目的:观察隔药饼灸对功能性消化不良(FD)肝郁脾虚模型大鼠HPA轴中枢相关受体及激素的影响,探讨隔药饼灸治疗FD可能的作用机制。方法:将SD大鼠随机分为空白(A)组、模型(B)组、隔药饼灸(C)组、艾炷灸(D)组和逍遥散(E)组,每组10只,除A组外...目的:观察隔药饼灸对功能性消化不良(FD)肝郁脾虚模型大鼠HPA轴中枢相关受体及激素的影响,探讨隔药饼灸治疗FD可能的作用机制。方法:将SD大鼠随机分为空白(A)组、模型(B)组、隔药饼灸(C)组、艾炷灸(D)组和逍遥散(E)组,每组10只,除A组外,其余4组采用复合病因造模法造模,连续3周;再按被试因素施加方法,分别进行隔药饼灸、艾炷灸和逍遥散灌胃治疗14d;实验结束后采用RT-PCR检测下丘脑、垂体糖皮质激素受体(GR)m RNA表达,ELISA检测下丘脑促肾上腺皮质激素释放激素(CRH)、垂体促肾上腺皮质激素(ACTH)及肾上腺皮质酮(CORT)的含量。结果:与A组比较,B组大鼠GR m RNA在下丘脑及垂体的表达显著下降(P<0.01),且下丘脑CRH、垂体ACTH、肾上腺CORT含量显著上升(P<0.01);与B组比较,C、D、E组治疗组大鼠GR m RNA在下丘脑及垂体的表达显著上升(P<0.01),且下丘脑CRH、垂体ACTH、肾上腺CORT含量均显著下降(P<0.01);与E组比较,C组大鼠GR m RNA在下丘脑及垂体的表达及下丘脑CRH、垂体ACTH、肾上腺CORT含量相当;而D组大鼠GR m RNA在下丘脑及垂体的表达较C、E组较低(P<0.01),且下丘脑CRH、垂体ACTH、肾上腺CORT含量较C、E组较高(P<0.05)。结论:隔药饼灸能显著上调FD模型大鼠下丘脑、垂体GR m RNA表达,下调其下丘脑CRH、垂体ACTH、肾上腺CORT含量,其作用与逍遥散相当,优于艾炷灸。通过纠正HPA轴中枢相关受体及激素的异常表达,抑制HPA轴功能亢进,改善胃肠动力,这可能是隔药饼灸法治疗FD的另一作用机制。展开更多
目的检测先兆早产患者外周血促肾上腺皮质激素释放激素（CRH）浓度,探讨其浓度与先兆早产症状的相关性及影响其浓度的因素,以探讨CRH浓度作为预测早产指标的可行性。方法选取先兆早产住院孕妇及不足月非先兆早产原因住院孕妇共45例,其...目的检测先兆早产患者外周血促肾上腺皮质激素释放激素（CRH）浓度,探讨其浓度与先兆早产症状的相关性及影响其浓度的因素,以探讨CRH浓度作为预测早产指标的可行性。方法选取先兆早产住院孕妇及不足月非先兆早产原因住院孕妇共45例,其中先兆早产组（孕28~36周）22例,对照组（孕28~36周、无先兆早产症状的孕妇）23例。采每位受试对象抗凝外周血5 m L,分离血浆,采用ELISA法测定CRH浓度。结果先兆早产组孕妇外周血CRH浓度与对照组孕妇相比显著升高（P〈0.01）。先兆早产组与对照组的新生儿出生Apgar评分（1和5 min）均〉7分,且2组比较新生儿出生Apgar评分无统计学差异（P〉0.05）。先兆早产组与对照组比较,宫颈Bishop评分无统计学差异（P〉0.05）,先兆早产组中CRH浓度与宫颈Bishop评分无明显关系（P〉0.05）。先兆早产组与对照组中,双胎者与单胎者相比,胎膜早破者与非胎膜早破者相比,CRH浓度的差异均有统计学意义（均P〈0.05）。结论血浆CRH浓度明显升高是先兆早产的显著指标。血浆CRH浓度不影响新生儿出生状态和宫颈成熟度,但受妊娠胎儿数影响,且与胎膜早破密切相关。血浆CRH浓度有望成为预测先兆早产的指标,但其影响因素较多,应在分析其影响因素后进一步研究其预测定量值。展开更多
Restraint water-immersion stress(RWIS), a compound stress model, has been widely used to induce acute gastric ulceration in rats. A wealth of evidence suggests that the central nucleus of the amygdala(CEA) is a focal ...Restraint water-immersion stress(RWIS), a compound stress model, has been widely used to induce acute gastric ulceration in rats. A wealth of evidence suggests that the central nucleus of the amygdala(CEA) is a focal region for mediating the biological response to stress. Different stressors induce distinct alterations of neuronal activity in the CEA; however, few studies have reported the characteristics of CEA neuronal activity induced by RWIS. Therefore, we explored this issue using immunohistochemistry and in vivo extracellular single-unit recording. Our results showed that RWIS and restraint stress(RS) differentially changed the c-Fos expression and firing properties of neurons in the medial CEA. In addition,RWIS, but not RS, induced the activation of corticotropinreleasing hormone neurons in the CEA. These findings suggested that specific neuronal activation in the CEA is involved in the formation of RWIS-induced gastric ulcers.This study also provides a possible theoretical explanation for the different gastric dysfunctions induced by different stressors.展开更多
