Objective:To evaluate the expression levels and correlations among the expressions of transforming growth factor-β1（TGF-β1）,Kunx3 and CD83 in colonic mucosal specimens from IBS patients.Methods:A total of 40 patie...Objective:To evaluate the expression levels and correlations among the expressions of transforming growth factor-β1（TGF-β1）,Kunx3 and CD83 in colonic mucosal specimens from IBS patients.Methods:A total of 40 patients were selected,who were confirmed as IBS by Rome III standard and 40 healthy volunteers served as control.Colonic mucosal specimens of each subject were collected from colon sigmoideum with biopsy forceps.Runx3,TGF-β1?and CD83(the marker for immunecompetent mature dendritic cells（DCs）mRNA in the sigmoid colon tissue were measured by real-time fluorescence quantitative PCR.Results:Compared with the control group,CD83 mRNA expressions were higher in patients with IBS than in healthy controls（P【0.05）and were associated with runt-related transcription factor 3（Runx3）mRNA levels（r=-0.361,P【0.05）.Meanwhile,Runx3 mRNA levels were associated with TGF-β1,mRNA expressions in irritable bowel syndrome（IBS）patients（r=0.402,P【0.05）.However,there was no correlation between the mRNA expressions of TGF-β1 and CD83（P】0.05）.Conclusions:The increase of abnormal dendritic cells might influence the occurrence and development of IBS.TGF-β1 signal pathway might not be involved in Runx 3-regulated maturation of dendritic cells in IBS.展开更多
基金supported by Key Science and Technology Project of Hainan Province(Grant No.ZDXM20100042)
文摘Objective:To evaluate the expression levels and correlations among the expressions of transforming growth factor-β1（TGF-β1）,Kunx3 and CD83 in colonic mucosal specimens from IBS patients.Methods:A total of 40 patients were selected,who were confirmed as IBS by Rome III standard and 40 healthy volunteers served as control.Colonic mucosal specimens of each subject were collected from colon sigmoideum with biopsy forceps.Runx3,TGF-β1?and CD83(the marker for immunecompetent mature dendritic cells（DCs）mRNA in the sigmoid colon tissue were measured by real-time fluorescence quantitative PCR.Results:Compared with the control group,CD83 mRNA expressions were higher in patients with IBS than in healthy controls（P【0.05）and were associated with runt-related transcription factor 3（Runx3）mRNA levels（r=-0.361,P【0.05）.Meanwhile,Runx3 mRNA levels were associated with TGF-β1,mRNA expressions in irritable bowel syndrome（IBS）patients（r=0.402,P【0.05）.However,there was no correlation between the mRNA expressions of TGF-β1 and CD83（P】0.05）.Conclusions:The increase of abnormal dendritic cells might influence the occurrence and development of IBS.TGF-β1 signal pathway might not be involved in Runx 3-regulated maturation of dendritic cells in IBS.
