Sorting high-quality sperm with intact DNA,normal morphology,and active motility is crucial for clinically assisted reproductive technology,which influences the success of treatment and the health of offspring.Current...Sorting high-quality sperm with intact DNA,normal morphology,and active motility is crucial for clinically assisted reproductive technology,which influences the success of treatment and the health of offspring.Currently,microfluidic technology has been developed as a powerful platform for sperm sorting owing to its ability to manipulate fluid at the microscale and handle small samples.Specifically,microfluidic technology provides the necessary stimuli including fluid stimulus,chemical induction,and shape sift,which supports researchers in developing various sperm-sorting devices.According to the sorting principle,these devices can be divided into three categories:active sorting devices based on sperm rheological properties,passive sorting devices based on sperm physical properties,and external stimuli-induced sorting devices.Hence,we review a broad range of researches about sperm sorting with microfluidics and briefly present the properties of sperm and female reproductive tract to assist the design of microfluidic sperm sorting devices.展开更多
目的研究自制微流控芯片优选人类精子的效果。方法自行构建微流控芯片装置。收集男性不育患者的精液样本:(1)分别置于无毒灭菌离心管与微流控芯片中1h、2h、3h、4h,比较各时间点两组精子活力的变化;(2)分别用微流控芯片优选精子40min、6...目的研究自制微流控芯片优选人类精子的效果。方法自行构建微流控芯片装置。收集男性不育患者的精液样本:(1)分别置于无毒灭菌离心管与微流控芯片中1h、2h、3h、4h,比较各时间点两组精子活力的变化;(2)分别用微流控芯片优选精子40min、60min、80min,比较各时间点优选前后各项精子参数的变化;(3)分别用微流控芯片、上游法和密度梯度离心法优选精子,比较三种方法优选前后各项精子参数的变化。结果(1)静置1h、2h、3h、4h后,无毒灭菌离心管与微流控芯片中的精子活动率与前向运动精子百分率均无显著性差异(P>0.05)。(2)与优选前相比,微流控芯片优选精子40min、60min、80min后,精子活动率(36.54%vs 96.55%、97.92%、88.12%)和前向运动精子百分率(28.01%vs 83.04%、83.01%、81.25%)均显著上升(P<0.01),而精子浓度(47.11×10^(6)/m L vs 1.68×10^(6)/mL、3.81×10^(6)/mL、4.02×10^(6)/m L)均显著降低(P<0.01),但60min与80min组的精子浓度显著高于40min组(P<0.05);(3)与上游法和密度梯度离心法相比,微流控芯片优选后的精子活动率(89.58%vs 70.76%、71.89%)和前向运动精子百分率(82.35%vs 51.52%、45.99%)显著提升,而精子浓度(4.09×10^(6)/mL vs 17.06×10^(6)/mL、60.33×10^(6)/m L)和DFI(15.53%vs 30.08%、40.52%)显著降低(P<0.05)。结论微流控芯片对精子无毒并能优选出高质量精子,与两种常规精子优选方法相比,其优选后的精子DNA损伤较小,更适合于辅助生殖技术IVF/ICSI中使用。展开更多
对马鹿X/Y精子进行分离、冷冻保存和人工授精,探讨马鹿鲜精保存运输、精子分离以及性控冻精的受胎及后代性别控制准确率。结果表明:①马鹿鲜精中加入三联抗生素、18℃恒温保存最有利于精子活力的保持。②马鹿鲜精死精率与X/Y精子分离速...对马鹿X/Y精子进行分离、冷冻保存和人工授精,探讨马鹿鲜精保存运输、精子分离以及性控冻精的受胎及后代性别控制准确率。结果表明:①马鹿鲜精中加入三联抗生素、18℃恒温保存最有利于精子活力的保持。②马鹿鲜精死精率与X/Y精子分离速度有明显相关性,死精率越低,分离速度越快。③来自同一马鹿供体的3组样品分离精子解冻后活力(0.61 vs 0.60 vs 0.65)及4h时活力(0.30vs0.30vs0.32)无显著差异(P>0.05)。④应用马鹿性控冻精对80只发情母鹿进行子宫角深部输精,有55只母鹿妊娠期满产下仔鹿,情期受胎率67.5%,均为雄性,性别控制准确率100%。结论:分离马鹿X/Y精子的冷冻保存、人工授精,在技术上已达到产业化应用水平,通过进一步改善后,可以大规模推广应用。展开更多
基金This work was supported by the National Key Research and Development Program of China(2020YFA0908200)the National Natural Science Foundation of China(T2225003,52073060 and 61927805)+1 种基金Guangdong Basic and Applied Basic Research Foundation(2021B1515120054)the Shenzhen Fundamental Research Pro-gram(JCYJ20190813152616459 and JCYJ20210324133214038).