文摘目的:观察隔药饼灸对功能性消化不良(FD)肝郁脾虚模型大鼠HPA轴中枢相关受体及激素的影响,探讨隔药饼灸治疗FD可能的作用机制。方法:将SD大鼠随机分为空白(A)组、模型(B)组、隔药饼灸(C)组、艾炷灸(D)组和逍遥散(E)组,每组10只,除A组外,其余4组采用复合病因造模法造模,连续3周;再按被试因素施加方法,分别进行隔药饼灸、艾炷灸和逍遥散灌胃治疗14d;实验结束后采用RT-PCR检测下丘脑、垂体糖皮质激素受体(GR)m RNA表达,ELISA检测下丘脑促肾上腺皮质激素释放激素(CRH)、垂体促肾上腺皮质激素(ACTH)及肾上腺皮质酮(CORT)的含量。结果:与A组比较,B组大鼠GR m RNA在下丘脑及垂体的表达显著下降(P<0.01),且下丘脑CRH、垂体ACTH、肾上腺CORT含量显著上升(P<0.01);与B组比较,C、D、E组治疗组大鼠GR m RNA在下丘脑及垂体的表达显著上升(P<0.01),且下丘脑CRH、垂体ACTH、肾上腺CORT含量均显著下降(P<0.01);与E组比较,C组大鼠GR m RNA在下丘脑及垂体的表达及下丘脑CRH、垂体ACTH、肾上腺CORT含量相当;而D组大鼠GR m RNA在下丘脑及垂体的表达较C、E组较低(P<0.01),且下丘脑CRH、垂体ACTH、肾上腺CORT含量较C、E组较高(P<0.05)。结论:隔药饼灸能显著上调FD模型大鼠下丘脑、垂体GR m RNA表达,下调其下丘脑CRH、垂体ACTH、肾上腺CORT含量,其作用与逍遥散相当,优于艾炷灸。通过纠正HPA轴中枢相关受体及激素的异常表达,抑制HPA轴功能亢进,改善胃肠动力,这可能是隔药饼灸法治疗FD的另一作用机制。
文摘目的检测先兆早产患者外周血促肾上腺皮质激素释放激素（CRH）浓度,探讨其浓度与先兆早产症状的相关性及影响其浓度的因素,以探讨CRH浓度作为预测早产指标的可行性。方法选取先兆早产住院孕妇及不足月非先兆早产原因住院孕妇共45例,其中先兆早产组（孕28~36周）22例,对照组（孕28~36周、无先兆早产症状的孕妇）23例。采每位受试对象抗凝外周血5 m L,分离血浆,采用ELISA法测定CRH浓度。结果先兆早产组孕妇外周血CRH浓度与对照组孕妇相比显著升高（P〈0.01）。先兆早产组与对照组的新生儿出生Apgar评分（1和5 min）均〉7分,且2组比较新生儿出生Apgar评分无统计学差异（P〉0.05）。先兆早产组与对照组比较,宫颈Bishop评分无统计学差异（P〉0.05）,先兆早产组中CRH浓度与宫颈Bishop评分无明显关系（P〉0.05）。先兆早产组与对照组中,双胎者与单胎者相比,胎膜早破者与非胎膜早破者相比,CRH浓度的差异均有统计学意义（均P〈0.05）。结论血浆CRH浓度明显升高是先兆早产的显著指标。血浆CRH浓度不影响新生儿出生状态和宫颈成熟度,但受妊娠胎儿数影响,且与胎膜早破密切相关。血浆CRH浓度有望成为预测先兆早产的指标,但其影响因素较多,应在分析其影响因素后进一步研究其预测定量值。
基金supported by the National Natural Science Foundation of China (31571104 and 81501149)the Science and Technological Project of Shandong Province of China (2016GSF201058)
文摘Restraint water-immersion stress(RWIS), a compound stress model, has been widely used to induce acute gastric ulceration in rats. A wealth of evidence suggests that the central nucleus of the amygdala(CEA) is a focal region for mediating the biological response to stress. Different stressors induce distinct alterations of neuronal activity in the CEA; however, few studies have reported the characteristics of CEA neuronal activity induced by RWIS. Therefore, we explored this issue using immunohistochemistry and in vivo extracellular single-unit recording. Our results showed that RWIS and restraint stress(RS) differentially changed the c-Fos expression and firing properties of neurons in the medial CEA. In addition,RWIS, but not RS, induced the activation of corticotropinreleasing hormone neurons in the CEA. These findings suggested that specific neuronal activation in the CEA is involved in the formation of RWIS-induced gastric ulcers.This study also provides a possible theoretical explanation for the different gastric dysfunctions induced by different stressors.
