摘要
Objective:To study the effect of bone marrow mesenchymal stem cell(BMSCs) on angiogenesis and inflammation in rabbits with acute cornea alkali burn.Methods:New Zealand White Rabbit were chosen,while model of cornea alkali bum was made and divided into BMSCs group and control group.Rabbits in the BMSCs group were injected with BMSCs and those in the control group received PBS Solution via ear vein.Cornea turbidity and the angiogenesis area were measured at 20 d,40 d,60 d.The expression of angiogenic and inflammation response cytokines were measured at 60 d.Results:At 20 d,40 d,60 d,the cornea turbidity in BMSCs group was lower than that in control group,and the angiogenesis area in BMSCs group was smaller than that in control group;At 60 d,the mRNA expression of HIF-1 α,VEGF,MMP2,MMP9,TLR2,TLR4,IL-1 α,IL-1 β,TNF-α,ICAM-1 in cornea of BMSCs group was substantially Lower than that of control group,while BMSCs group was substantially higher than control group on mRNA expression of PEDE TIMP1,TIMP2.Conclusions:BMSCs has inhibitory effect on angiogenesis and inflammatory response so that it is conducive to the healing of cornea alkali burn.
