BACKGROUND About one-third of refractory irritable bowel syndrome(IBS)cases are caused by gastrointestinal(GI)infection/inflammation,known as post-infectious/postinflammatory IBS(PI-IBS).Although it is known that inte...BACKGROUND About one-third of refractory irritable bowel syndrome(IBS)cases are caused by gastrointestinal(GI)infection/inflammation,known as post-infectious/postinflammatory IBS(PI-IBS).Although it is known that intestinal microbiota and host NOD-like receptor family pyrin domain containing 6(NLRP6)inflammsome signaling are closely related to PI-IBS and moxibustion has a therapeutic effect on PI-IBS,whether moxibustion regulates the intestinal flora and host NLRP6 events in PI-IBS remains unclear.AIM To examine the regulatory effect of moxibustion on intestinal microbiota and host NLRP6 inflammatory signaling in PI-IBS.METHODS Sprague-Dawley rats were divided into a normal control group,a model control group,a mild moxibustion group,and a sham mild moxibustion group.PI-IBS rats in the mild moxibustion group were treated with moxibusiton at bilateral Tianshu(ST 25)and Zusanli(ST36)for 7 consecutive days for 10 min each time.The sham group rats were given the same treatment as the mild moxibustion group except the moxa stick was not ignited.Abdominal withdrawal reflex(AWR)score was measured to assess the visceral sensitivity,and colon histopathology and ultrastructure,colonic myeloperoxidase(MPO)activity,and serum C-reactive protein(CRP)level were measured to evaluate low-grade colonic inflammation in rats.The relative abundance of selected intestinal bacteria in rat feces was detected by 16S rDNA PCR and the NLRP6 inflammsome signaling in the colon was detected by immunofluorescence,qRTPCR,and Western blot.RESULTS The AWR score was significantly decreased and the low-grade intestinal inflammation reflected by serum CRP and colonic MPO levels was inhibited in the mild moxibustion group compared with the sham group.Mild moxibustion remarkably increased the relative DNA abundances of Lactobacillus,Bifidobacterium,and Faecalibacterium prausnitzii but decreased that of Escherichia coli in the gut of PI-IBS rats.Additionally,mild moxibustion induced mRNA and protein expression of intestine lectin 1 but inhibited the ex展开更多
高密度、集约化的凡纳滨对虾养殖常伴随病害的发生,但还不能确定能否利用对虾的肠道细菌差异来指示其健康状况.因此,我们分别采集了健康和发病养殖塘水样和对虾样品,利用Illumina Mi Seq测序技术测定细菌16S rRNA基因,研究细菌的群落结...高密度、集约化的凡纳滨对虾养殖常伴随病害的发生,但还不能确定能否利用对虾的肠道细菌差异来指示其健康状况.因此,我们分别采集了健康和发病养殖塘水样和对虾样品,利用Illumina Mi Seq测序技术测定细菌16S rRNA基因,研究细菌的群落结构和多样性.结果表明:细菌群落结构变异主要受水体中亚硝酸盐、叶绿素a和磷酸盐的影响.发病对虾肠道细菌群落多样性显著低于健康对虾.在健康和发病对虾肠道细菌中,运用响应比分析,我们筛选到了28个丰度差异显著的分类操作单元(OTUs),其中来源于放线菌纲、黄杆菌纲和芽孢杆菌纲的OTUs丰度在发病对虾肠道中显著降低,而隶属于梭状芽孢杆菌纲的OTUs丰度在发病对虾肠道显著增加.此外,我们挑选出61个指示物种,主要属于拟杆菌门、变形菌门、厚壁菌门、浮霉菌门和放线菌门.值得注意的是,它们能够区分样品的来源(水样或虾肠道)和健康状态.本研究为对虾病害预测和益生菌开发提供了理论依据.展开更多
基金Supported by the National Natural Science Foundation of China,No.81503656the National Key Basic Research Program of China(973 Program),No.2015CB554501 and No.2009CB522900
文摘BACKGROUND About one-third of refractory irritable bowel syndrome(IBS)cases are caused by gastrointestinal(GI)infection/inflammation,known as post-infectious/postinflammatory IBS(PI-IBS).Although it is known that intestinal microbiota and host NOD-like receptor family pyrin domain containing 6(NLRP6)inflammsome signaling are closely related to PI-IBS and moxibustion has a therapeutic effect on PI-IBS,whether moxibustion regulates the intestinal flora and host NLRP6 events in PI-IBS remains unclear.AIM To examine the regulatory effect of moxibustion on intestinal microbiota and host NLRP6 inflammatory signaling in PI-IBS.METHODS Sprague-Dawley rats were divided into a normal control group,a model control group,a mild moxibustion group,and a sham mild moxibustion group.PI-IBS rats in the mild moxibustion group were treated with moxibusiton at bilateral Tianshu(ST 25)and Zusanli(ST36)for 7 consecutive days for 10 min each time.The sham group rats were given the same treatment as the mild moxibustion group except the moxa stick was not ignited.Abdominal withdrawal reflex(AWR)score was measured to assess the visceral sensitivity,and colon histopathology and ultrastructure,colonic myeloperoxidase(MPO)activity,and serum C-reactive protein(CRP)level were measured to evaluate low-grade colonic inflammation in rats.The relative abundance of selected intestinal bacteria in rat feces was detected by 16S rDNA PCR and the NLRP6 inflammsome signaling in the colon was detected by immunofluorescence,qRTPCR,and Western blot.RESULTS The AWR score was significantly decreased and the low-grade intestinal inflammation reflected by serum CRP and colonic MPO levels was inhibited in the mild moxibustion group compared with the sham group.Mild moxibustion remarkably increased the relative DNA abundances of Lactobacillus,Bifidobacterium,and Faecalibacterium prausnitzii but decreased that of Escherichia coli in the gut of PI-IBS rats.Additionally,mild moxibustion induced mRNA and protein expression of intestine lectin 1 but inhibited the ex
文摘高密度、集约化的凡纳滨对虾养殖常伴随病害的发生,但还不能确定能否利用对虾的肠道细菌差异来指示其健康状况.因此,我们分别采集了健康和发病养殖塘水样和对虾样品,利用Illumina Mi Seq测序技术测定细菌16S rRNA基因,研究细菌的群落结构和多样性.结果表明:细菌群落结构变异主要受水体中亚硝酸盐、叶绿素a和磷酸盐的影响.发病对虾肠道细菌群落多样性显著低于健康对虾.在健康和发病对虾肠道细菌中,运用响应比分析,我们筛选到了28个丰度差异显著的分类操作单元(OTUs),其中来源于放线菌纲、黄杆菌纲和芽孢杆菌纲的OTUs丰度在发病对虾肠道中显著降低,而隶属于梭状芽孢杆菌纲的OTUs丰度在发病对虾肠道显著增加.此外,我们挑选出61个指示物种,主要属于拟杆菌门、变形菌门、厚壁菌门、浮霉菌门和放线菌门.值得注意的是,它们能够区分样品的来源(水样或虾肠道)和健康状态.本研究为对虾病害预测和益生菌开发提供了理论依据.
