Objective To investigate whether acute dipterex poisoning (ADP) may cause oxidative stress and free radical damage in the bodies of acute dipterex poisoning patients (ADPPs), and to explore the mechanisms by which ADP...Objective To investigate whether acute dipterex poisoning (ADP) may cause oxidative stress and free radical damage in the bodies of acute dipterex poisoning patients (ADPPs), and to explore the mechanisms by which ADP may cause oxidative stress and free radical damage. Methods Fifty ADPPs and fifty healthy adult volunteers (HAVs) whose ages, gender and others were matched with the ADPPs were enrolled in a randomized controlled study, in which concentrations of nitric oxide (NO), vitamin C (VC), vitamin E (VE) and P-carotene (P-CAR) in plasma as well as concentration of lipoperoxide (LPO), and activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and acetylcholinesterase (AChE) in erythrocytes were determined by spectrophotometric analytical methods. Results Compared with the average values of experimental parameters in the HAVs group, the average values of plasma NO and erythrocyte LPO in the ADPPs group were significantly increased (P<0.0001), while those of plasma VC, VE and P-CAR as well as erythrocyte SOD, CAT, GPX and AChE in the ADPPs group were significantly decreased (P<0.0001). Bivariate correlation analysis and partial correlation analysis suggested that when NO and LPO values were increased, and VC, VE, β-CAR, SOD, CAT and GPX values were decreased in the ADPPs, AChE value was decreased gradually in the ADPPs (P<0.001-0.0001). Reliability analysis of experimental parameters reflecting oxidative stress and free radical damage in the ADPPs showed that the reliability coefficient (8 items) alpha=0.6909, and the standardized item alpha=0.8574. Conclusion The findings in the present study suggest that ADP can cause oxidative stress and free radical damage, and inhibit markedly erythrocyte acetylcholinesterase activity in ADPPs.展开更多
研究了5种常用水产药物对白斑狗(Esox Lucius Linnaeus)鱼幼鱼的急性毒性。使用常温静水试验法,探讨了溴氯海因、高锰酸钾、氯化钠、敌百虫和氯氰菊酯5种常用水产药物对白斑狗鱼幼鱼的急性毒性效应,分别在试验24h、48h和96h后记录白斑...研究了5种常用水产药物对白斑狗(Esox Lucius Linnaeus)鱼幼鱼的急性毒性。使用常温静水试验法,探讨了溴氯海因、高锰酸钾、氯化钠、敌百虫和氯氰菊酯5种常用水产药物对白斑狗鱼幼鱼的急性毒性效应,分别在试验24h、48h和96h后记录白斑狗鱼幼鱼的死亡尾数和试验药液的安全质量浓度。结果表明,5种药物的安全浓度分别为1.10mg·L-1、1.37mg·L-1、3309mg·L-1、0.57mg·L-1和0.0080mg·L-1,这5种药物对白斑狗鱼幼鱼毒性依次顺序为氯氰菊酯>敌百虫>溴氯海因>高锰酸钾>氯化钠。展开更多
文摘Objective To investigate whether acute dipterex poisoning (ADP) may cause oxidative stress and free radical damage in the bodies of acute dipterex poisoning patients (ADPPs), and to explore the mechanisms by which ADP may cause oxidative stress and free radical damage. Methods Fifty ADPPs and fifty healthy adult volunteers (HAVs) whose ages, gender and others were matched with the ADPPs were enrolled in a randomized controlled study, in which concentrations of nitric oxide (NO), vitamin C (VC), vitamin E (VE) and P-carotene (P-CAR) in plasma as well as concentration of lipoperoxide (LPO), and activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and acetylcholinesterase (AChE) in erythrocytes were determined by spectrophotometric analytical methods. Results Compared with the average values of experimental parameters in the HAVs group, the average values of plasma NO and erythrocyte LPO in the ADPPs group were significantly increased (P<0.0001), while those of plasma VC, VE and P-CAR as well as erythrocyte SOD, CAT, GPX and AChE in the ADPPs group were significantly decreased (P<0.0001). Bivariate correlation analysis and partial correlation analysis suggested that when NO and LPO values were increased, and VC, VE, β-CAR, SOD, CAT and GPX values were decreased in the ADPPs, AChE value was decreased gradually in the ADPPs (P<0.001-0.0001). Reliability analysis of experimental parameters reflecting oxidative stress and free radical damage in the ADPPs showed that the reliability coefficient (8 items) alpha=0.6909, and the standardized item alpha=0.8574. Conclusion The findings in the present study suggest that ADP can cause oxidative stress and free radical damage, and inhibit markedly erythrocyte acetylcholinesterase activity in ADPPs.
文摘研究了5种常用水产药物对白斑狗(Esox Lucius Linnaeus)鱼幼鱼的急性毒性。使用常温静水试验法,探讨了溴氯海因、高锰酸钾、氯化钠、敌百虫和氯氰菊酯5种常用水产药物对白斑狗鱼幼鱼的急性毒性效应,分别在试验24h、48h和96h后记录白斑狗鱼幼鱼的死亡尾数和试验药液的安全质量浓度。结果表明,5种药物的安全浓度分别为1.10mg·L-1、1.37mg·L-1、3309mg·L-1、0.57mg·L-1和0.0080mg·L-1,这5种药物对白斑狗鱼幼鱼毒性依次顺序为氯氰菊酯>敌百虫>溴氯海因>高锰酸钾>氯化钠。
