,显著高于不典型增生的16·7%和正常内膜的0,P<0·01;与临床分期(χ2=5·802)及淋巴结转移(χ2=5·570)均密切相关,P值均<0·05;3)BRMS1与CD44V6mRNA的阳性表达率之间无相关关系,肯德尔相关系数为0·069,P>0·05。结论:BRMS1和CD44V6mRNA的异常表达是子宫内膜癌发生发展、浸润转移的重要分子学改变,同时检测两者可作为临床预测和评估其侵袭及转移的重要参考指标。
