BACKGROUND Pegylated liposomal doxorubicin(PLD)uses the hydrophilic layer of liposomes to reach the sweat on the skin surface or accumulate in the sweat glands,producing toxic free radicals and oxidative damage,result...BACKGROUND Pegylated liposomal doxorubicin(PLD)uses the hydrophilic layer of liposomes to reach the sweat on the skin surface or accumulate in the sweat glands,producing toxic free radicals and oxidative damage,resulting in hand-foot syndrome(HFS).Regional cooling can induce vasoconstriction to reduce the release of drugs in the limbs and reduce the accumulation of drugs in sweat glands;thus,decreasing the incidence and severity of HFS.AIM To study the efficacy of cooling patches to prevent HFS caused by PLD in the short-term.METHODS This is a retrospective cohort study.Female breast cancer patients(n=101)who were treated with PLD in two breast wards at our department from February 2020 to February 2021 were enrolled in the study and were randomly divided into the cooling group(51 patients)and the control group(50 patients).Patients in the control group only received routine care,while the patients in the cooling group applied cooling patches,based on routine care,to the palm and back of the hands 15 min before chemotherapy infusion for 10 h.All patients took a corresponding dose of dexamethasone orally one day before chemotherapy,on the day of chemotherapy,and one day after chemotherapy.SPSS23.0 version was used to analyze the data in this study.The occurrence and severity of HFS was analyzed by the Mann-Whitney U test,and scores were analyzed by the Student’s t test or Wilcoxon rank-sum test.A P value<0.05 was regarded as statistically significant.RESULTS In this study,neither group of patients developed Grade 3 HFS.In the control group,the incidence of Grade 1 HFS and Grade 2 HFS was 38%and 2%,respectively.However,in the cooling group,only one person developed Grade 1 HFS(2%),and none of the patients developed Grade 2 HFS.These findings showed that cooling patches can effectively reduce the frequency and severity of HFS(P<0.0001)in the short-term.Before the fourth chemotherapy cycle,although general self-efficacy scale scores in the cooling group were low,they were still significantly higher than those in th展开更多
A new automatic sample solution introduction system for miniature simultaneous microwave plasma torch（MPT） atomic emission spectrometer was developed. The operating parameters were optimized. The detection limits of...A new automatic sample solution introduction system for miniature simultaneous microwave plasma torch（MPT） atomic emission spectrometer was developed. The operating parameters were optimized. The detection limits of the spectrometer with an ultrasonic nebulizer for Ag, Al, Ba, Ca, Cr, Cu, Fe, Mg, Mn, Sr, and V are 5-10 times lower than those obtained with a pneumatic nebulizer and are also lower than those obtained by a Model JX-lOlO MPT spectrometer. Two practical samples were analyzed to test the reliability and sensitivity of the system.展开更多
为建立检测F亚群禽白血病病毒(ALV-F)的SYBR Green I荧光定量PCR方法,本研究以ALV-F分离株FGD1803基因组为模板,PCR扩增其env基因,构建重组质粒pMD18-T-F作为标准品。利用该重组质粒建立了SYBR Green I荧光定量PCR方法(qPCR)的标准曲线...为建立检测F亚群禽白血病病毒(ALV-F)的SYBR Green I荧光定量PCR方法,本研究以ALV-F分离株FGD1803基因组为模板,PCR扩增其env基因,构建重组质粒pMD18-T-F作为标准品。利用该重组质粒建立了SYBR Green I荧光定量PCR方法(qPCR)的标准曲线,并经反应条件优化建立了检测ALV-F的qPCR方法。建立的标准曲线结果显示,重组质粒标准品的拷贝数与Ct值呈良好的线性关系,R^(2)为0.993,扩增效率为1.03。该方法各反应条件的优化结果为10μmol/L上下游引物各0.5μL,退火温度为55℃。特异性试验结果显示,该方法仅能特异性检测ALV-F,而对ALV-A、ALV-B、ALV-J、ALV-K、ALV-E、网状内皮增生病病毒、马立克氏病病毒、禽流感病毒、新城疫病毒、鸡支气管炎病毒等禽源病毒无交叉反应;敏感性试验结果显示,该方法对质粒标准品的最低检测限为1.16×10^(2)拷贝/μL,敏感性是常规PCR方法的100倍;以3个不同浓度重组质粒标准品为模板进行重复性试验,结果显示批内和批间重复性试验变异系数均小于5%;利用该方法与病毒培养方法分别检测30份七彩山鸡血浆样品(21份ALV阳性,9份阴性)的细胞培养物,结果显示该qPCR方法的阳性检出率为33.3%(10/30),常规PCR方法的阳性检出率为20.0%(6/30),病毒分离方法的阳性检出率为13.3%(4/30),该qPCR方法与病毒分离方法的符合率为80.0%。本研究在国内首次建立了用于ALV-F检测的qPCR方法,该方法具有良好的特异性、敏感性和重复性,可以用于临床样品的检测。为该病毒的检测提供了技术支撑。展开更多
