摘要
本文作者选用丁酸钠处理克隆化高转移人肺巨细胞癌（PGCL3）细胞，发现肿瘤细胞在形态、增殖速度、分裂指数、软琼脂集落形成能力和凝集反应等方面均出现向正常细胞表型逆转，证明丁酸钠具有抑制PGCL3细胞增殖及诱导分化的作用。我们还进行了反映肿瘤细胞浸润和转移能力的粘附、运动和降解侵袭实验，结果显示丁酸钠对PGCL3细胞的上述三种能力都有明显的抑制作用，表明丁酸钠在诱导PGCL3细胞向成熟方向分化的同时，还使PGCL3细胞的侵袭能力减弱。
Highly metastatic human lung cancer cell subline (PGCL3) cells were treated with sodium butyrate in vitro. We found that PGCL3 cells were reversed similar to the phenotypes of normal cell in morphology, proliferation rate, mitotic index,colony—forming ability in semisolid agar culture and Con A agglutination reaction. These results showed that sodium butyrate could inhibited proliferation and induced differentiation of PGCL3 cells.We also found that sodium butyrate treatment could result in significant inhibition of adhesion of cells to laminin substrate, migrative ability through the polycarbonate filter of Boyden chamber,and invasive ability through matrigel. Our studies identified that sodium butyrate could induced differentiation of PGCL3 cells,which was accompanied by decline in invasive ability.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1998年第2期111-114,共4页
Cancer Research on Prevention and Treatment
关键词
肺肿瘤
丁酸钠
诱导分化
侵袭能力
Sodium butyrate
Highly metastatic Human lung cancer cell subline
Induced differentiation:Invasive ability
