建立高效液相色谱法测定化妆品中对羟基苯乙酮、对茴香酸和辛酰羟肟酸含量的分析方法。样品经体积分数为80%的甲醇溶液超声提取,以0.1%(体积分数)磷酸溶液-乙腈作为流动相进行梯度洗脱,采用Osaka Soda Capcell Pak MG C18色谱柱(250 mm&...建立高效液相色谱法测定化妆品中对羟基苯乙酮、对茴香酸和辛酰羟肟酸含量的分析方法。样品经体积分数为80%的甲醇溶液超声提取,以0.1%(体积分数)磷酸溶液-乙腈作为流动相进行梯度洗脱,采用Osaka Soda Capcell Pak MG C18色谱柱(250 mm×4.6 mm,5μm)进行分离,流量为1.0 mL/min,柱温为30℃,检测波长为210 nm,色谱峰面积外标法定量。对羟基苯乙酮、对茴香酸和辛酰羟肟酸的质量浓度在2.5~60 mg/L范围内与对应色谱峰面积线性关系良好,相关系数均不小于0.9998,方法检出限均不大于0.22 mg/L。低、中、高三个浓度水平的加标回收率为85.8%~111.1%,测定结果的相对标准偏差为0.5%~5.9%(n=6)。该方法操作简便,适用于化妆品中3种物质的测定。展开更多
A novel ligand N-4-hydroxyacetophenone isonicotinoyl hydrazone and its manganese(II) and nickel(II) metal complexes have been synthesized. The synthesized Schiff base and its metal complexes have been characterized by...A novel ligand N-4-hydroxyacetophenone isonicotinoyl hydrazone and its manganese(II) and nickel(II) metal complexes have been synthesized. The synthesized Schiff base and its metal complexes have been characterized by physical state determination, melting point and solubility measurements in different solvents, infrared, proton nuclear magnetic resonance, mass spectrometric and powder X-ray spectroscopic techniques. The thermal properties of the prepared compounds were obtained from TG/DTG measurements. On the basis of the analytical techniques, the ligand was found to be bidentate in nature coordinating to the metal ions through the azomethine nitrogen and carbonyl oxygen atoms leading to distorted octahedral geometries of the metal complexes which were modeled using MM2 force field. The ligand and its metal(II) complexes were evaluated for antifungal activity against <i>Aspergillus fumigatus, Aspergillus niger, Candida albicans and Rhizopus stolonifera.</i> The antifungal evaluation results revealed an enhanced activity upon coordination of the ligand with the metal(II) ions. The activity of the metal complex to the tested fungal strains was in the order Ni(II) > Mn(II).展开更多
文摘A novel ligand N-4-hydroxyacetophenone isonicotinoyl hydrazone and its manganese(II) and nickel(II) metal complexes have been synthesized. The synthesized Schiff base and its metal complexes have been characterized by physical state determination, melting point and solubility measurements in different solvents, infrared, proton nuclear magnetic resonance, mass spectrometric and powder X-ray spectroscopic techniques. The thermal properties of the prepared compounds were obtained from TG/DTG measurements. On the basis of the analytical techniques, the ligand was found to be bidentate in nature coordinating to the metal ions through the azomethine nitrogen and carbonyl oxygen atoms leading to distorted octahedral geometries of the metal complexes which were modeled using MM2 force field. The ligand and its metal(II) complexes were evaluated for antifungal activity against <i>Aspergillus fumigatus, Aspergillus niger, Candida albicans and Rhizopus stolonifera.</i> The antifungal evaluation results revealed an enhanced activity upon coordination of the ligand with the metal(II) ions. The activity of the metal complex to the tested fungal strains was in the order Ni(II) > Mn(II).
