Background:Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus affected more people than SARS-CoV-1 and related coronaviruses.Human genetic factors,besides respiratory droplets and direct exposure to the ...Background:Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus affected more people than SARS-CoV-1 and related coronaviruses.Human genetic factors,besides respiratory droplets and direct exposure to the virus,are found highly responsible for transmitting SARS-CoV-2 infection.Aim:The objective of this study was to determine the plasma levels of the TMPRSS2 gene and its role in SARS-CoV-2 susceptibility.Methodology:A total of 100 patients,i.e.50 SARS-CoV-2 positive patients as the case group and 50 SARS-CoV-2 negative samples as the control group,were selected randomly and included in this case-control study to determine the association between TMPRSS2 gene and susceptibility to SARS-CoV-2 infection and severity of coronavirus disease 2019(COVID-19).The TMPRSS2 levels of case and control samples were measured through an enzyme-linked immunosorbent assay(ELISA).Following the genomic DNA extraction,a set of reverse and forward primers of human TMPRSS2 gene primers were used for the amplification of the TMPRSS2 gene.Results:In the control group,the ratio of men to women was more or less the same while in the case group 62%of the population were women.The TMPRSS2 level was found to be 4.70±7.7 ng/ml in case samples while it was 4.73±5.7 ng/ml in control samples.Conclusion:The levels of TMPRSS2 in plasma samples of both controls and cases were found to be the same indicating that the entry of SARS-CoV-2 is not dependent on the plasma levels of this protein.展开更多
A recent study by Brevini et al.was published in Nature and proposed a critical role of farnesoid X receptor(FXR)signaling in controlling ACE2 expression.Inhibitors of FXR signaling,ursodeoxycholic acid(UDCA)and z-gug...A recent study by Brevini et al.was published in Nature and proposed a critical role of farnesoid X receptor(FXR)signaling in controlling ACE2 expression.Inhibitors of FXR signaling,ursodeoxycholic acid(UDCA)and z-guggulsterone(ZGG),have been proven to reduce ACE2 expression and susceptibility to SARS-CoV-2 infection in human tissues,providing an innovative strategy for SARS-CoV-2 treatment and prevention.展开更多
Since the outbreak at the end of 2019,SARS-CoV-2 has been spreading around the world for more than one year.Scientists have been intensely conducting research on this newly emerged coronavirus and the disease caused b...Since the outbreak at the end of 2019,SARS-CoV-2 has been spreading around the world for more than one year.Scientists have been intensely conducting research on this newly emerged coronavirus and the disease caused by it.Angiotensin-converting enzyme 2(ACE2),as a receptor mediating the cellular entry of SARSCoV-2,has become a hot spot for researchers.Here,we summarized the recent progresses on the function,expression and distribution characteristics of ACE2 in human body and among populations.We further discussed the interaction mechanism of ACE2 and SARS-CoV-2 S protein,focusing on key residues that effect interaction and binding ability of SARS-CoV-2 variants.This will facilitate researchers to better understand SARS-CoV-2 infection and transmission route,adaptation mechanism,and designing treatment strategies.展开更多
基金supported by grants from the Shaheed Zulfikar Ali Bhutto Institute of Science and Technology(SZABIST)University,Karachi,Pakistan.
文摘Background:Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus affected more people than SARS-CoV-1 and related coronaviruses.Human genetic factors,besides respiratory droplets and direct exposure to the virus,are found highly responsible for transmitting SARS-CoV-2 infection.Aim:The objective of this study was to determine the plasma levels of the TMPRSS2 gene and its role in SARS-CoV-2 susceptibility.Methodology:A total of 100 patients,i.e.50 SARS-CoV-2 positive patients as the case group and 50 SARS-CoV-2 negative samples as the control group,were selected randomly and included in this case-control study to determine the association between TMPRSS2 gene and susceptibility to SARS-CoV-2 infection and severity of coronavirus disease 2019(COVID-19).The TMPRSS2 levels of case and control samples were measured through an enzyme-linked immunosorbent assay(ELISA).Following the genomic DNA extraction,a set of reverse and forward primers of human TMPRSS2 gene primers were used for the amplification of the TMPRSS2 gene.Results:In the control group,the ratio of men to women was more or less the same while in the case group 62%of the population were women.The TMPRSS2 level was found to be 4.70±7.7 ng/ml in case samples while it was 4.73±5.7 ng/ml in control samples.Conclusion:The levels of TMPRSS2 in plasma samples of both controls and cases were found to be the same indicating that the entry of SARS-CoV-2 is not dependent on the plasma levels of this protein.
基金the National Key R&D Program of China(2022YFA1105200)the Chinese National Natural Science Funds(31870902,32070907,and 31871405)+1 种基金the National Science Fund for Distinguished Young Scholars(32125016)the Jiangsu National Science Foundation(19KJA550003).
文摘A recent study by Brevini et al.was published in Nature and proposed a critical role of farnesoid X receptor(FXR)signaling in controlling ACE2 expression.Inhibitors of FXR signaling,ursodeoxycholic acid(UDCA)and z-guggulsterone(ZGG),have been proven to reduce ACE2 expression and susceptibility to SARS-CoV-2 infection in human tissues,providing an innovative strategy for SARS-CoV-2 treatment and prevention.
基金supported by the China Postdoctoral Science Fund(No.2019M664012 and No.2020T130135ZX).
文摘Since the outbreak at the end of 2019,SARS-CoV-2 has been spreading around the world for more than one year.Scientists have been intensely conducting research on this newly emerged coronavirus and the disease caused by it.Angiotensin-converting enzyme 2(ACE2),as a receptor mediating the cellular entry of SARSCoV-2,has become a hot spot for researchers.Here,we summarized the recent progresses on the function,expression and distribution characteristics of ACE2 in human body and among populations.We further discussed the interaction mechanism of ACE2 and SARS-CoV-2 S protein,focusing on key residues that effect interaction and binding ability of SARS-CoV-2 variants.This will facilitate researchers to better understand SARS-CoV-2 infection and transmission route,adaptation mechanism,and designing treatment strategies.
