Structural variations(SVs)are a feature of plant genomes that has been largely unexplored despite their significant impact on plant phenotypic traits and local adaptation to abiotic and biotic stress.In this study,we ...Structural variations(SVs)are a feature of plant genomes that has been largely unexplored despite their significant impact on plant phenotypic traits and local adaptation to abiotic and biotic stress.In this study,we employed woolly grape(Vitis retordii),a species native to the tropical and subtropical regions of East Asia with both coastal and inland habitats,as a valuable model for examining the impact of SVs on local adaptation.We assembled a haplotype-resolved chromosomal reference genome for woolly grape,and conducted population genetic analyses based on whole-genome sequencing(WGS)data from coastal and inland populations.The demographic analyses revealed recent bottlenecks in all populations and asymmetric gene flow from the inland to the coastal population.In total,1,035 genes associated with plant adaptive regulation for salt stress,radiation,and environmental adaptation were detected underlying local selection by SVs and SNPs in the coastal population,of which 37.29% and 65.26% were detected by SVs and SNPs,respectively.Candidate genes such as FSD2,RGA1,and AAP8 associated with salt tolerance were found to be highly differentiated and selected during the process of local adaptation to coastal habitats in SV regions.Our study highlights the importance of SVs in local adaptation;candidate genes related to salt stress and climatic adaptation to tropical and subtropical environments are important genomic resources for future breeding programs of grapevine and its rootstocks.展开更多
Hypofractionated radiation therapy has proven effective on locoregional control and tolerance in the adjuvant treatment of breast cancer. The aim of this study is to compare the results of hypofractionated radiation t...Hypofractionated radiation therapy has proven effective on locoregional control and tolerance in the adjuvant treatment of breast cancer. The aim of this study is to compare the results of hypofractionated radiation therapy versus conventional radiation therapy in terms of local control and tolerance. It was a retrospective study of patients observations collected from January 2007 to December 2008 in Department of Radiation Therapy in Institut National d’Oncologie de Rabat. The treatment results were evaluated by the rate of locoregional recurrence, distant recurrence and research of late toxicities. Radiotherapy was delivered using the same technique in both groups, by gamma photons of cobalt 60 with an energy of 1.25 MeV. They were 2 groups: the first group treated with standard dose rate and the second group treated by hypofractionated radiation therapy. The mean age of the patients was 42.8 ± 6.9 years old in the standard group and 43.22 ± 7.2 years old in the hypofractionation group. We noted a predominance of infiltrating ductal carcinoma. The majority of patients were pT<sub>2</sub>, pN<sub>0</sub> and pN<sub>1</sub>.<sub> </sub>The majority of patients had radical surgery and chemotherapy with anthracyclines in both groups. We noted a statistically significant difference in the irradiation of chest wall between the standard (89.2%) and hypofractionated group (70.3%), with p = 0.043. The median duration of radiation therapy was statistically different in both groups: 39 days in the standard and 23 days in the hypofractionated group (p 0.001). The local recurrences were statistically identical to 12 and 24 months (p = 0.999). Concerning toxicities, the frequency of adverse event was similar in both groups. Hypofractionated radiation therapy with a total dose of 42 Gy at 2.8 Gy per fraction in 5 fractions weekly is comparable to standard radiotherapy in terms of local control and tolerance and is therefore a very good alternative to standard treatment.展开更多
Physiological thermotolerance and behavioral thermoregulation are central to seasonal cold adaptation in ectothermic organisms.For species with enhanced mobility,behavioral responses may be of greater importance in th...Physiological thermotolerance and behavioral thermoregulation are central to seasonal cold adaptation in ectothermic organisms.For species with enhanced mobility,behavioral responses may be of greater importance in the cold stress response.Employing the carabid beetles as a study organism,the current study compared physiological thermotolerance and behavioral thermoregulation in carabid species inhabiting cereal fields in different landscape contexts,from fine grain heterogeneous“complex”landscapes to homogenous“simple”landscapes.Physiological thermotolerance was determined via measurement of the CTmin and chill coma temperature.Behavioral responses to cold temperature exposure were determined employing a purpose built arena,and thoracic temperature measured to estimate the efficacy of the behavior as a form of behavioral thermoregulation.Results revealed an influence of landscape composition on the cold tolerance of carabid beetles,although species differed in their sensitivity to landscape intensification.A reduced effect of landscape on the thermotolerance of larger carabid beetles was observed,thought to be the consequence of greater mobility preventing local acclimation to microclimatic variation along the landscape intensification gradient.Investigation into behavioral thermoregulation of the 3 largest species revealed burrowing behavior to be the main behavioral response to cold stress,acting to significantly raise carabid body temperature.This finding highlights the importance of behavioral thermoregulation as a strategy to evade cold stress.The use of behavioral thermoregulation may negate the need to invest in physiological thermotolerance,further offering explanation for the lack of landscape effect on the physiological thermotolerance of larger carabids.展开更多
An automobile test controlling and managing system with computer network is introduced. The architecture of the local network, hardware structure, software structure, design of the test process, and error tolerating r...An automobile test controlling and managing system with computer network is introduced. The architecture of the local network, hardware structure, software structure, design of the test process, and error tolerating redundant design in work position substituting are presented. At last, the pivotal questions solved are discussed. With an advanced structure, this system is multi functional and flexible. Furthermore, advanced computer technology is adopted to improve its technical degree.展开更多
基金supported by the Science Fund Program for Distinguished Young Scholars of the National Natural Science Foundation of China(Overseas)to Yongfeng ZhouNational Natural Science Foundation of China(Nos.32300191,32372662)+1 种基金Guangxi University,Bama Institute of Integration of Industry and Education,postgraduate joint training project(Project Nos.20210020,20210039)the National Key Research and Development Program of China(grants 2023YFF1000100 and 2023YFD2200700).
