目的 检测 SP在单眼剥夺弱视猫 L GN中的表达 ,并探讨其在弱视发病中的意义。方法 采用免疫组化 APA法 ,对 7例正常猫和 8例单眼剥夺弱视猫 L GN SP免疫阳性神经元及神经纤维进行定位并定量研究其变化。结果 在单眼剥夺猫剥夺侧 L GN...目的 检测 SP在单眼剥夺弱视猫 L GN中的表达 ,并探讨其在弱视发病中的意义。方法 采用免疫组化 APA法 ,对 7例正常猫和 8例单眼剥夺弱视猫 L GN SP免疫阳性神经元及神经纤维进行定位并定量研究其变化。结果 在单眼剥夺猫剥夺侧 L GN的A1层 SP表达显著下降 (P <0 .0 5 )。结论 单眼剥夺可造成剥夺侧 L GN剥夺眼相应输入层次的 SP表达下降 。展开更多
Purpose: To assess the effects of monocular lid closure during critical period on cortical activity.Method: Pattern visual evoked potentials (PVEP) of the normal and the monocular deprivation (MD) cats were dynamicall...Purpose: To assess the effects of monocular lid closure during critical period on cortical activity.Method: Pattern visual evoked potentials (PVEP) of the normal and the monocular deprivation (MD) cats were dynamically measured and the number of gam-maaminobutyric acid immunopositive (GABA-IP) neurones of the area 17 of the visual cortex and the lateral geniculate nucleus (LGN) was quantitatively compared by using immunohistochemical method (ABC).Result: The amplitude of the N1-P1 attenuated in deprived eyes (DE) , NE/DE at postnatal week (PNW) 7-8 (P【0. 05), NE/DE at PNW 15-16 (P【0. 01); while P1 latency delayed, NE/DE at PNW 7-8 (P】0. 05), NE/DE at PNW 15 - 16(P【0. 05). The numbers of GABA-IP neurones in layer A1 of the ipsilateral LGN and in layer A of the contralateral LGN, compared to those in the corresponding normal laminae, were not significant at PNW 7 - 8 and PNW 11 - 12 (P 】0. 05), while in the same cats a reduction in the number of GABA-IP neurcnes was found in layer IV of area 17 at PNW展开更多
AIM: Many studies have demonstrated N-methyl-D-aspartate receptor-1-subunit (NMDAR1) is associated with amblyopia. The effectiveness of levodopa in improving the visual function of the children with amblyopia has also...AIM: Many studies have demonstrated N-methyl-D-aspartate receptor-1-subunit (NMDAR1) is associated with amblyopia. The effectiveness of levodopa in improving the visual function of the children with amblyopia has also been proved. But the mechanism is undefined. Our study was to explore the possible mechanism. METHODS: Sixty 14-day-old healthy SD rats were randomly divided into 4 groups, including normal group, monocular deprivation group, levodopa group and normal saline group, 15 rats each. We sutured all the rats' unilateral eyelids except normal group to establish the monocular deprivation animal model and raise them in normal sunlight till 45-day-old. NMDAR1 was detected in the visual cortex with immunohistochemistry methods, Western Blot and Real time PCR. LD and NS groups were gavaged with levodopa (40mg/kg) and normal saline for 28 days respectively. NMDAR1 was also detected with the methods above. RESULTS: NMDAR1 in the visual cortex of MD group was less than that of normal group. NMDAR1 in the visual cortex of LD group was more than that of NS group. CONCLUSION: NMDAR1 is associated with the plasticity of visual development. Levodopa may influence the expression of NMDAR1 and improve visual function, and its target may lie in the visual cortex.展开更多
文摘Purpose: To assess the effects of monocular lid closure during critical period on cortical activity.Method: Pattern visual evoked potentials (PVEP) of the normal and the monocular deprivation (MD) cats were dynamically measured and the number of gam-maaminobutyric acid immunopositive (GABA-IP) neurones of the area 17 of the visual cortex and the lateral geniculate nucleus (LGN) was quantitatively compared by using immunohistochemical method (ABC).Result: The amplitude of the N1-P1 attenuated in deprived eyes (DE) , NE/DE at postnatal week (PNW) 7-8 (P【0. 05), NE/DE at PNW 15-16 (P【0. 01); while P1 latency delayed, NE/DE at PNW 7-8 (P】0. 05), NE/DE at PNW 15 - 16(P【0. 05). The numbers of GABA-IP neurones in layer A1 of the ipsilateral LGN and in layer A of the contralateral LGN, compared to those in the corresponding normal laminae, were not significant at PNW 7 - 8 and PNW 11 - 12 (P 】0. 05), while in the same cats a reduction in the number of GABA-IP neurcnes was found in layer IV of area 17 at PNW
文摘AIM: Many studies have demonstrated N-methyl-D-aspartate receptor-1-subunit (NMDAR1) is associated with amblyopia. The effectiveness of levodopa in improving the visual function of the children with amblyopia has also been proved. But the mechanism is undefined. Our study was to explore the possible mechanism. METHODS: Sixty 14-day-old healthy SD rats were randomly divided into 4 groups, including normal group, monocular deprivation group, levodopa group and normal saline group, 15 rats each. We sutured all the rats' unilateral eyelids except normal group to establish the monocular deprivation animal model and raise them in normal sunlight till 45-day-old. NMDAR1 was detected in the visual cortex with immunohistochemistry methods, Western Blot and Real time PCR. LD and NS groups were gavaged with levodopa (40mg/kg) and normal saline for 28 days respectively. NMDAR1 was also detected with the methods above. RESULTS: NMDAR1 in the visual cortex of MD group was less than that of normal group. NMDAR1 in the visual cortex of LD group was more than that of NS group. CONCLUSION: NMDAR1 is associated with the plasticity of visual development. Levodopa may influence the expression of NMDAR1 and improve visual function, and its target may lie in the visual cortex.
