摘要
具有多维网络微通道结构的微流控芯片可在微纳尺度上集成细胞进样、培养、分选、裂解和分离检测等多种功能单元,不仅在尺寸上与精细胞匹配,还可为精细胞提供相对封闭的接近生理状态的生长微环境。研究者已利用此系统的层流、微通道特殊几何结构等特点对精子进行了多方面研究。该文对微流控芯片技术在精细胞的培养、分选、胞内成分分析和人工授精中的应用进行了综述,介绍了用于精细胞研究的多种微流控芯片系统,并讨论了精细胞分选的各种方法。
With the sophistication and variety of microchannels, microfluidic chip which is integrated with muhifunctional units including cell loading, culturing, sorting, lysis, separation and detec- tion, not only matches sperm cells in size, but also supplies them with relatively closed growing mi- croenvironments close to physiological level. Some investigations on sperm based on laminar flow and special microchannel geometry were studied. In this paper, the applications of microfluidic chip sys- tems in sperm cell culturing, sorting, intracellular contents analysis and in-vitro fertilization （IVF） were reviewed. Several microfluidic chip systems used to study sperm cell were introduced. Varied sorting methods on sperm cell were also discussed.
出处
《分析测试学报》
CAS
CSCD
北大核心
2014年第4期483-488,共6页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(21305034)
河南科技大学校青年基金资助项目(2012QN014)
关键词
微流控芯片
精细胞
培养
分选
成分分析
人工授精
microfluidic chip
sperm cell
culturing
sorting
intracellular contents analysis
in-vitro fertilization （IVF）
