摘要
Objective： To evaluate the association between p53 codon 72 polymorphism （R72P） and the risk ofcolorectal liver metastases. Methods： The p53 R72P genotype was identified by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method in 78 consecutive colorectal cancer patients with liver metastases and 214 age- and sex-matched cases with nonmetastatic colorectal cancer. Results： The R allele of the p53 R72P polymorphism was more frequently found in metastatic cases than in nonmetastatic cases （P=0.075）. Carriers of the 72R allele had a 2.25-fold （95% CI （confidence interval）=1.05-4.83） increased risk of liver metastases. On the stratification analysis, 72R-carrying genotype conferred a 3.46-fold （95% CI=1.02-11.72） and a 1.05-fold （95% CI=0.36-3.08） increased risk of liver metastases for p53 overexpression-positive and negative colorectal cancers, respectively. Conclusion： These results demonstrate for the first time that the 72R allele of the p53 polymorphism has an increased risk for liver metastases in colorectal cancers positive for p53 overexpression.
在 72 多型性(R72P ) 和颜色的风险上评估在 p53 鳕鱼之间的协会的目的表面的肝转移。p53 R72P 遗传型被聚合酶链反应限制识别的方法碎裂在 78 连续颜色的长度多型性(PCR-RFLP ) 方法有肝转移和 214 年龄的表面的癌症病人 -- 并且有非变形的颜色的匹配性的盒子表面的癌症。p53 R72P 多型性的 R 等位基因更经常比在非变形的盒子(P=0.075 ) 中在变形情况中被发现的结果。72R 等位基因的搬运人有 2.25 褶层(95% CI (置信区间)= 1.05 ∼4.83 ) 肝转移的增加的风险。在层化分析上， 72R-carrying 遗传型授与 3.46 褶层(95% CI=1.02∼11.72 ) ， 1.05 褶层(95% CI=0.36∼3.08 ) 为 overexpression 积极、否定的 p53 增加了肝转移的风险分别地渲染表面的癌症。这些结果第一次表明的结论 p53 多型性的 72R 等位基因在颜色为肝转移有增加的风险为在表示上的 p53 积极的表面的癌症。
基金
Project supported by the National Natural Science Foundation of China (No. 30470791)
the Medical Science and Technology Research Foundation for the 11th Five-Year Program of People's Liberation Army, Nanjing Branch, China (No. 06MA27)