文摘BACKGROUND Pegylated liposomal doxorubicin(PLD)uses the hydrophilic layer of liposomes to reach the sweat on the skin surface or accumulate in the sweat glands,producing toxic free radicals and oxidative damage,resulting in hand-foot syndrome(HFS).Regional cooling can induce vasoconstriction to reduce the release of drugs in the limbs and reduce the accumulation of drugs in sweat glands;thus,decreasing the incidence and severity of HFS.AIM To study the efficacy of cooling patches to prevent HFS caused by PLD in the short-term.METHODS This is a retrospective cohort study.Female breast cancer patients(n=101)who were treated with PLD in two breast wards at our department from February 2020 to February 2021 were enrolled in the study and were randomly divided into the cooling group(51 patients)and the control group(50 patients).Patients in the control group only received routine care,while the patients in the cooling group applied cooling patches,based on routine care,to the palm and back of the hands 15 min before chemotherapy infusion for 10 h.All patients took a corresponding dose of dexamethasone orally one day before chemotherapy,on the day of chemotherapy,and one day after chemotherapy.SPSS23.0 version was used to analyze the data in this study.The occurrence and severity of HFS was analyzed by the Mann-Whitney U test,and scores were analyzed by the Student’s t test or Wilcoxon rank-sum test.A P value<0.05 was regarded as statistically significant.RESULTS In this study,neither group of patients developed Grade 3 HFS.In the control group,the incidence of Grade 1 HFS and Grade 2 HFS was 38%and 2%,respectively.However,in the cooling group,only one person developed Grade 1 HFS(2%),and none of the patients developed Grade 2 HFS.These findings showed that cooling patches can effectively reduce the frequency and severity of HFS(P<0.0001)in the short-term.Before the fourth chemotherapy cycle,although general self-efficacy scale scores in the cooling group were low,they were still significantly higher than those in th
文摘A new automatic sample solution introduction system for miniature simultaneous microwave plasma torch（MPT） atomic emission spectrometer was developed. The operating parameters were optimized. The detection limits of the spectrometer with an ultrasonic nebulizer for Ag, Al, Ba, Ca, Cr, Cu, Fe, Mg, Mn, Sr, and V are 5-10 times lower than those obtained with a pneumatic nebulizer and are also lower than those obtained by a Model JX-lOlO MPT spectrometer. Two practical samples were analyzed to test the reliability and sensitivity of the system.
文摘为建立检测F亚群禽白血病病毒(ALV-F)的SYBR Green I荧光定量PCR方法,本研究以ALV-F分离株FGD1803基因组为模板,PCR扩增其env基因,构建重组质粒pMD18-T-F作为标准品。利用该重组质粒建立了SYBR Green I荧光定量PCR方法(qPCR)的标准曲线,并经反应条件优化建立了检测ALV-F的qPCR方法。建立的标准曲线结果显示,重组质粒标准品的拷贝数与Ct值呈良好的线性关系,R^(2)为0.993,扩增效率为1.03。该方法各反应条件的优化结果为10μmol/L上下游引物各0.5μL,退火温度为55℃。特异性试验结果显示,该方法仅能特异性检测ALV-F,而对ALV-A、ALV-B、ALV-J、ALV-K、ALV-E、网状内皮增生病病毒、马立克氏病病毒、禽流感病毒、新城疫病毒、鸡支气管炎病毒等禽源病毒无交叉反应;敏感性试验结果显示,该方法对质粒标准品的最低检测限为1.16×10^(2)拷贝/μL,敏感性是常规PCR方法的100倍;以3个不同浓度重组质粒标准品为模板进行重复性试验,结果显示批内和批间重复性试验变异系数均小于5%;利用该方法与病毒培养方法分别检测30份七彩山鸡血浆样品(21份ALV阳性,9份阴性)的细胞培养物,结果显示该qPCR方法的阳性检出率为33.3%(10/30),常规PCR方法的阳性检出率为20.0%(6/30),病毒分离方法的阳性检出率为13.3%(4/30),该qPCR方法与病毒分离方法的符合率为80.0%。本研究在国内首次建立了用于ALV-F检测的qPCR方法,该方法具有良好的特异性、敏感性和重复性,可以用于临床样品的检测。为该病毒的检测提供了技术支撑。
基金supported by the National Natural Science Foundation of China(No.31571179 and 31371165)the Natural Science Foundation of Fujian Province,China(No.2015J01313)