文摘Sorting high-quality sperm with intact DNA,normal morphology,and active motility is crucial for clinically assisted reproductive technology,which influences the success of treatment and the health of offspring.Currently,microfluidic technology has been developed as a powerful platform for sperm sorting owing to its ability to manipulate fluid at the microscale and handle small samples.Specifically,microfluidic technology provides the necessary stimuli including fluid stimulus,chemical induction,and shape sift,which supports researchers in developing various sperm-sorting devices.According to the sorting principle,these devices can be divided into three categories:active sorting devices based on sperm rheological properties,passive sorting devices based on sperm physical properties,and external stimuli-induced sorting devices.Hence,we review a broad range of researches about sperm sorting with microfluidics and briefly present the properties of sperm and female reproductive tract to assist the design of microfluidic sperm sorting devices.
文摘目的研究自制微流控芯片优选人类精子的效果。方法自行构建微流控芯片装置。收集男性不育患者的精液样本:(1)分别置于无毒灭菌离心管与微流控芯片中1h、2h、3h、4h,比较各时间点两组精子活力的变化;(2)分别用微流控芯片优选精子40min、60min、80min,比较各时间点优选前后各项精子参数的变化;(3)分别用微流控芯片、上游法和密度梯度离心法优选精子,比较三种方法优选前后各项精子参数的变化。结果(1)静置1h、2h、3h、4h后,无毒灭菌离心管与微流控芯片中的精子活动率与前向运动精子百分率均无显著性差异(P>0.05)。(2)与优选前相比,微流控芯片优选精子40min、60min、80min后,精子活动率(36.54%vs 96.55%、97.92%、88.12%)和前向运动精子百分率(28.01%vs 83.04%、83.01%、81.25%)均显著上升(P<0.01),而精子浓度(47.11×10^(6)/m L vs 1.68×10^(6)/mL、3.81×10^(6)/mL、4.02×10^(6)/m L)均显著降低(P<0.01),但60min与80min组的精子浓度显著高于40min组(P<0.05);(3)与上游法和密度梯度离心法相比,微流控芯片优选后的精子活动率(89.58%vs 70.76%、71.89%)和前向运动精子百分率(82.35%vs 51.52%、45.99%)显著提升,而精子浓度(4.09×10^(6)/mL vs 17.06×10^(6)/mL、60.33×10^(6)/m L)和DFI(15.53%vs 30.08%、40.52%)显著降低(P<0.05)。结论微流控芯片对精子无毒并能优选出高质量精子,与两种常规精子优选方法相比,其优选后的精子DNA损伤较小,更适合于辅助生殖技术IVF/ICSI中使用。
文摘对马鹿X/Y精子进行分离、冷冻保存和人工授精,探讨马鹿鲜精保存运输、精子分离以及性控冻精的受胎及后代性别控制准确率。结果表明:①马鹿鲜精中加入三联抗生素、18℃恒温保存最有利于精子活力的保持。②马鹿鲜精死精率与X/Y精子分离速度有明显相关性,死精率越低,分离速度越快。③来自同一马鹿供体的3组样品分离精子解冻后活力(0.61 vs 0.60 vs 0.65)及4h时活力(0.30vs0.30vs0.32)无显著差异(P>0.05)。④应用马鹿性控冻精对80只发情母鹿进行子宫角深部输精,有55只母鹿妊娠期满产下仔鹿,情期受胎率67.5%,均为雄性,性别控制准确率100%。结论:分离马鹿X/Y精子的冷冻保存、人工授精,在技术上已达到产业化应用水平,通过进一步改善后,可以大规模推广应用。
