摘要
目的 探讨电针对单眼剥夺大鼠外侧膝状体Nogo-A和NgR蛋白表达的影响。方法 从40只SPF级新生SD大鼠中随机选取28只,于出生14d行右眼睑缝合方法建立单眼剥夺模型,其中造模成功的24只随机分为剥夺组和电针组。电针组在左、右两侧“攒竹”、“风池”实施电针治疗。P-VEP检测各组大鼠P-VEP的P100波振幅及潜时值差异;HE染色观察LGN中神经元的病理学形态改变;免疫组化和Western blot方法检测LGN中Nogo-A和NgR蛋白的表达水平。结果 与正常组比较,剥夺组细胞固缩;缝合眼P100波振幅显著降低、潜时值显著升高、LGN中的Nogo-A和NgR平均阳性细胞数增多、平均灰度值降低、相对表达量增加(P<0.01)。电针组与剥夺组比较,细胞形态明显改善;缝合眼P100波振幅显著升高(P<0.05),潜时值显著降低(P<0.01);LGN中Nogo-A和NgR蛋白的平均阳性细胞数减少、平均灰度值增加(P<0.05),相对表达量降低(P<0.01)。结论 电针可改善单眼剥夺后LGN中损伤的神经元细胞形态,其机制可能与抑制Nogo-A及NgR蛋白的过表达相关。
Objective To investigate the effect of electroacupuncture on the expression of Nogo-A and NgR proteins in the lateral geniculate body of monocular deprivation amblyopia rats.Methods Twenty-eight out of 40 SPF-grade newborn SD rats were randomly selected to establish a monocular deprivation rat model by right eyelid suture at 14d of birth,and 24 rats with successful modelling were randomly divided into the model group and the electroacupuncture group.In the electroacupuncture group,electroacupuncture was applied to the left and right sides of the eye at"Cuanzhu"and"Fengchi".Differences in the amplitude and latency values of the P100 wav eof the P-VEP of the rats in each group were detected by P-VEP;HE staining was used to observe the pathological morphological changes of neurons in LGN;immunohistochemistry and Western blot were used to detect the expression levels of Nogo-A and NgR proteins in the lateral geniculate body.Results Compared with the normal group,the cells of the deprived eye were solidified;the P100 wave amplitude in the model group was significantly decreased and the latency value was significantly increased,the mean cell number of Nogo-A and NgR proteins in LGN was increased,the average gray value was decreased,and the relative expression was increased(P<0.01).In the electroacupuncture group,compared with the model group,the cellular of the deprived eye morphology had a significantly improved;the P100 wave amplitude was significantly higher(P<0.05),and the latency value was significantly reduced(P<0.01);and the mean number of positive cells in LGN for Nogo-A and NgR proteins was reduced,with an increase in the mean gray value(P<0.05)and a decrease in the relative expression(P<0.01).Conclusion Electroacupuncture protects nerve cells damaged by monocular deprivation of the posterior LGN,and its mechanism of action may be associated with inhibition of the over expression of Nogo-A and NgR.
作者
胡晨莹
余博妍
李庆红
纪阳斌
吴锋
丁见
HU Chen-ying;YU Bo-yan;LI Qing-hong;JI Yang-bin;WU Feng;DING Jian(Department of Human Anatomy,School of Basic Medical Sciences,Wannan Medical College,Wuhu,Anhui,241002,China;School of Anesthesiology,Wannan Medical College)
出处
《承德医学院学报》
2024年第4期276-280,共5页
Journal of Chengde Medical University
基金
安徽省高校自然科学重点项目(2022AH051230)
安徽省中青年教师培养行动青年骨干教师境内访学研修资助项(JNFX2023032)
安徽省大学生创新训练计划项目(S202310368026)。
