In the paper,we discuss the development of the multigap resistive plate chamber time-of-fight(TOF)technology and the production of the solenoidal tracker at RHIC(STAR)TOF detector in China at the beginning of the twen...In the paper,we discuss the development of the multigap resistive plate chamber time-of-fight(TOF)technology and the production of the solenoidal tracker at RHIC(STAR)TOF detector in China at the beginning of the twenty-frst century.Subsequently,recent experimental results from the frst beam energy scan program(BES-I)at the Relativistic Heavy Ion Collider(RHIC)pertaining to measurements of collectivity,chirality,criticality,global polarization,strangeness,heavy favor,dilepton and light nuclei productions are reviewed.展开更多
Shikimic acid(SA) is the key synthetic material for the chemical synthesis of Oseltamivir, which is prescribed as the front-line treatment for serious cases of influenza. Multi-gene expression vector can be used for e...Shikimic acid(SA) is the key synthetic material for the chemical synthesis of Oseltamivir, which is prescribed as the front-line treatment for serious cases of influenza. Multi-gene expression vector can be used for expressing the plurality of the genes in one plasmid, so it is widely applied to increase the yield of metabolites. In the present study, on the basis of a shikimate kinase genetic defect strain Escherichia coli BL21(?aro L/aro K, DE3), the key enzyme genes aro G, aro B, tkt A and aro E of SA pathway were co-expressed and compared systematically by constructing a series of multi-gene expression vectors. The results showed that different gene co-expression combinations(two, three or four genes) or gene orders had different effects on the production of SA. SA production of the recombinant BL21-GBAE reached to 886.38 mg·L^(-1), which was 17-fold(P < 0.05) of the parent strain BL21(?aro L/aro K, DE3).展开更多
Objective： To evaluate the association between p53 codon 72 polymorphism （R72P） and the risk ofcolorectal liver metastases. Methods： The p53 R72P genotype was identified by polymerase chain reaction-restriction fr...Objective： To evaluate the association between p53 codon 72 polymorphism （R72P） and the risk ofcolorectal liver metastases. Methods： The p53 R72P genotype was identified by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method in 78 consecutive colorectal cancer patients with liver metastases and 214 age- and sex-matched cases with nonmetastatic colorectal cancer. Results： The R allele of the p53 R72P polymorphism was more frequently found in metastatic cases than in nonmetastatic cases （P=0.075）. Carriers of the 72R allele had a 2.25-fold （95% CI （confidence interval）=1.05-4.83） increased risk of liver metastases. On the stratification analysis, 72R-carrying genotype conferred a 3.46-fold （95% CI=1.02-11.72） and a 1.05-fold （95% CI=0.36-3.08） increased risk of liver metastases for p53 overexpression-positive and negative colorectal cancers, respectively. Conclusion： These results demonstrate for the first time that the 72R allele of the p53 polymorphism has an increased risk for liver metastases in colorectal cancers positive for p53 overexpression.展开更多
Abnormally enhanced de novo lipid biosynthesis has been increasingly realized to play crucial roles in the initiation and progression of varieties of cancers including breast cancer.However,the mechanisms underlying t...Abnormally enhanced de novo lipid biosynthesis has been increasingly realized to play crucial roles in the initiation and progression of varieties of cancers including breast cancer.However,the mechanisms underlying the dysregulation of lipid biosynthesis in breast cancer remain largely unknown.Here,we reported that seryl tRNA synthetase(SerRS),a key enzyme for protein biosynthesis,could translocate into the nucleus in a glucose-dependent manner to suppress key genes involved in the de novo lipid biosynthesis.In normal mammary gland epithelial cells glucose can promote the nuclear translocation of SerRS by increasing the acetylation of SerRS at lysine 323.In SerRS knock-in mice bearing acetylation-defective lysine to arginine mutation.展开更多
基金National Key Research and Development Program of China(No.2022YFA1604900)National Natural Science Foundation of China(No.12025501)Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB34000000)。
文摘In the paper,we discuss the development of the multigap resistive plate chamber time-of-fight(TOF)technology and the production of the solenoidal tracker at RHIC(STAR)TOF detector in China at the beginning of the twenty-frst century.Subsequently,recent experimental results from the frst beam energy scan program(BES-I)at the Relativistic Heavy Ion Collider(RHIC)pertaining to measurements of collectivity,chirality,criticality,global polarization,strangeness,heavy favor,dilepton and light nuclei productions are reviewed.
基金supported by National Important Special Foundation of the New Drug Development(2012ZX 09301002-003 and 2014ZX09201001-012)Shanghai Innovation Action Plan of Science and Technology(14431905900)
文摘Shikimic acid(SA) is the key synthetic material for the chemical synthesis of Oseltamivir, which is prescribed as the front-line treatment for serious cases of influenza. Multi-gene expression vector can be used for expressing the plurality of the genes in one plasmid, so it is widely applied to increase the yield of metabolites. In the present study, on the basis of a shikimate kinase genetic defect strain Escherichia coli BL21(?aro L/aro K, DE3), the key enzyme genes aro G, aro B, tkt A and aro E of SA pathway were co-expressed and compared systematically by constructing a series of multi-gene expression vectors. The results showed that different gene co-expression combinations(two, three or four genes) or gene orders had different effects on the production of SA. SA production of the recombinant BL21-GBAE reached to 886.38 mg·L^(-1), which was 17-fold(P < 0.05) of the parent strain BL21(?aro L/aro K, DE3).
基金Project supported by the National Natural Science Foundation of China (No. 30470791) the Medical Science and Technology Research Foundation for the 11th Five-Year Program of People's Liberation Army, Nanjing Branch, China (No. 06MA27)
文摘Objective： To evaluate the association between p53 codon 72 polymorphism （R72P） and the risk ofcolorectal liver metastases. Methods： The p53 R72P genotype was identified by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method in 78 consecutive colorectal cancer patients with liver metastases and 214 age- and sex-matched cases with nonmetastatic colorectal cancer. Results： The R allele of the p53 R72P polymorphism was more frequently found in metastatic cases than in nonmetastatic cases （P=0.075）. Carriers of the 72R allele had a 2.25-fold （95% CI （confidence interval）=1.05-4.83） increased risk of liver metastases. On the stratification analysis, 72R-carrying genotype conferred a 3.46-fold （95% CI=1.02-11.72） and a 1.05-fold （95% CI=0.36-3.08） increased risk of liver metastases for p53 overexpression-positive and negative colorectal cancers, respectively. Conclusion： These results demonstrate for the first time that the 72R allele of the p53 polymorphism has an increased risk for liver metastases in colorectal cancers positive for p53 overexpression.
基金This work was supported by grants from the National Natural Science Foundation of China[81772974,81972882]Bilateral Inter-Governmental S&T Cooperation Project from Ministry of Science and Technology of China[SQ2018YFE010068]。
文摘Abnormally enhanced de novo lipid biosynthesis has been increasingly realized to play crucial roles in the initiation and progression of varieties of cancers including breast cancer.However,the mechanisms underlying the dysregulation of lipid biosynthesis in breast cancer remain largely unknown.Here,we reported that seryl tRNA synthetase(SerRS),a key enzyme for protein biosynthesis,could translocate into the nucleus in a glucose-dependent manner to suppress key genes involved in the de novo lipid biosynthesis.In normal mammary gland epithelial cells glucose can promote the nuclear translocation of SerRS by increasing the acetylation of SerRS at lysine 323.In SerRS knock-in mice bearing acetylation-defective lysine to arginine mutation.
