Background:The goal was to quantitatively analyze the bulbar conjunctival microvascular density using optical coherence tomography angiography(OCTA)and compare it to the vessel density using functional slit-lamp biomi...Background:The goal was to quantitatively analyze the bulbar conjunctival microvascular density using optical coherence tomography angiography(OCTA)and compare it to the vessel density using functional slit-lamp biomicroscopy(FSLB).Methods:Temporal bulbar conjunctiva of 20 eyes(10 healthy subjects)was imaged using both OCTA and FSLB.Image processing was performed including equalization,de-noising,thresholding,and skeletonization.The vessel density was measured by fractal analysis(box counting,Dbox)and pixel counting(%).Results:Vessel density(Dbox)of the bulbar conjunctiva obtained using OCTA was 1.28±0.01 Dbox,which was significantly lower than the result(1.32±0.01 Dbox,P<0.001)obtained using FSLB.Furthermore,the vessel density(%)obtained using OCTA was 3.31±0.12%,which was also significantly lower than the result(3.69±0.16%,P<0.001)obtained using FSLB.No significant correlations(r ranged from 0.21 to 0.32,P>0.05)between both instruments were found in both vessel density methods(Dbox and percentage).However,in each of the devices,vessel density in Dbox was significantly correlated with the vessel density in percentage(r=1.0 for FSLB and r=0.98 for OCTA,both P<0.001).Conclusion:This study demonstrated that the vessel density of the bulbar conjunctiva obtained using OCTA can be quantified,and the results were not compatible with that obtained using slit-lamp biomicroscopy photography.展开更多
目的:探讨在Van Herick法基础上更方便准确评估前房角宽度的方法。方法:纳入2021-01/12于我院就诊的年龄相关性白内障患者58例69眼,参考Van Herick法分为房角宽度≥1/2颞侧角膜厚度(CT)组(37例44眼)和<1/2CT组(21例25眼),应用超声生...目的:探讨在Van Herick法基础上更方便准确评估前房角宽度的方法。方法:纳入2021-01/12于我院就诊的年龄相关性白内障患者58例69眼,参考Van Herick法分为房角宽度≥1/2颞侧角膜厚度(CT)组(37例44眼)和<1/2CT组(21例25眼),应用超声生物显微镜测量中央前房深度和周边房角度数。结果:房角宽度≥1/2CT组和<1/2CT组患者中央前房深度有明显差异(2.64±0.27 mm vs 2.23±0.29 mm,P<0.01),且两组间上方、颞侧、下方和鼻侧象限房角度数均有明显差异(P<0.01)。房角宽度≥1/2CT组患者上方与下方象限房角度数无显著差异(P>0.05),其余各象限房角度数均有差异(P<0.05);房角宽度<1/2CT组患者上方与鼻、颞侧象限,下方与颞侧象限房角度数均有差异(P<0.05)。结论:裂隙灯下采用Van Herick法评估颞侧房角宽度,同时评估下方象限房角宽度,可以更简单、快速、准确地评估前房角的整体情况。展开更多
AIM:To compare the results of non-mydriatic ultra-wide field imaging system,mydriatic slit-lamp lens(Volk+90 D) and mydriatic Goldmann three-mirror contact lens examinations in screening fundus lesions among patie...AIM:To compare the results of non-mydriatic ultra-wide field imaging system,mydriatic slit-lamp lens(Volk+90 D) and mydriatic Goldmann three-mirror contact lens examinations in screening fundus lesions among patients after cataract surgery.METHODS:Non-mydriatic images were obtained with an Optomap panoramic 200Tx(Optomap 200Tx) 3d after surgery and graded by a blinded ophthalmologist.A mydriatic slit-lamp lens examination was performed by another blinded retinal specialist on the same day.A third blinded retinal specialist examined patients two weeks after surgery using a Goldmann three-mirror contact lens.RESULTS:In total,160 patients(184 eyes) were examined,and 66,69,and 75 cases of retinal lesion(s)were identified using the Optomap 200 Tx,slit-lamp lens,and Goldmann three-mirror contact lens,respectively.In13 cases,fundus changes were sight-threatening.The results obtained by Optomap 200 Tx examination and by mydriatic slit-lamp lens examination have good consistency(P =0.375,Kappa =0.942).The mydriatic Goldmann three-mirror lens examination revealed more fundus lesions but are consistent with Optomap 200Tx(P=0.004,Kappa =0.897) and mydriatic slit-lamp lens examination(P=0.031,Kappa=0.932).CONCLUSION:Early post-operative fundus screening in cataract patients is extremely important and necessary to prevent further vision loss.Wide-field imaging is a feasible and convenient tool for fundus examination that can be used as a primary screening method among patients after cataract surgery.展开更多
