摘要
为证明下丘脑产生的促肾上腺皮质激素释放激素（ＣＲＨ）与白细胞介素－６（ＩＬ－６）和肿瘤坏死因子α（ＴＮＦα）的中枢作用有关，应用免疫组织化学方法，通过观察即早基因ｃ－ｆｏｓ的表达，对ＩＬ－６和ＴＮＦα经脑室引入后对下丘脑ＣＲＨ神经元的兴奋作用进行了研究。结果显示，在脑室注射ＩＬ－６或ＴＮＦα５ｈ后，下丘脑室旁核小细胞部出现强烈Ｆｏｓ表达。免疫组织化学双标记法显示，两种细胞因子诱导的Ｆｏｓ表达细胞中均有许多细胞同时呈现ＣＲＨ免疫反应。相比之下，双标细胞数量在ＩＬ－６引入者较ＴＮＦα引入者多。该结果表明。
In order to demonstrate the involvement of corticotropin releasing hormone (CRH) in the central effect of IL 6 and TNFα. The present study investigated immunohistochemically the Fos expression in hypothalamic CRH containing neurons following intracerebroventricular (Icv) administration of IL 6 and TNFα. A strong Fos expression was found in the parvocellular subdivision of paraventricular nucleus (PVN) 5 hours after IL 6 or TNFα injection. Fos and CRH double immunohistochemical staining revealed that a part of Fos expression neurons induced both by IL 6(Icv) or TNFα (Icv), belonged to CRH containing neuorns. More double labeled cells were found in the case of IL 6 injection. The present result indicated that the hypothalamic CRH containing neurons could be activated in different degree by centrally administration of IL 6 and TNFα.
出处
《解剖学报》
CAS
CSCD
北大核心
1997年第1期4-9,共6页
Acta Anatomica Sinica
关键词
白细胞介素6
肿瘤坏死因子Α
下丘脑
CRH
神经元
Interleukin 6
Tumor Necrosis Factor α
Corticotropin Releasing Hormone
Paraventricular Nucleus
Fos immunohistochemistry
rat
