The most frequent cause of death of patients with osteosarcoma is the metastasis of tumour cells. In spite of successful control of the primary tumour, the mortality of the patients due to metastatic spread is more th...The most frequent cause of death of patients with osteosarcoma is the metastasis of tumour cells. In spite of successful control of the primary tumour, the mortality of the patients due to metastatic spread is more than 30% within 5 years. 1 Recent studies about osteosarcoma metastatic mechanism are based on osteosarcoma matrilineal cell lines. 2 For further studies of metastatic mechanism of osteosarcoma the establishment of a better metastatic experimental model of osteosarcoma is needed. We isolated and established two cell sublines, with high and low metastatic potentials, respectively, derived from human osteosarcoma MG-63 cell line by cloning in vitro and transplantation in vivo , then analysed and identified their biological characteristics.展开更多
Using the modified HABM (Human amniotic basement membrane) model,an invasive subline(HR-8348-Ⅰ)was selected frorn the human rectal adenocarcinoma cell line (HR-8348).The invasiveness,motility and other biological cha...Using the modified HABM (Human amniotic basement membrane) model,an invasive subline(HR-8348-Ⅰ)was selected frorn the human rectal adenocarcinoma cell line (HR-8348).The invasiveness,motility and other biological characteristics of the selected cells and the parent cells were assayed comparatively. Although no significant differences(P>0. 05) were found in the mitotic index,growth curve,doubling time and plate efficiency,the invasiveness,migrating ability and attachment of the subline cells increased significantly(P<0.01).The subline was nearly triploid,with wider chromosome distribution,and was characterized by possessing more surface projections (filiform pseudopods),thicker ruthenium red-positive coat,than the parent cells , which were hypertriploid.The results suggest that HR-8348-Ⅰis a highly invasive variant isolated from the subpopulation of HR-8348,and could be useful in defining the biochemical and genetic events mediating invasion.展开更多
基金Theprojectwassupportedbythe973ProjectofChina (No 2002CB513107)
文摘The most frequent cause of death of patients with osteosarcoma is the metastasis of tumour cells. In spite of successful control of the primary tumour, the mortality of the patients due to metastatic spread is more than 30% within 5 years. 1 Recent studies about osteosarcoma metastatic mechanism are based on osteosarcoma matrilineal cell lines. 2 For further studies of metastatic mechanism of osteosarcoma the establishment of a better metastatic experimental model of osteosarcoma is needed. We isolated and established two cell sublines, with high and low metastatic potentials, respectively, derived from human osteosarcoma MG-63 cell line by cloning in vitro and transplantation in vivo , then analysed and identified their biological characteristics.
文摘Using the modified HABM (Human amniotic basement membrane) model,an invasive subline(HR-8348-Ⅰ)was selected frorn the human rectal adenocarcinoma cell line (HR-8348).The invasiveness,motility and other biological characteristics of the selected cells and the parent cells were assayed comparatively. Although no significant differences(P>0. 05) were found in the mitotic index,growth curve,doubling time and plate efficiency,the invasiveness,migrating ability and attachment of the subline cells increased significantly(P<0.01).The subline was nearly triploid,with wider chromosome distribution,and was characterized by possessing more surface projections (filiform pseudopods),thicker ruthenium red-positive coat,than the parent cells , which were hypertriploid.The results suggest that HR-8348-Ⅰis a highly invasive variant isolated from the subpopulation of HR-8348,and could be useful in defining the biochemical and genetic events mediating invasion.
文摘目的建立一种利用单碱基延伸终止结合荧光偏振技术(Fluorescence polarization,FP)快速检测B型流感By(B/Yamagata)和Bv(B/Victoria)亚系的新方法。方法从GenBank下载By和Bv血凝素(HA)基因各50条序列,通过MEGA软件进行分析,利用Primer Premier 5.0设计B型流感病毒亚系特异性引物和单标记延伸引物,建立单碱基延伸终止法与荧光偏振检测方法。结果 40株Bv和40株By病毒用单碱基延伸终止法与荧光偏振检测,与传统的基因测序结果一致性达100%。结论本方法操作简便、敏感性及特异性高、判读结果直观明确、检测费用低,适合于流感监测实验室对流感病毒的快速分子诊断。
