Extranodal natural killer/T-cell lymphoma(ENKTL) is a distinct subtype of non-Hodgkin's lymphoma and is rare in the colon.Synchronous adenocarcinoma and ENKTL of the colon has not been reported in the literature.I...Extranodal natural killer/T-cell lymphoma(ENKTL) is a distinct subtype of non-Hodgkin's lymphoma and is rare in the colon.Synchronous adenocarcinoma and ENKTL of the colon has not been reported in the literature.In the present study,we report a 63-year-old male who suffered from intermittent bloody stools for 2 mo.He did not have fever,body weight loss or night sweat.Endoscopic and imaging studies revealed a 4.5-cm ulcerative mass in the ascending colon and a 3.0-cm polypoid,easy bleeding mass in the sigmoid colon,respectively.Thought to have double carcinoma of the colon,he received simultaneous right hemicolectomy and sigmoidectomy.The pathological diagnosis was a synchronous ENKTL(ascending colon) and adenocarcinoma(sigmoid colon).The literature on synchronous adenocarcinoma and malignant lymphoma of the colon was also reviewed.展开更多
目的:通过观察正常结肠组织及各类型结肠腺瘤息肉和结肠癌组织类型中瞬时受体阳离子通道亚家族V成员5(transient receptor potential cation channel,subfamily V,member5,TRPV5)、TRPV6表达情况,研究TRPV5、TRPV6的表达变化是否与结肠...目的:通过观察正常结肠组织及各类型结肠腺瘤息肉和结肠癌组织类型中瞬时受体阳离子通道亚家族V成员5(transient receptor potential cation channel,subfamily V,member5,TRPV5)、TRPV6表达情况,研究TRPV5、TRPV6的表达变化是否与结肠腺瘤-腺癌的演变过程相关,探讨其在人类结肠腺瘤-腺癌形成过程中的作用.方法:取肠镜活检或手术切除的增生性息肉、结肠腺瘤(免疫组织化学40例)、结肠癌标本各20例及正常对照肠黏膜20例.采用实时荧光定量PCR技术、Western blot技术及免疫组织化学技术检测并比较正常结肠组织、结肠腺瘤及结肠癌组织中TRPV5、TRPV6m R N A及其相应的蛋白含量的表达,同时通过免疫组织化学测定组织细胞Ki-67来观察TRPV5、TRPV6表达与上述组织细胞增殖的关系.结果:(1)TRPV5、TRPV6在正常结肠组织、结肠息肉组织及结肠癌组织中均有表达,且主要表达部位均在细胞胞浆;Ki-67表达在TRPV5或TRPV6表达增高时亦增高(P<0.05);(2)T R P V5、T R P V6的蛋白及m R N A在正常肠黏膜及非腺瘤息肉中呈弱阳性表达或不表达(P>0.05),在结肠腺瘤及结肠腺癌中高表达(P<0.05),且与组织类型具有直线相关性(P<0.05);(3)在结肠黏膜组织内,TRPV5表达量低于TRPV6(P<0.05).结论:在结肠黏膜组织内,TRPV5、TRPV6均有不同程度表达,特别在结肠腺瘤及结肠腺癌中呈高表达状态,同时Ki-67表达亦相应增高.提示在结肠黏膜组织内,TRPV5、TRPV6表达程度与组织增殖程度以及息肉恶变的危险性之间具有相关关系,TRPV5、TRPV6可能在结肠腺癌发生、发展过程中扮演着重要的角色.展开更多
A 17-year-old female presented with rectal bleeding from an ulcerated sigmoid mass in 1994.Initial pathological evaluation revealed a rare clear cell neoplasm of the colon,possibly originating from kidneys,adrenals,lu...A 17-year-old female presented with rectal bleeding from an ulcerated sigmoid mass in 1994.Initial pathological evaluation revealed a rare clear cell neoplasm of the colon,possibly originating from kidneys,adrenals,lung or a gynecologic source as a metastatic lesion.Extensive imaging studies were negative,and over the next 15 years,she remained well with no recurrence.The original resected neoplasm was reviewed and reclassified as a perivascular epithelioid cell neoplasm (PEComa).Although the long-term natural history of PEComas requires definition,increased clinical and pathological awareness should lead to increased recognition of an apparently rare type of colonic neoplasm that likely occurs more often than is currently appreciated.展开更多
