AIM： To verify the expression and methylation status of the MAGE-A1 and MAGE-A3 genes in colorectal cancer tissues and cancer cell lines. METHODS： We evaluated promoter demethylation status of the MAGE-A1 and MAGE-A...AIM： To verify the expression and methylation status of the MAGE-A1 and MAGE-A3 genes in colorectal cancer tissues and cancer cell lines. METHODS： We evaluated promoter demethylation status of the MAGE-A1 and MAGE-A3 genes by RT-PCR analysis and methylation-specific PCR （MS-PCR）, as well as sequencing analysis, after sodium bisulfite modification in 32 colorectal cancer cell lines and 87 cancer tissues. RESULTS： Of the 32 cell lines, MAGE-A1 and MAGE-A3 expressions were observed in 59% and 66%, respectively. Subsequent to sodium bisulfite modification and MSPCR analysis, the promoter hypomethylation of MAGE-A1 and MAGE-A3 was confirmed in both at 81% each. Promoter hypomethylation of MAGE-A1 and MAGE-A3 in colorectal cancer tissues was observed in 43% and 77%, respectively. Hypomethylation of MAGE-A1 and MAGE-A3 genes in corresponding normal tissues were observed in 2% and 6%, respectively. CONCLUSION： The promoter hypomethylation of MAGE genes up-regulates its expression in colorectal carcinomas as well as in gastric cancers and might play a significant role in the development and progression of human colorectal carcinomas.展开更多
背景与目的apr1是我们在凋亡的人早幼粒白血病细胞HL-60中克隆的一个人类新基因,初步研究表明它可能是一个凋亡相关基因(GenBank ID NM_014061)。为进一步了解该基因的功能背景,我们克隆了该基因并进行了生物信息学分析和亚细胞定位。...背景与目的apr1是我们在凋亡的人早幼粒白血病细胞HL-60中克隆的一个人类新基因,初步研究表明它可能是一个凋亡相关基因(GenBank ID NM_014061)。为进一步了解该基因的功能背景,我们克隆了该基因并进行了生物信息学分析和亚细胞定位。方法利用逆转录PCR方法克隆该基因的cDNA序列,测序后利用生物信息学方法进行了基因组定位、氨基酸保守结构域搜索、同源蛋白比较和基因进化树分析;并进行了该基因的亚细胞定位。结果apr.1定位于人染色体上的Xp11.22,有两个MAGE家族结构域,基因进化上同源于MAGE.D1和Necdin,基因表达产物定位于真核细胞的细胞核。结论apr.1属于MAGE家族的一个新分子,可能属于Ⅱ类MAGE基因。展开更多
基金the Korea Research Foundation Grant, No.KRF-2003-03-E00199
文摘AIM： To verify the expression and methylation status of the MAGE-A1 and MAGE-A3 genes in colorectal cancer tissues and cancer cell lines. METHODS： We evaluated promoter demethylation status of the MAGE-A1 and MAGE-A3 genes by RT-PCR analysis and methylation-specific PCR （MS-PCR）, as well as sequencing analysis, after sodium bisulfite modification in 32 colorectal cancer cell lines and 87 cancer tissues. RESULTS： Of the 32 cell lines, MAGE-A1 and MAGE-A3 expressions were observed in 59% and 66%, respectively. Subsequent to sodium bisulfite modification and MSPCR analysis, the promoter hypomethylation of MAGE-A1 and MAGE-A3 was confirmed in both at 81% each. Promoter hypomethylation of MAGE-A1 and MAGE-A3 in colorectal cancer tissues was observed in 43% and 77%, respectively. Hypomethylation of MAGE-A1 and MAGE-A3 genes in corresponding normal tissues were observed in 2% and 6%, respectively. CONCLUSION： The promoter hypomethylation of MAGE genes up-regulates its expression in colorectal carcinomas as well as in gastric cancers and might play a significant role in the development and progression of human colorectal carcinomas.
文摘背景与目的apr1是我们在凋亡的人早幼粒白血病细胞HL-60中克隆的一个人类新基因,初步研究表明它可能是一个凋亡相关基因(GenBank ID NM_014061)。为进一步了解该基因的功能背景,我们克隆了该基因并进行了生物信息学分析和亚细胞定位。方法利用逆转录PCR方法克隆该基因的cDNA序列,测序后利用生物信息学方法进行了基因组定位、氨基酸保守结构域搜索、同源蛋白比较和基因进化树分析;并进行了该基因的亚细胞定位。结果apr.1定位于人染色体上的Xp11.22,有两个MAGE家族结构域,基因进化上同源于MAGE.D1和Necdin,基因表达产物定位于真核细胞的细胞核。结论apr.1属于MAGE家族的一个新分子,可能属于Ⅱ类MAGE基因。