文摘Structural variations(SVs)are a feature of plant genomes that has been largely unexplored despite their significant impact on plant phenotypic traits and local adaptation to abiotic and biotic stress.In this study,we employed woolly grape(Vitis retordii),a species native to the tropical and subtropical regions of East Asia with both coastal and inland habitats,as a valuable model for examining the impact of SVs on local adaptation.We assembled a haplotype-resolved chromosomal reference genome for woolly grape,and conducted population genetic analyses based on whole-genome sequencing(WGS)data from coastal and inland populations.The demographic analyses revealed recent bottlenecks in all populations and asymmetric gene flow from the inland to the coastal population.In total,1,035 genes associated with plant adaptive regulation for salt stress,radiation,and environmental adaptation were detected underlying local selection by SVs and SNPs in the coastal population,of which 37.29% and 65.26% were detected by SVs and SNPs,respectively.Candidate genes such as FSD2,RGA1,and AAP8 associated with salt tolerance were found to be highly differentiated and selected during the process of local adaptation to coastal habitats in SV regions.Our study highlights the importance of SVs in local adaptation;candidate genes related to salt stress and climatic adaptation to tropical and subtropical environments are important genomic resources for future breeding programs of grapevine and its rootstocks.
文摘Hypofractionated radiation therapy has proven effective on locoregional control and tolerance in the adjuvant treatment of breast cancer. The aim of this study is to compare the results of hypofractionated radiation therapy versus conventional radiation therapy in terms of local control and tolerance. It was a retrospective study of patients observations collected from January 2007 to December 2008 in Department of Radiation Therapy in Institut National d’Oncologie de Rabat. The treatment results were evaluated by the rate of locoregional recurrence, distant recurrence and research of late toxicities. Radiotherapy was delivered using the same technique in both groups, by gamma photons of cobalt 60 with an energy of 1.25 MeV. They were 2 groups: the first group treated with standard dose rate and the second group treated by hypofractionated radiation therapy. The mean age of the patients was 42.8 ± 6.9 years old in the standard group and 43.22 ± 7.2 years old in the hypofractionation group. We noted a predominance of infiltrating ductal carcinoma. The majority of patients were pT<sub>2</sub>, pN<sub>0</sub> and pN<sub>1</sub>.<sub> </sub>The majority of patients had radical surgery and chemotherapy with anthracyclines in both groups. We noted a statistically significant difference in the irradiation of chest wall between the standard (89.2%) and hypofractionated group (70.3%), with p = 0.043. The median duration of radiation therapy was statistically different in both groups: 39 days in the standard and 23 days in the hypofractionated group (p 0.001). The local recurrences were statistically identical to 12 and 24 months (p = 0.999). Concerning toxicities, the frequency of adverse event was similar in both groups. Hypofractionated radiation therapy with a total dose of 42 Gy at 2.8 Gy per fraction in 5 fractions weekly is comparable to standard radiotherapy in terms of local control and tolerance and is therefore a very good alternative to standard treatment.
基金funded by a Marie Sktodowska-Curie Actions Intra-European Fellowship for the project“Climland”(FP7-PEOPLE-2012-IEF-326943)awarded to L.Alford,F.Burel,and J.van Baarenan Individual Fellowship for the project“FAB"(H2020-MSCA-IF-2018-841952)awarded to L.Alford and J.van Baaren.
文摘Physiological thermotolerance and behavioral thermoregulation are central to seasonal cold adaptation in ectothermic organisms.For species with enhanced mobility,behavioral responses may be of greater importance in the cold stress response.Employing the carabid beetles as a study organism,the current study compared physiological thermotolerance and behavioral thermoregulation in carabid species inhabiting cereal fields in different landscape contexts,from fine grain heterogeneous“complex”landscapes to homogenous“simple”landscapes.Physiological thermotolerance was determined via measurement of the CTmin and chill coma temperature.Behavioral responses to cold temperature exposure were determined employing a purpose built arena,and thoracic temperature measured to estimate the efficacy of the behavior as a form of behavioral thermoregulation.Results revealed an influence of landscape composition on the cold tolerance of carabid beetles,although species differed in their sensitivity to landscape intensification.A reduced effect of landscape on the thermotolerance of larger carabid beetles was observed,thought to be the consequence of greater mobility preventing local acclimation to microclimatic variation along the landscape intensification gradient.Investigation into behavioral thermoregulation of the 3 largest species revealed burrowing behavior to be the main behavioral response to cold stress,acting to significantly raise carabid body temperature.This finding highlights the importance of behavioral thermoregulation as a strategy to evade cold stress.The use of behavioral thermoregulation may negate the need to invest in physiological thermotolerance,further offering explanation for the lack of landscape effect on the physiological thermotolerance of larger carabids.
文摘An automobile test controlling and managing system with computer network is introduced. The architecture of the local network, hardware structure, software structure, design of the test process, and error tolerating redundant design in work position substituting are presented. At last, the pivotal questions solved are discussed. With an advanced structure, this system is multi functional and flexible. Furthermore, advanced computer technology is adopted to improve its technical degree.