目的构建一个基于Notch通路相关基因的结肠腺癌预后模型并通过列线图绘制进行验证。方法下载TCGA结肠腺癌患者的mRNA表达量和临床病理资料。GSEA(gene set enrichment analysis)分析Notch信号通路相关基因集,筛选癌与癌旁组织中差异表...目的构建一个基于Notch通路相关基因的结肠腺癌预后模型并通过列线图绘制进行验证。方法下载TCGA结肠腺癌患者的mRNA表达量和临床病理资料。GSEA(gene set enrichment analysis)分析Notch信号通路相关基因集,筛选癌与癌旁组织中差异表达的基因。单因素与多因素Cox比例回归模型进行预后相关mRNAs的筛选,并构建基于mRNAs表达谱的预后模型和列线图,通过生存分析C-index、ROC曲线和校准曲线评估其预测价值。从GEO数据库中下载验证队列GSE29621,对预后模型预测患者预后的有效性进行验证。使用在线网站人类蛋白图谱(HPA)对预后模型内基因进行蛋白表达情况验证。结果从TCGA中下载444例结肠腺癌患者mRNA表达数据和临床病理资料,排除临床资料不全的患者信息,最终纳入385例患者的信息作为研究对象。从GSEA中3个Notch信号通路基因集中获取Notch相关基因,并与TCGA表达数据结合,得到Notch相关基因的表达量,最后进行Notch相关基因的差异基因的筛选。使用GSEA在结肠腺癌mRNAs表达谱中筛选出391个Notch信号通路相关差异表达基因(P<0.05)。利用单变量Cox回归分析筛选出14个结肠腺癌预后相关Notch通路基因,进一步多变量Cox回归分析构建出5种mRNAs(CDHR2,KRT8P12,NEURL1B,SELE,FSTL3)组成的预后模型。ROC曲线和生存分析显示,高Notch通路相关基因风险评分与较差的生存结果显著相关(AUC=0.748,P<0.05)。Notch通路相关的基因评分被证明是一个独立的预后因素。构建了具有临床病理特征和Notch通路相关基因评分的列线图,进一步预测结肠腺癌患者的预后,在C-index、ROC曲线和校准曲线上也表现良好(C-index=0.794,AUC=0.969)。结论构建的包含5个Notch信号通路相关基因的结肠腺癌预后模型具有良好的预后预测效果,有望作为评估结肠腺癌患者预后的指标。展开更多
基金Supported by The Buddhist Dalin Tzu-Chi General Hospital
文摘Extranodal natural killer/T-cell lymphoma(ENKTL) is a distinct subtype of non-Hodgkin's lymphoma and is rare in the colon.Synchronous adenocarcinoma and ENKTL of the colon has not been reported in the literature.In the present study,we report a 63-year-old male who suffered from intermittent bloody stools for 2 mo.He did not have fever,body weight loss or night sweat.Endoscopic and imaging studies revealed a 4.5-cm ulcerative mass in the ascending colon and a 3.0-cm polypoid,easy bleeding mass in the sigmoid colon,respectively.Thought to have double carcinoma of the colon,he received simultaneous right hemicolectomy and sigmoidectomy.The pathological diagnosis was a synchronous ENKTL(ascending colon) and adenocarcinoma(sigmoid colon).The literature on synchronous adenocarcinoma and malignant lymphoma of the colon was also reviewed.
文摘目的旨在构建结肠腺癌(colonic adenocarcinoma,COAD)中铜死亡相关基因(Cuproptosis-related genes,CRGs)的预后模型,探讨CRGs在COAD中的表达与预后的关系。方法从TCGA数据库下载了正常和COAD患者的转录组和临床数据,并从GEO数据库下载数据集GSE39582作为外部验证集。基于铜死亡相关基因表达量,对TCGA数据进行共识聚类以确定新的肿瘤亚型。采用单因素Cox回归分析、LASSO回归分析筛选预后基因并构建预后风险模型。并通过Kaplan-Meier生存分析、受试者工作特征(receiver operating characteristic,ROC)曲线下的面积(area under the curve,AUC)和一致性指数(concordance index,C-index)对模型进行评价。用GSE44001数据集对模型进行外部验证。基于风险评分及临床因素构建列线图,预测患者的生存率。并进行免疫浸润、GSEA分析、免疫检查点、药敏分析、肿瘤微环境和干细胞指数的研究,系统地阐述COAD中CRGs和免疫的相关性。结果21个CRGs在COAD组织及正常组织中差异表达。单变量Cox回归及Lasso回归分析确定了五个预后相关基因(SDHB,CCS,DLAT,FDX1,CP)并构建模型。Kaplan-Meier分析表明,高风险患者OS显著低于低风险患者(P<0.01),并在GEO队列中取得了验证(P<0.05)。风险模型的AUC值为0.734,表明预后值有统计学意义,模型具有一定的准确性。一致性分析和ROC结果表明,列线图模型有较好的预后预测准确性。这5个CRGs主要参与线粒体、免疫应答和代谢途径,是COAD预后的独立因素,并与免疫细胞浸润、免疫检查点以及肿瘤微环境和干细胞指数密切相关。结论构建了5个铜死亡相关基因的结肠腺癌预后模型,有可能作为未来临床实践中有效的预后标志物。