Background:The inter-visit variation of measuring bulbar conjunctival microvasculature and microcirculation needs to be considered when the results from multiple visits are interpreted.This study examined the inter-vi...Background:The inter-visit variation of measuring bulbar conjunctival microvasculature and microcirculation needs to be considered when the results from multiple visits are interpreted.This study examined the inter-visit variability of measuring conjunctival microvasculature and microcirculation in habitual contact lens(HCL)wearers and non-contact lens(NCL)wearers.Methods:Twenty-eight subjects were recruited including 13 HCL wearers(10 females and 3 males;mean age±standard deviation,25.8±4.6 years)who had worn contact lenses on a daily basis for at least 3 years and 15 NCL wearers(10 females and 5 males,age 25.5±4.0 years)were recruited.The temporal bulbar conjunctiva was imaged using a functional slit-lamp bio-microscope(FSLB)imaging system.FSLB imaging was performed in the morning when the HCL wearers did not wear their lenses.The measurements included conjunctival vessel diameter,vessel density,blood flow velocity and flow volume.In addition,conjunctival microvasculature was analyzed using monofractal(Dbox,representing vessel density)and multifractal(D0 representing vessel complexity)analyses.The repeated measurement was conducted at least one week after the first visit and both eyes of each participant were imaged.The coefficient of variation(CV)was calculated as the standard deviation of the differences between test and re-test then divided by the mean of the measurements.The intra-class correlation coefficient(ICC)was also calculated.Results:No significant differences of all vascular measurements in both the right and left eyes were found between two groups(P>0.05).Between two measurements on two different visits,the CV was from 2.4%(vessel density D0)to 63.5%(blood flow volume Q)in HCL wearers and from 3.4%(D0)to 40.6%(blood flow volume)in NCL wearers.The ICC was from 0.60(vessel diameter)to 0.81(axial blood flow velocity VA)in HCL wearers and from 0.44(Q)to 0.68(cross-sectional blood flow velocity VS)in NCL wearers.Conclusions:The measurement variability of the vessel density of the bulbar conjunctiva 展开更多
基金supported by NIH Center Grant P30 EY014801grant from the Research to Prevent Blindness(RPB)+1 种基金supported by Guangzhou Science and Technology Project(No.201804010038)“Yangcheng Scholar”Youth Research Backbone Training Project of Guangzhou Municipal College(No.1201581612).
文摘Background:The goal was to quantitatively analyze the bulbar conjunctival microvascular density using optical coherence tomography angiography(OCTA)and compare it to the vessel density using functional slit-lamp biomicroscopy(FSLB).Methods:Temporal bulbar conjunctiva of 20 eyes(10 healthy subjects)was imaged using both OCTA and FSLB.Image processing was performed including equalization,de-noising,thresholding,and skeletonization.The vessel density was measured by fractal analysis(box counting,Dbox)and pixel counting(%).Results:Vessel density(Dbox)of the bulbar conjunctiva obtained using OCTA was 1.28±0.01 Dbox,which was significantly lower than the result(1.32±0.01 Dbox,P<0.001)obtained using FSLB.Furthermore,the vessel density(%)obtained using OCTA was 3.31±0.12%,which was also significantly lower than the result(3.69±0.16%,P<0.001)obtained using FSLB.No significant correlations(r ranged from 0.21 to 0.32,P>0.05)between both instruments were found in both vessel density methods(Dbox and percentage).However,in each of the devices,vessel density in Dbox was significantly correlated with the vessel density in percentage(r=1.0 for FSLB and r=0.98 for OCTA,both P<0.001).Conclusion:This study demonstrated that the vessel density of the bulbar conjunctiva obtained using OCTA can be quantified,and the results were not compatible with that obtained using slit-lamp biomicroscopy photography.
