Objective:To study the effect of bone marrow mesenchymal stem cell(BMSCs) on angiogenesis and inflammation in rabbits with acute cornea alkali burn.Methods:New Zealand White Rabbit were chosen,while model of cornea al...Objective:To study the effect of bone marrow mesenchymal stem cell(BMSCs) on angiogenesis and inflammation in rabbits with acute cornea alkali burn.Methods:New Zealand White Rabbit were chosen,while model of cornea alkali bum was made and divided into BMSCs group and control group.Rabbits in the BMSCs group were injected with BMSCs and those in the control group received PBS Solution via ear vein.Cornea turbidity and the angiogenesis area were measured at 20 d,40 d,60 d.The expression of angiogenic and inflammation response cytokines were measured at 60 d.Results:At 20 d,40 d,60 d,the cornea turbidity in BMSCs group was lower than that in control group,and the angiogenesis area in BMSCs group was smaller than that in control group;At 60 d,the mRNA expression of HIF-1 α,VEGF,MMP2,MMP9,TLR2,TLR4,IL-1 α,IL-1 β,TNF-α,ICAM-1 in cornea of BMSCs group was substantially Lower than that of control group,while BMSCs group was substantially higher than control group on mRNA expression of PEDE TIMP1,TIMP2.Conclusions:BMSCs has inhibitory effect on angiogenesis and inflammatory response so that it is conducive to the healing of cornea alkali burn.展开更多
文摘我们自1990年4月至1993年5月共治疗8例广泛头皮深度烧伤伴有颅骨全层坏死的病例,早期经头颅 CT 及^(99m)锝同位素颅骨扫描检查明确颅内损伤及颅骨坏死范围后,采用吻合血管的血流量大的肌皮瓣或游离皮瓣移植,在保留坏死颅骨情况下Ⅰ期修复创面。8例中4例创面Ⅰ期愈合,另3例在已愈合皮瓣保护下多次清创去除死骨。经术后1～4年随访表明,坏死颅骨作为修复支架保留,避免了颅骨缺损可能造成的并发症及后遗症。本组仅1例于入院后24 h 因颅脑严重损伤而死亡。我们所介绍的这一方法为类似严重病例的治疗提供了有效手段。
文摘Objective:To study the effect of bone marrow mesenchymal stem cell(BMSCs) on angiogenesis and inflammation in rabbits with acute cornea alkali burn.Methods:New Zealand White Rabbit were chosen,while model of cornea alkali bum was made and divided into BMSCs group and control group.Rabbits in the BMSCs group were injected with BMSCs and those in the control group received PBS Solution via ear vein.Cornea turbidity and the angiogenesis area were measured at 20 d,40 d,60 d.The expression of angiogenic and inflammation response cytokines were measured at 60 d.Results:At 20 d,40 d,60 d,the cornea turbidity in BMSCs group was lower than that in control group,and the angiogenesis area in BMSCs group was smaller than that in control group;At 60 d,the mRNA expression of HIF-1 α,VEGF,MMP2,MMP9,TLR2,TLR4,IL-1 α,IL-1 β,TNF-α,ICAM-1 in cornea of BMSCs group was substantially Lower than that of control group,while BMSCs group was substantially higher than control group on mRNA expression of PEDE TIMP1,TIMP2.Conclusions:BMSCs has inhibitory effect on angiogenesis and inflammatory response so that it is conducive to the healing of cornea alkali burn.