文摘目的:通过观察正常结肠组织及各类型结肠腺瘤息肉和结肠癌组织类型中瞬时受体阳离子通道亚家族V成员5(transient receptor potential cation channel,subfamily V,member5,TRPV5)、TRPV6表达情况,研究TRPV5、TRPV6的表达变化是否与结肠腺瘤-腺癌的演变过程相关,探讨其在人类结肠腺瘤-腺癌形成过程中的作用.方法:取肠镜活检或手术切除的增生性息肉、结肠腺瘤(免疫组织化学40例)、结肠癌标本各20例及正常对照肠黏膜20例.采用实时荧光定量PCR技术、Western blot技术及免疫组织化学技术检测并比较正常结肠组织、结肠腺瘤及结肠癌组织中TRPV5、TRPV6m R N A及其相应的蛋白含量的表达,同时通过免疫组织化学测定组织细胞Ki-67来观察TRPV5、TRPV6表达与上述组织细胞增殖的关系.结果:(1)TRPV5、TRPV6在正常结肠组织、结肠息肉组织及结肠癌组织中均有表达,且主要表达部位均在细胞胞浆;Ki-67表达在TRPV5或TRPV6表达增高时亦增高(P<0.05);(2)T R P V5、T R P V6的蛋白及m R N A在正常肠黏膜及非腺瘤息肉中呈弱阳性表达或不表达(P>0.05),在结肠腺瘤及结肠腺癌中高表达(P<0.05),且与组织类型具有直线相关性(P<0.05);(3)在结肠黏膜组织内,TRPV5表达量低于TRPV6(P<0.05).结论:在结肠黏膜组织内,TRPV5、TRPV6均有不同程度表达,特别在结肠腺瘤及结肠腺癌中呈高表达状态,同时Ki-67表达亦相应增高.提示在结肠黏膜组织内,TRPV5、TRPV6表达程度与组织增殖程度以及息肉恶变的危险性之间具有相关关系,TRPV5、TRPV6可能在结肠腺癌发生、发展过程中扮演着重要的角色.
文摘A 17-year-old female presented with rectal bleeding from an ulcerated sigmoid mass in 1994.Initial pathological evaluation revealed a rare clear cell neoplasm of the colon,possibly originating from kidneys,adrenals,lung or a gynecologic source as a metastatic lesion.Extensive imaging studies were negative,and over the next 15 years,she remained well with no recurrence.The original resected neoplasm was reviewed and reclassified as a perivascular epithelioid cell neoplasm (PEComa).Although the long-term natural history of PEComas requires definition,increased clinical and pathological awareness should lead to increased recognition of an apparently rare type of colonic neoplasm that likely occurs more often than is currently appreciated.
文摘目的构建一个基于Notch通路相关基因的结肠腺癌预后模型并通过列线图绘制进行验证。方法下载TCGA结肠腺癌患者的mRNA表达量和临床病理资料。GSEA(gene set enrichment analysis)分析Notch信号通路相关基因集,筛选癌与癌旁组织中差异表达的基因。单因素与多因素Cox比例回归模型进行预后相关mRNAs的筛选,并构建基于mRNAs表达谱的预后模型和列线图,通过生存分析C-index、ROC曲线和校准曲线评估其预测价值。从GEO数据库中下载验证队列GSE29621,对预后模型预测患者预后的有效性进行验证。使用在线网站人类蛋白图谱(HPA)对预后模型内基因进行蛋白表达情况验证。结果从TCGA中下载444例结肠腺癌患者mRNA表达数据和临床病理资料,排除临床资料不全的患者信息,最终纳入385例患者的信息作为研究对象。从GSEA中3个Notch信号通路基因集中获取Notch相关基因,并与TCGA表达数据结合,得到Notch相关基因的表达量,最后进行Notch相关基因的差异基因的筛选。使用GSEA在结肠腺癌mRNAs表达谱中筛选出391个Notch信号通路相关差异表达基因(P<0.05)。利用单变量Cox回归分析筛选出14个结肠腺癌预后相关Notch通路基因,进一步多变量Cox回归分析构建出5种mRNAs(CDHR2,KRT8P12,NEURL1B,SELE,FSTL3)组成的预后模型。ROC曲线和生存分析显示,高Notch通路相关基因风险评分与较差的生存结果显著相关(AUC=0.748,P<0.05)。Notch通路相关的基因评分被证明是一个独立的预后因素。构建了具有临床病理特征和Notch通路相关基因评分的列线图,进一步预测结肠腺癌患者的预后,在C-index、ROC曲线和校准曲线上也表现良好(C-index=0.794,AUC=0.969)。结论构建的包含5个Notch信号通路相关基因的结肠腺癌预后模型具有良好的预后预测效果,有望作为评估结肠腺癌患者预后的指标。