文摘目的:探讨在Van Herick法基础上更方便准确评估前房角宽度的方法。方法:纳入2021-01/12于我院就诊的年龄相关性白内障患者58例69眼,参考Van Herick法分为房角宽度≥1/2颞侧角膜厚度(CT)组(37例44眼)和<1/2CT组(21例25眼),应用超声生物显微镜测量中央前房深度和周边房角度数。结果:房角宽度≥1/2CT组和<1/2CT组患者中央前房深度有明显差异(2.64±0.27 mm vs 2.23±0.29 mm,P<0.01),且两组间上方、颞侧、下方和鼻侧象限房角度数均有明显差异(P<0.01)。房角宽度≥1/2CT组患者上方与下方象限房角度数无显著差异(P>0.05),其余各象限房角度数均有差异(P<0.05);房角宽度<1/2CT组患者上方与鼻、颞侧象限,下方与颞侧象限房角度数均有差异(P<0.05)。结论:裂隙灯下采用Van Herick法评估颞侧房角宽度,同时评估下方象限房角宽度,可以更简单、快速、准确地评估前房角的整体情况。
基金Supported by the National Natural Science Foundation of China(No.81271045No.81470642)Shanghai Science and Technology Committee(No.15XD1502800)
文摘AIM:To compare the results of non-mydriatic ultra-wide field imaging system,mydriatic slit-lamp lens(Volk+90 D) and mydriatic Goldmann three-mirror contact lens examinations in screening fundus lesions among patients after cataract surgery.METHODS:Non-mydriatic images were obtained with an Optomap panoramic 200Tx(Optomap 200Tx) 3d after surgery and graded by a blinded ophthalmologist.A mydriatic slit-lamp lens examination was performed by another blinded retinal specialist on the same day.A third blinded retinal specialist examined patients two weeks after surgery using a Goldmann three-mirror contact lens.RESULTS:In total,160 patients(184 eyes) were examined,and 66,69,and 75 cases of retinal lesion(s)were identified using the Optomap 200 Tx,slit-lamp lens,and Goldmann three-mirror contact lens,respectively.In13 cases,fundus changes were sight-threatening.The results obtained by Optomap 200 Tx examination and by mydriatic slit-lamp lens examination have good consistency(P =0.375,Kappa =0.942).The mydriatic Goldmann three-mirror lens examination revealed more fundus lesions but are consistent with Optomap 200Tx(P=0.004,Kappa =0.897) and mydriatic slit-lamp lens examination(P=0.031,Kappa=0.932).CONCLUSION:Early post-operative fundus screening in cataract patients is extremely important and necessary to prevent further vision loss.Wide-field imaging is a feasible and convenient tool for fundus examination that can be used as a primary screening method among patients after cataract surgery.
基金This work was supported by a research grant provided by Johnson&Johnson Vision Care,Inc.
文摘Background:The inter-visit variation of measuring bulbar conjunctival microvasculature and microcirculation needs to be considered when the results from multiple visits are interpreted.This study examined the inter-visit variability of measuring conjunctival microvasculature and microcirculation in habitual contact lens(HCL)wearers and non-contact lens(NCL)wearers.Methods:Twenty-eight subjects were recruited including 13 HCL wearers(10 females and 3 males;mean age±standard deviation,25.8±4.6 years)who had worn contact lenses on a daily basis for at least 3 years and 15 NCL wearers(10 females and 5 males,age 25.5±4.0 years)were recruited.The temporal bulbar conjunctiva was imaged using a functional slit-lamp bio-microscope(FSLB)imaging system.FSLB imaging was performed in the morning when the HCL wearers did not wear their lenses.The measurements included conjunctival vessel diameter,vessel density,blood flow velocity and flow volume.In addition,conjunctival microvasculature was analyzed using monofractal(Dbox,representing vessel density)and multifractal(D0 representing vessel complexity)analyses.The repeated measurement was conducted at least one week after the first visit and both eyes of each participant were imaged.The coefficient of variation(CV)was calculated as the standard deviation of the differences between test and re-test then divided by the mean of the measurements.The intra-class correlation coefficient(ICC)was also calculated.Results:No significant differences of all vascular measurements in both the right and left eyes were found between two groups(P>0.05).Between two measurements on two different visits,the CV was from 2.4%(vessel density D0)to 63.5%(blood flow volume Q)in HCL wearers and from 3.4%(D0)to 40.6%(blood flow volume)in NCL wearers.The ICC was from 0.60(vessel diameter)to 0.81(axial blood flow velocity VA)in HCL wearers and from 0.44(Q)to 0.68(cross-sectional blood flow velocity VS)in NCL wearers.Conclusions:The measurement variability of the vessel density of the bulbar conjunctiva